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Effect of vascular endothelial growth factor and interleukin-1beta on apoptosis in endometrial cell cultures from patients with endometriosis and controls.
J Reprod Immunol. 2010 Mar; 84(2):193-8.JR

Abstract

The aim of this study was to evaluate the effect of vascular endothelial growth factor (VEGF) and interleukin-1beta (IL-1beta) on apoptosis induced by leuprolide acetate (LA) in endometrial epithelial cell cultures from patients with endometriosis. Primary endometrial epithelial cell cultures were obtained from uterine endometrial biopsies of patients with endometriosis and control women. Endometrial epithelial cells were incubated with LA; a combination of LA and VEGF; a combination of LA and IL-1beta; or in basal conditions. LA was added 3h prior to addition of VEGF and IL-1beta. After stimulation, the percentage of apoptotic cells was evaluated by the acridine orange-ethidium bromide technique and Bax expression was assessed by western blot. Treatment with LA enhanced the percentage of apoptotic cells in endometrial epithelial cells from subjects with endometriosis and control subjects. Addition of either VEGF or IL-1beta after exposure to LA restored the percentage of apoptotic cells to basal levels. Moreover, treatment with LA increased Bax expression in endometrial epithelial cells from patients with endometriosis. This effect was reverted by the addition of either VEGF or IL-1beta. Our results show that VEGF and IL-1beta reduce apoptosis and decrease Bax expression in endometrial epithelial cells from patients with endometriosis. This study suggests that VEGF and IL-1beta may protect endometriotic cells from undergoing apoptosis in addition to exerting their pro-angiogenic role.

Authors+Show Affiliations

Instituto de Biología y Medicina Experimental (IBYME), Vuelta de Obligado 2490, C1428ADN Buenos Aires, Argentina. mabilotas@dna.uba.arNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20137818

Citation

Bilotas, Mariela, et al. "Effect of Vascular Endothelial Growth Factor and Interleukin-1beta On Apoptosis in Endometrial Cell Cultures From Patients With Endometriosis and Controls." Journal of Reproductive Immunology, vol. 84, no. 2, 2010, pp. 193-8.
Bilotas M, Meresman G, Buquet R, et al. Effect of vascular endothelial growth factor and interleukin-1beta on apoptosis in endometrial cell cultures from patients with endometriosis and controls. J Reprod Immunol. 2010;84(2):193-8.
Bilotas, M., Meresman, G., Buquet, R., Sueldo, C., & Barañao, R. I. (2010). Effect of vascular endothelial growth factor and interleukin-1beta on apoptosis in endometrial cell cultures from patients with endometriosis and controls. Journal of Reproductive Immunology, 84(2), 193-8. https://doi.org/10.1016/j.jri.2009.12.002
Bilotas M, et al. Effect of Vascular Endothelial Growth Factor and Interleukin-1beta On Apoptosis in Endometrial Cell Cultures From Patients With Endometriosis and Controls. J Reprod Immunol. 2010;84(2):193-8. PubMed PMID: 20137818.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of vascular endothelial growth factor and interleukin-1beta on apoptosis in endometrial cell cultures from patients with endometriosis and controls. AU - Bilotas,Mariela, AU - Meresman,Gabriela, AU - Buquet,Ricardo, AU - Sueldo,Carlos, AU - Barañao,Rosa Inés, Y1 - 2010/02/04/ PY - 2009/03/17/received PY - 2009/11/18/revised PY - 2009/12/01/accepted PY - 2010/2/9/entrez PY - 2010/2/9/pubmed PY - 2010/9/3/medline SP - 193 EP - 8 JF - Journal of reproductive immunology JO - J Reprod Immunol VL - 84 IS - 2 N2 - The aim of this study was to evaluate the effect of vascular endothelial growth factor (VEGF) and interleukin-1beta (IL-1beta) on apoptosis induced by leuprolide acetate (LA) in endometrial epithelial cell cultures from patients with endometriosis. Primary endometrial epithelial cell cultures were obtained from uterine endometrial biopsies of patients with endometriosis and control women. Endometrial epithelial cells were incubated with LA; a combination of LA and VEGF; a combination of LA and IL-1beta; or in basal conditions. LA was added 3h prior to addition of VEGF and IL-1beta. After stimulation, the percentage of apoptotic cells was evaluated by the acridine orange-ethidium bromide technique and Bax expression was assessed by western blot. Treatment with LA enhanced the percentage of apoptotic cells in endometrial epithelial cells from subjects with endometriosis and control subjects. Addition of either VEGF or IL-1beta after exposure to LA restored the percentage of apoptotic cells to basal levels. Moreover, treatment with LA increased Bax expression in endometrial epithelial cells from patients with endometriosis. This effect was reverted by the addition of either VEGF or IL-1beta. Our results show that VEGF and IL-1beta reduce apoptosis and decrease Bax expression in endometrial epithelial cells from patients with endometriosis. This study suggests that VEGF and IL-1beta may protect endometriotic cells from undergoing apoptosis in addition to exerting their pro-angiogenic role. SN - 1872-7603 UR - https://www.unboundmedicine.com/medline/citation/20137818/Effect_of_vascular_endothelial_growth_factor_and_interleukin_1beta_on_apoptosis_in_endometrial_cell_cultures_from_patients_with_endometriosis_and_controls_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0165-0378(10)00007-0 DB - PRIME DP - Unbound Medicine ER -