Tags

Type your tag names separated by a space and hit enter

Accurate quantitation of glutathione in cell lysates through surface-assisted laser desorption/ionization mass spectrometry using gold nanoparticles.
Nanomedicine. 2010 Aug; 6(4):530-7.N

Abstract

We developed a method for the determination of three aminothiols--cysteine, glutathione (GSH), and homocysteine--using surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS). The analytes were first captured using the unmodified 14-nm gold nanoparticles; N-2-mercaptopropionylglycine-modified gold nanoparticles serving as internal standard were sequentially added, and then the sample was analyzed using SALDI-MS. This approach provided good quantitative linearity of the three analytes (R(2) = approximately 0.99), with good reproducibility (relative standard deviations: <10%), in the analyses of GSH in the lysates of human red blood cells and MCF-7 cancer breast cells in the presence and absence of the anti-inflammatory drug sulfasalazine. The internal-standard SALDI-MS approach provides simplicity, accuracy, and precision to the determination of GSH in cells under drug invasion, to open an avenue for SALDI-MS to be used for the precise quantitative determination of a variety of analytes. From the clinical editor: This paper reports the development of a surface assisted laser desorption/ionization mass spectrometry method to precisely determine aminothiols-cysteine (Cys), glutathione (GSH), and homocysteine (HCys).

Links

Publisher Full Text
Aggregator Full Text

Authors+Show Affiliations

Chiang CK
Department of Chemistry, National Taiwan University, Taipei, Taiwan.
Lin YW
No affiliation info available
Chen WT
No affiliation info available
Chang HT
No affiliation info available

MeSH

ErythrocytesGlutathioneGoldHomocysteineHumansMetal NanoparticlesSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationTumor Cells, Cultured

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20138245

Citation

Chiang, Cheng-Kang, et al. "Accurate Quantitation of Glutathione in Cell Lysates Through Surface-assisted Laser Desorption/ionization Mass Spectrometry Using Gold Nanoparticles." Nanomedicine : Nanotechnology, Biology, and Medicine, vol. 6, no. 4, 2010, pp. 530-7.
Chiang CK, Lin YW, Chen WT, et al. Accurate quantitation of glutathione in cell lysates through surface-assisted laser desorption/ionization mass spectrometry using gold nanoparticles. Nanomedicine. 2010;6(4):530-7.
Chiang, C. K., Lin, Y. W., Chen, W. T., & Chang, H. T. (2010). Accurate quantitation of glutathione in cell lysates through surface-assisted laser desorption/ionization mass spectrometry using gold nanoparticles. Nanomedicine : Nanotechnology, Biology, and Medicine, 6(4), 530-7. https://doi.org/10.1016/j.nano.2010.01.006
Chiang CK, et al. Accurate Quantitation of Glutathione in Cell Lysates Through Surface-assisted Laser Desorption/ionization Mass Spectrometry Using Gold Nanoparticles. Nanomedicine. 2010;6(4):530-7. PubMed PMID: 20138245.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Accurate quantitation of glutathione in cell lysates through surface-assisted laser desorption/ionization mass spectrometry using gold nanoparticles. AU - Chiang,Cheng-Kang, AU - Lin,Yang-Wei, AU - Chen,Wen-Tsen, AU - Chang,Huan-Tsung, Y1 - 2010/02/04/ PY - 2009/10/08/received PY - 2009/12/15/revised PY - 2010/01/19/accepted PY - 2010/2/9/entrez PY - 2010/2/9/pubmed PY - 2011/1/5/medline SP - 530 EP - 7 JF - Nanomedicine : nanotechnology, biology, and medicine JO - Nanomedicine VL - 6 IS - 4 N2 - We developed a method for the determination of three aminothiols--cysteine, glutathione (GSH), and homocysteine--using surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS). The analytes were first captured using the unmodified 14-nm gold nanoparticles; N-2-mercaptopropionylglycine-modified gold nanoparticles serving as internal standard were sequentially added, and then the sample was analyzed using SALDI-MS. This approach provided good quantitative linearity of the three analytes (R(2) = approximately 0.99), with good reproducibility (relative standard deviations: <10%), in the analyses of GSH in the lysates of human red blood cells and MCF-7 cancer breast cells in the presence and absence of the anti-inflammatory drug sulfasalazine. The internal-standard SALDI-MS approach provides simplicity, accuracy, and precision to the determination of GSH in cells under drug invasion, to open an avenue for SALDI-MS to be used for the precise quantitative determination of a variety of analytes. From the clinical editor: This paper reports the development of a surface assisted laser desorption/ionization mass spectrometry method to precisely determine aminothiols-cysteine (Cys), glutathione (GSH), and homocysteine (HCys). SN - 1549-9642 UR - https://www.unboundmedicine.com/medline/citation/20138245/Accurate_quantitation_of_glutathione_in_cell_lysates_through_surface_assisted_laser_desorption/ionization_mass_spectrometry_using_gold_nanoparticles_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1549-9634(10)00013-4 DB - PRIME DP - Unbound Medicine ER -