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Ammodytoxin content of Vipera ammodytes ammodytes venom as a prognostic factor for venom immunogenicity.
Comp Biochem Physiol C Toxicol Pharmacol. 2010 May; 151(4):455-60.CB

Abstract

Venoms are complex mixtures of proteins, peptides and other compounds whose biochemical and biological variability has been clearly demonstrated. These molecules have been used as antigens for immunization of anti-venom-producing animals (horses or sheep). Ammodytoxins (Atx) are potently neurotoxic compounds, and the most toxic compounds isolated so far from the Vipera ammodytes ammodytes (Vaa) venom. Recently we have shown that the level of antibodies specific to Vaa venom's most toxic component, ammodytoxin A (AtxA), (anti-AtxA IgG) in Vaa venom immunized rabbit sera highly correlated to the venom toxicity-neutralization potential of these sera. Here we investigated whether Atx content of Vaa venom could influence the outcome of immunization procedure. The novel ELISA was developed for precise determination of Atx content and Atx was quantified in venom samples used for immunization of rabbits. We clearly showed that animals immunized with the venom containing lower amount of Atx produced sera with significantly lower venom toxicity-neutralizing power and, vice versa, animals immunized with venoms containing higher amount of Atx produced sera with higher venom toxicity-neutralizing ability. Thus, the content of Atx in Vaa venom is a relevant parameter of its suitability in the production of highly protective Vaa anti-venom.

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Authors+Show Affiliations

Halassy B
Research and Development Department, Institute of Immunology, Inc., Rockefellerova 10, HR-10 000 Zagreb, Croatia. bhalassy@imz.hr
Habjanec L
No affiliation info available
Balija ML
No affiliation info available
Kurtović T
No affiliation info available
Brgles M
No affiliation info available
Krizaj I
No affiliation info available

MeSH

AnimalsAntiveninsChromatography, High Pressure LiquidEnzyme-Linked Immunosorbent AssayFemaleHemorrhageImmunologic FactorsLethal Dose 50MicePhospholipasesRabbitsRatsRats, Inbred LewViper VenomsViperidae

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20139032

Citation

Halassy, Beata, et al. "Ammodytoxin Content of Vipera Ammodytes Ammodytes Venom as a Prognostic Factor for Venom Immunogenicity." Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP, vol. 151, no. 4, 2010, pp. 455-60.
Halassy B, Habjanec L, Balija ML, et al. Ammodytoxin content of Vipera ammodytes ammodytes venom as a prognostic factor for venom immunogenicity. Comp Biochem Physiol C Toxicol Pharmacol. 2010;151(4):455-60.
Halassy, B., Habjanec, L., Balija, M. L., Kurtović, T., Brgles, M., & Krizaj, I. (2010). Ammodytoxin content of Vipera ammodytes ammodytes venom as a prognostic factor for venom immunogenicity. Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP, 151(4), 455-60. https://doi.org/10.1016/j.cbpc.2010.02.001
Halassy B, et al. Ammodytoxin Content of Vipera Ammodytes Ammodytes Venom as a Prognostic Factor for Venom Immunogenicity. Comp Biochem Physiol C Toxicol Pharmacol. 2010;151(4):455-60. PubMed PMID: 20139032.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ammodytoxin content of Vipera ammodytes ammodytes venom as a prognostic factor for venom immunogenicity. AU - Halassy,Beata, AU - Habjanec,Lidija, AU - Balija,Maja Lang, AU - Kurtović,Tihana, AU - Brgles,Marija, AU - Krizaj,Igor, Y1 - 2010/02/06/ PY - 2009/12/22/received PY - 2010/02/01/revised PY - 2010/02/01/accepted PY - 2010/2/9/entrez PY - 2010/2/9/pubmed PY - 2010/6/9/medline SP - 455 EP - 60 JF - Comparative biochemistry and physiology. Toxicology & pharmacology : CBP JO - Comp Biochem Physiol C Toxicol Pharmacol VL - 151 IS - 4 N2 - Venoms are complex mixtures of proteins, peptides and other compounds whose biochemical and biological variability has been clearly demonstrated. These molecules have been used as antigens for immunization of anti-venom-producing animals (horses or sheep). Ammodytoxins (Atx) are potently neurotoxic compounds, and the most toxic compounds isolated so far from the Vipera ammodytes ammodytes (Vaa) venom. Recently we have shown that the level of antibodies specific to Vaa venom's most toxic component, ammodytoxin A (AtxA), (anti-AtxA IgG) in Vaa venom immunized rabbit sera highly correlated to the venom toxicity-neutralization potential of these sera. Here we investigated whether Atx content of Vaa venom could influence the outcome of immunization procedure. The novel ELISA was developed for precise determination of Atx content and Atx was quantified in venom samples used for immunization of rabbits. We clearly showed that animals immunized with the venom containing lower amount of Atx produced sera with significantly lower venom toxicity-neutralizing power and, vice versa, animals immunized with venoms containing higher amount of Atx produced sera with higher venom toxicity-neutralizing ability. Thus, the content of Atx in Vaa venom is a relevant parameter of its suitability in the production of highly protective Vaa anti-venom. SN - 1532-0456 UR - https://www.unboundmedicine.com/medline/citation/20139032/Ammodytoxin_content_of_Vipera_ammodytes_ammodytes_venom_as_a_prognostic_factor_for_venom_immunogenicity_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1532-0456(10)00022-0 DB - PRIME DP - Unbound Medicine ER -