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Molecular characterization of novel low-molecular-weight glutenin genes in Aegilops longissima.
J Appl Genet. 2010; 51(1):9-18.JA

Abstract

Extensive genetic variations of low-molecular-weight glutenin subunits (LMW-GS) and their coding genes were found in the wild diploid A- and D-genome donors of common wheat. In this study, we reported the isolation and characterization of 8 novel LMW-GS genes from Ae.longissima Schweinf. & Muschl., a species of the section Sitopsis of the genus Aegilops, which is closely related to the B genome of common wheat. Based on the N-terminal domain sequences, the 8 genes were divided into 3 groups. A consensus alignment of the extremely conserved domains with known gene groups and the subsequent cluster analysis showed that 2 out of the 3 groups of LMW-GS genes were closely related to those from the B genome, and the remaining was related to those from A and D genomes of wheat and Ae. tauschii. Using 3 sets of gene-group-specific primers, PCRs in diploid, tetraploid and hexaploid wheats and Ae. tauschii failed to obtain the expected products, indicating that the 3 groups of LMW-GS genes obtained in this study were new members of LMW-GS multi-gene families. These results suggested that the Sitopsis species of the genus Aegilops with novel gene variations could be used as valuable gene resources of LMW-GS. The 3 sets of group-specific primers could be utilized as molecular markers to investigate the introgression of novel alien LMW-GS genes from Ae. longissima into wheat.

Authors+Show Affiliations

Dujiangyan Campus, Sichuan Agricultural University, Dujiangyan, Sichuan, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20145295

Citation

Huang, Z, et al. "Molecular Characterization of Novel Low-molecular-weight Glutenin Genes in Aegilops Longissima." Journal of Applied Genetics, vol. 51, no. 1, 2010, pp. 9-18.
Huang Z, Long H, Jiang QT, et al. Molecular characterization of novel low-molecular-weight glutenin genes in Aegilops longissima. J Appl Genet. 2010;51(1):9-18.
Huang, Z., Long, H., Jiang, Q. T., Wei, Y. M., Yan, Z. H., & Zheng, Y. L. (2010). Molecular characterization of novel low-molecular-weight glutenin genes in Aegilops longissima. Journal of Applied Genetics, 51(1), 9-18. https://doi.org/10.1007/BF03195705
Huang Z, et al. Molecular Characterization of Novel Low-molecular-weight Glutenin Genes in Aegilops Longissima. J Appl Genet. 2010;51(1):9-18. PubMed PMID: 20145295.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular characterization of novel low-molecular-weight glutenin genes in Aegilops longissima. AU - Huang,Z, AU - Long,H, AU - Jiang,Q-T, AU - Wei,Y-M, AU - Yan,Z-H, AU - Zheng,Y-L, PY - 2010/2/11/entrez PY - 2010/2/11/pubmed PY - 2010/5/19/medline SP - 9 EP - 18 JF - Journal of applied genetics JO - J Appl Genet VL - 51 IS - 1 N2 - Extensive genetic variations of low-molecular-weight glutenin subunits (LMW-GS) and their coding genes were found in the wild diploid A- and D-genome donors of common wheat. In this study, we reported the isolation and characterization of 8 novel LMW-GS genes from Ae.longissima Schweinf. & Muschl., a species of the section Sitopsis of the genus Aegilops, which is closely related to the B genome of common wheat. Based on the N-terminal domain sequences, the 8 genes were divided into 3 groups. A consensus alignment of the extremely conserved domains with known gene groups and the subsequent cluster analysis showed that 2 out of the 3 groups of LMW-GS genes were closely related to those from the B genome, and the remaining was related to those from A and D genomes of wheat and Ae. tauschii. Using 3 sets of gene-group-specific primers, PCRs in diploid, tetraploid and hexaploid wheats and Ae. tauschii failed to obtain the expected products, indicating that the 3 groups of LMW-GS genes obtained in this study were new members of LMW-GS multi-gene families. These results suggested that the Sitopsis species of the genus Aegilops with novel gene variations could be used as valuable gene resources of LMW-GS. The 3 sets of group-specific primers could be utilized as molecular markers to investigate the introgression of novel alien LMW-GS genes from Ae. longissima into wheat. SN - 1234-1983 UR - https://www.unboundmedicine.com/medline/citation/20145295/Molecular_characterization_of_novel_low_molecular_weight_glutenin_genes_in_Aegilops_longissima_ L2 - https://dx.doi.org/10.1007/BF03195705 DB - PRIME DP - Unbound Medicine ER -