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Hydroxytyrosol protects against oxidative damage by simultaneous activation of mitochondrial biogenesis and phase II detoxifying enzyme systems in retinal pigment epithelial cells.
J Nutr Biochem. 2010 Nov; 21(11):1089-98.JN

Abstract

Studies in this laboratory have previously shown that hydroxytyrosol, the major antioxidant polyphenol in olives, protects ARPE-19 human retinal pigment epithelial cells from oxidative damage induced by acrolein, an environmental toxin and endogenous end product of lipid oxidation, that occurs at increased levels in age-related macular degeneration lesions. A proposed mechanism for this is that protection by hydroxytyrosol against oxidative stress is conferred by the simultaneous activation of two critically important pathways, viz., induction of phase II detoxifying enzymes and stimulation of mitochondrial biogenesis. Cultured ARPE-19 cells were pretreated with hydroxytyrosol and challenged with acrolein. The protective effects of hydroxytyrosol on key factors of mitochondrial biogenesis and phase II detoxifying enzyme systems were examined. Hydroxytyrosol treatment simultaneously protected against acrolein-induced inhibition of nuclear factor-E2-related factor 2 (Nrf2) and peroxisome proliferator-activated receptor coactivator 1 alpha (PPARGC1α) in ARPE-19 cells. The activation of Nrf2 led to activation of phase II detoxifying enzymes, including γ-glutamyl-cysteinyl-ligase, NADPH (nicotinamide adenine dinucleotide phosphate)-quinone-oxidoreductase 1, heme-oxygenase-1, superoxide dismutase, peroxiredoxin and thioredoxin as well as other antioxidant enzymes, while the activation of PPARGC1α led to increased protein expression of mitochondrial transcription factor A, uncoupling protein 2 and mitochondrial complexes. These results suggest that hydroxytyrosol is a potent inducer of phase II detoxifying enzymes and an enhancer of mitochondrial biogenesis. Dietary supplementation of hydroxytyrosol may contribute to eye health by preventing the degeneration of retinal pigment epithelial cells induced by oxidative stress.

Authors+Show Affiliations

Institute for Nutritional Science, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20149621

Citation

Zhu, Lu, et al. "Hydroxytyrosol Protects Against Oxidative Damage By Simultaneous Activation of Mitochondrial Biogenesis and Phase II Detoxifying Enzyme Systems in Retinal Pigment Epithelial Cells." The Journal of Nutritional Biochemistry, vol. 21, no. 11, 2010, pp. 1089-98.
Zhu L, Liu Z, Feng Z, et al. Hydroxytyrosol protects against oxidative damage by simultaneous activation of mitochondrial biogenesis and phase II detoxifying enzyme systems in retinal pigment epithelial cells. J Nutr Biochem. 2010;21(11):1089-98.
Zhu, L., Liu, Z., Feng, Z., Hao, J., Shen, W., Li, X., Sun, L., Sharman, E., Wang, Y., Wertz, K., Weber, P., Shi, X., & Liu, J. (2010). Hydroxytyrosol protects against oxidative damage by simultaneous activation of mitochondrial biogenesis and phase II detoxifying enzyme systems in retinal pigment epithelial cells. The Journal of Nutritional Biochemistry, 21(11), 1089-98. https://doi.org/10.1016/j.jnutbio.2009.09.006
Zhu L, et al. Hydroxytyrosol Protects Against Oxidative Damage By Simultaneous Activation of Mitochondrial Biogenesis and Phase II Detoxifying Enzyme Systems in Retinal Pigment Epithelial Cells. J Nutr Biochem. 2010;21(11):1089-98. PubMed PMID: 20149621.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hydroxytyrosol protects against oxidative damage by simultaneous activation of mitochondrial biogenesis and phase II detoxifying enzyme systems in retinal pigment epithelial cells. AU - Zhu,Lu, AU - Liu,Zhongbo, AU - Feng,Zhihui, AU - Hao,Jiejie, AU - Shen,Weili, AU - Li,Xuesen, AU - Sun,Lijuan, AU - Sharman,Edward, AU - Wang,Ying, AU - Wertz,Karin, AU - Weber,Peter, AU - Shi,Xianglin, AU - Liu,Jiankang, Y1 - 2010/02/09/ PY - 2009/03/18/received PY - 2009/09/04/revised PY - 2009/09/14/accepted PY - 2010/2/13/entrez PY - 2010/2/13/pubmed PY - 2011/1/29/medline SP - 1089 EP - 98 JF - The Journal of nutritional biochemistry JO - J. Nutr. Biochem. VL - 21 IS - 11 N2 - Studies in this laboratory have previously shown that hydroxytyrosol, the major antioxidant polyphenol in olives, protects ARPE-19 human retinal pigment epithelial cells from oxidative damage induced by acrolein, an environmental toxin and endogenous end product of lipid oxidation, that occurs at increased levels in age-related macular degeneration lesions. A proposed mechanism for this is that protection by hydroxytyrosol against oxidative stress is conferred by the simultaneous activation of two critically important pathways, viz., induction of phase II detoxifying enzymes and stimulation of mitochondrial biogenesis. Cultured ARPE-19 cells were pretreated with hydroxytyrosol and challenged with acrolein. The protective effects of hydroxytyrosol on key factors of mitochondrial biogenesis and phase II detoxifying enzyme systems were examined. Hydroxytyrosol treatment simultaneously protected against acrolein-induced inhibition of nuclear factor-E2-related factor 2 (Nrf2) and peroxisome proliferator-activated receptor coactivator 1 alpha (PPARGC1α) in ARPE-19 cells. The activation of Nrf2 led to activation of phase II detoxifying enzymes, including γ-glutamyl-cysteinyl-ligase, NADPH (nicotinamide adenine dinucleotide phosphate)-quinone-oxidoreductase 1, heme-oxygenase-1, superoxide dismutase, peroxiredoxin and thioredoxin as well as other antioxidant enzymes, while the activation of PPARGC1α led to increased protein expression of mitochondrial transcription factor A, uncoupling protein 2 and mitochondrial complexes. These results suggest that hydroxytyrosol is a potent inducer of phase II detoxifying enzymes and an enhancer of mitochondrial biogenesis. Dietary supplementation of hydroxytyrosol may contribute to eye health by preventing the degeneration of retinal pigment epithelial cells induced by oxidative stress. SN - 1873-4847 UR - https://www.unboundmedicine.com/medline/citation/20149621/Hydroxytyrosol_protects_against_oxidative_damage_by_simultaneous_activation_of_mitochondrial_biogenesis_and_phase_II_detoxifying_enzyme_systems_in_retinal_pigment_epithelial_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0955-2863(09)00206-X DB - PRIME DP - Unbound Medicine ER -