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The cannabinoid receptor agonist WIN 55,212-2 inhibits antigen-induced plasma extravasation in guinea pig airways.
Int Arch Allergy Immunol. 2010; 152(3):295-300.IA

Abstract

BACKGROUND

Although neurogenic inflammation of the airways via activation of C-fibers is thought to be important in the pathogenesis of asthma, the mechanisms regulating C-fiber activity remain uncertain.

OBJECTIVE

The influence of a cannabinoid receptor agonist, WIN 55,212-2, on C-fiber activation in guinea pig airways was investigated, as was the mechanism by which cannabinoids regulate antigen-induced airway inflammation.

METHODS

The inhibitory effect of WIN 55,212-2 on antigen-induced plasma extravasation was assessed in guinea pig tracheal tissues by photometric measurement of extravasated Evans blue dye after extraction with formamide.

RESULTS

Pretreatment with WIN 55,212-2 (0.001, 0.01 or 0.1 mg/kg) significantly and dose-dependently reduced tracheal plasma extravasation induced by inhaling a 5% ovalbumin solution for 2 min after pretreatment with a neutral endopeptidedase inhibitor (phosphoramidon at 2.5 mg/kg i.v.). A cannabinoid CB2 receptor antagonist (SR144528) blunted the inhibitory effect of WIN 55,212-2, while a cannabinoid CB1 antagonist (SR141716A) did not. Pretreatment with a neurokinin-1 receptor antagonist (FK888) significantly reduced ovalbumin-induced extravasation of Evans blue dye. Pretreatment with the combination of WIN 55,212-2 and FK888 reduced antigen-induced plasma extravasation more markedly than FK888 alone.

CONCLUSIONS

These findings suggest that WIN 55,212-2 inhibits C-fiber activation via the cannabinoid CB2 receptor and thus suppresses antigen-induced inflammation in guinea pig airways.

Authors+Show Affiliations

Department of Pediatrics, Dokkyo Medical University, Tochigi, Japan.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20150748

Citation

Fukuda, Hironobu, et al. "The Cannabinoid Receptor Agonist WIN 55,212-2 Inhibits Antigen-induced Plasma Extravasation in Guinea Pig Airways." International Archives of Allergy and Immunology, vol. 152, no. 3, 2010, pp. 295-300.
Fukuda H, Abe T, Yoshihara S. The cannabinoid receptor agonist WIN 55,212-2 inhibits antigen-induced plasma extravasation in guinea pig airways. Int Arch Allergy Immunol. 2010;152(3):295-300.
Fukuda, H., Abe, T., & Yoshihara, S. (2010). The cannabinoid receptor agonist WIN 55,212-2 inhibits antigen-induced plasma extravasation in guinea pig airways. International Archives of Allergy and Immunology, 152(3), 295-300. https://doi.org/10.1159/000283042
Fukuda H, Abe T, Yoshihara S. The Cannabinoid Receptor Agonist WIN 55,212-2 Inhibits Antigen-induced Plasma Extravasation in Guinea Pig Airways. Int Arch Allergy Immunol. 2010;152(3):295-300. PubMed PMID: 20150748.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The cannabinoid receptor agonist WIN 55,212-2 inhibits antigen-induced plasma extravasation in guinea pig airways. AU - Fukuda,Hironobu, AU - Abe,Toshio, AU - Yoshihara,Shigemi, Y1 - 2010/02/12/ PY - 2009/05/05/received PY - 2009/09/11/accepted PY - 2010/2/13/entrez PY - 2010/2/13/pubmed PY - 2010/8/10/medline SP - 295 EP - 300 JF - International archives of allergy and immunology JO - Int. Arch. Allergy Immunol. VL - 152 IS - 3 N2 - BACKGROUND: Although neurogenic inflammation of the airways via activation of C-fibers is thought to be important in the pathogenesis of asthma, the mechanisms regulating C-fiber activity remain uncertain. OBJECTIVE: The influence of a cannabinoid receptor agonist, WIN 55,212-2, on C-fiber activation in guinea pig airways was investigated, as was the mechanism by which cannabinoids regulate antigen-induced airway inflammation. METHODS: The inhibitory effect of WIN 55,212-2 on antigen-induced plasma extravasation was assessed in guinea pig tracheal tissues by photometric measurement of extravasated Evans blue dye after extraction with formamide. RESULTS: Pretreatment with WIN 55,212-2 (0.001, 0.01 or 0.1 mg/kg) significantly and dose-dependently reduced tracheal plasma extravasation induced by inhaling a 5% ovalbumin solution for 2 min after pretreatment with a neutral endopeptidedase inhibitor (phosphoramidon at 2.5 mg/kg i.v.). A cannabinoid CB2 receptor antagonist (SR144528) blunted the inhibitory effect of WIN 55,212-2, while a cannabinoid CB1 antagonist (SR141716A) did not. Pretreatment with a neurokinin-1 receptor antagonist (FK888) significantly reduced ovalbumin-induced extravasation of Evans blue dye. Pretreatment with the combination of WIN 55,212-2 and FK888 reduced antigen-induced plasma extravasation more markedly than FK888 alone. CONCLUSIONS: These findings suggest that WIN 55,212-2 inhibits C-fiber activation via the cannabinoid CB2 receptor and thus suppresses antigen-induced inflammation in guinea pig airways. SN - 1423-0097 UR - https://www.unboundmedicine.com/medline/citation/20150748/The_cannabinoid_receptor_agonist_WIN_55212_2_inhibits_antigen_induced_plasma_extravasation_in_guinea_pig_airways_ L2 - https://www.karger.com?DOI=10.1159/000283042 DB - PRIME DP - Unbound Medicine ER -