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Quantitative determination of oxybutynin hydrochloride by spectrophotometry, chemometry and HPTLC in presence of its degradation product and additives in different pharmaceutical dosage forms.
Talanta 2010; 80(5):2007-15T

Abstract

Simple, accurate, sensitive and validated UV spectrophotometric, chemometric and HPTLC-densitometric methods were developed for determination of oxybutynin hydrochloride (OX) in presence of its degradation product and additives in its pharmaceutical formulations. Method A is the first derivative of ratio spectra (DD(1)) which allows the determination of OX in presence of its degradate in pure form and tablets by measuring the peaks amplitude at 216 nm. Method B and C are principal component regression (PCR) and partial least-squares (PLS) for determination of OX in presence of its degradate in pure form, tablets and syrup. While, the developed high performance thin layer chromatography HPTLC-densitometric method was based on the separation of OX from its degradation product, methylparaben and propylparaben followed by densitometric measurement at 220 nm which allows the determination of OX in pure form, tablets and syrup. The separation was achieved using HPTLC silica gel F(254) plates and chloroform:methanol:ammonia solution:triethylamine (100:3:0.5:0.2, v/v/v/v) as the developing system. The accuracy, precision and linearity ranges of the developed methods were determined. The results obtained were statistically compared with each other and to that of a reported HPLC method, and there was no significant difference between the proposed methods and the reported method regarding both accuracy and precision.

Authors+Show Affiliations

Analytical Chemistry Department, Faculty of Pharmacy, Beni-Suef University, Egypt.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Validation Studies

Language

eng

PubMed ID

20152446

Citation

Wagieh, Nour E., et al. "Quantitative Determination of Oxybutynin Hydrochloride By Spectrophotometry, Chemometry and HPTLC in Presence of Its Degradation Product and Additives in Different Pharmaceutical Dosage Forms." Talanta, vol. 80, no. 5, 2010, pp. 2007-15.
Wagieh NE, Hegazy MA, Abdelkawy M, et al. Quantitative determination of oxybutynin hydrochloride by spectrophotometry, chemometry and HPTLC in presence of its degradation product and additives in different pharmaceutical dosage forms. Talanta. 2010;80(5):2007-15.
Wagieh, N. E., Hegazy, M. A., Abdelkawy, M., & Abdelaleem, E. A. (2010). Quantitative determination of oxybutynin hydrochloride by spectrophotometry, chemometry and HPTLC in presence of its degradation product and additives in different pharmaceutical dosage forms. Talanta, 80(5), pp. 2007-15. doi:10.1016/j.talanta.2009.11.002.
Wagieh NE, et al. Quantitative Determination of Oxybutynin Hydrochloride By Spectrophotometry, Chemometry and HPTLC in Presence of Its Degradation Product and Additives in Different Pharmaceutical Dosage Forms. Talanta. 2010 Mar 15;80(5):2007-15. PubMed PMID: 20152446.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantitative determination of oxybutynin hydrochloride by spectrophotometry, chemometry and HPTLC in presence of its degradation product and additives in different pharmaceutical dosage forms. AU - Wagieh,Nour E, AU - Hegazy,Maha A, AU - Abdelkawy,M, AU - Abdelaleem,Eglal A, Y1 - 2009/11/10/ PY - 2009/08/04/received PY - 2009/10/15/revised PY - 2009/11/02/accepted PY - 2010/2/16/entrez PY - 2010/2/16/pubmed PY - 2010/5/19/medline SP - 2007 EP - 15 JF - Talanta JO - Talanta VL - 80 IS - 5 N2 - Simple, accurate, sensitive and validated UV spectrophotometric, chemometric and HPTLC-densitometric methods were developed for determination of oxybutynin hydrochloride (OX) in presence of its degradation product and additives in its pharmaceutical formulations. Method A is the first derivative of ratio spectra (DD(1)) which allows the determination of OX in presence of its degradate in pure form and tablets by measuring the peaks amplitude at 216 nm. Method B and C are principal component regression (PCR) and partial least-squares (PLS) for determination of OX in presence of its degradate in pure form, tablets and syrup. While, the developed high performance thin layer chromatography HPTLC-densitometric method was based on the separation of OX from its degradation product, methylparaben and propylparaben followed by densitometric measurement at 220 nm which allows the determination of OX in pure form, tablets and syrup. The separation was achieved using HPTLC silica gel F(254) plates and chloroform:methanol:ammonia solution:triethylamine (100:3:0.5:0.2, v/v/v/v) as the developing system. The accuracy, precision and linearity ranges of the developed methods were determined. The results obtained were statistically compared with each other and to that of a reported HPLC method, and there was no significant difference between the proposed methods and the reported method regarding both accuracy and precision. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/20152446/Quantitative_determination_of_oxybutynin_hydrochloride_by_spectrophotometry_chemometry_and_HPTLC_in_presence_of_its_degradation_product_and_additives_in_different_pharmaceutical_dosage_forms_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-9140(09)00851-0 DB - PRIME DP - Unbound Medicine ER -