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A novel low-cost and easy to develop functionalization platform. Case study: aptamer-based detection of thrombin by surface plasmon resonance.
Talanta. 2010 Mar 15; 80(5):2157-64.T

Abstract

A novel low-cost platform to assess biomolecular interactions was investigated using surface plasmon resonance and an aptamer-based assay for thrombin detection. Gold SPR surface functionalized with a carboxylated cross-linked BSA film (cBSA) and commercially available carboxymethylated dextran chip (CM5) were used as immobilization platforms for the thrombin binding aptamer. The high end commercial instrument Biacore 3000 and a custom made FIA set-up involving TI Spreeta sensor (TSPR2K23) were used to assess different concentrations of thrombin within the range 0.1-150 nM both in buffer and in a complex matrix (plasma) using the obtained aptasensors. Based on data derived from both CM5 and cBSA platforms, the cBSA aptasensor exhibited good selectivity, stability and regeneration ability, both in buffer and in complex matrices (plasma), comparable with CM5.

Authors+Show Affiliations

International Centre of Biodynamics, Intrarea Portocalelor 1B, 060101 Bucharest, Romania.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20152466

Citation

Polonschii, Cristina, et al. "A Novel Low-cost and Easy to Develop Functionalization Platform. Case Study: Aptamer-based Detection of Thrombin By Surface Plasmon Resonance." Talanta, vol. 80, no. 5, 2010, pp. 2157-64.
Polonschii C, David S, Tombelli S, et al. A novel low-cost and easy to develop functionalization platform. Case study: aptamer-based detection of thrombin by surface plasmon resonance. Talanta. 2010;80(5):2157-64.
Polonschii, C., David, S., Tombelli, S., Mascini, M., & Gheorghiu, M. (2010). A novel low-cost and easy to develop functionalization platform. Case study: aptamer-based detection of thrombin by surface plasmon resonance. Talanta, 80(5), 2157-64. https://doi.org/10.1016/j.talanta.2009.11.023
Polonschii C, et al. A Novel Low-cost and Easy to Develop Functionalization Platform. Case Study: Aptamer-based Detection of Thrombin By Surface Plasmon Resonance. Talanta. 2010 Mar 15;80(5):2157-64. PubMed PMID: 20152466.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A novel low-cost and easy to develop functionalization platform. Case study: aptamer-based detection of thrombin by surface plasmon resonance. AU - Polonschii,Cristina, AU - David,Sorin, AU - Tombelli,Sara, AU - Mascini,Marco, AU - Gheorghiu,Mihaela, Y1 - 2009/11/12/ PY - 2009/08/04/received PY - 2009/10/27/revised PY - 2009/11/05/accepted PY - 2010/2/16/entrez PY - 2010/2/16/pubmed PY - 2010/5/19/medline SP - 2157 EP - 64 JF - Talanta JO - Talanta VL - 80 IS - 5 N2 - A novel low-cost platform to assess biomolecular interactions was investigated using surface plasmon resonance and an aptamer-based assay for thrombin detection. Gold SPR surface functionalized with a carboxylated cross-linked BSA film (cBSA) and commercially available carboxymethylated dextran chip (CM5) were used as immobilization platforms for the thrombin binding aptamer. The high end commercial instrument Biacore 3000 and a custom made FIA set-up involving TI Spreeta sensor (TSPR2K23) were used to assess different concentrations of thrombin within the range 0.1-150 nM both in buffer and in a complex matrix (plasma) using the obtained aptasensors. Based on data derived from both CM5 and cBSA platforms, the cBSA aptasensor exhibited good selectivity, stability and regeneration ability, both in buffer and in complex matrices (plasma), comparable with CM5. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/20152466/A_novel_low_cost_and_easy_to_develop_functionalization_platform__Case_study:_aptamer_based_detection_of_thrombin_by_surface_plasmon_resonance_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-9140(09)00872-8 DB - PRIME DP - Unbound Medicine ER -