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Identification and characterization of the major allergen of green bean (Phaseolus vulgaris) as a non-specific lipid transfer protein (Pha v 3).
Mol Immunol. 2010 Apr; 47(7-8):1561-8.MI

Abstract

BACKGROUND

Green bean (GB) has been reported to cause allergic reactions after ingestion, contact or inhalation of particles deriving from processing or cooking. Up-to-date no food allergens have been fully characterized in GB.

OBJECTIVE

To characterize the GB major allergen(s) on a molecular level and to verify the involvement of non-specific lipid transfer proteins (nsLTPs) in GB allergy.

METHODS

We recruited 10 Spanish patients reporting adverse reactions to GB. Skin prick tests, specific IgE detection and oral provocation were performed. Two nsLTP cDNAs were cloned from GB and over-expressed in Pichia pastoris. The recombinant LTPs (rLTPs) were characterized by circular dichroism spectroscopy and IgE-binding assays (immunoblotting and ELISA) with the patients' sera. Three natural LTPs (nLTPs) were further purified from GB fruit by chromatography. In vitro histamine release test was applied to compare the allergenic potency of rLTPs and nLTPs.

RESULTS

Oral provocation test confirmed GB allergy. A 10kDa protein in GB extract was recognized by 80% of the sera and identified as nsLTP. The two rLTPs (named LTP1a and LTP1b), share 61.3% aa identity and present the typical nsLTP-like secondary structure. The IgE-binding and histamine release assays provided evidence that rLTPs and nLTPs possess different allergenic potency.

CONCLUSIONS

nsLTP (Pha v 3) is the major allergen in GB and constitute a potential risk for patients affected by LTP-syndrome. GB encodes for several LTPs with different immune reactivity.

Authors+Show Affiliations

University of Verona, Department of Biotechnology, Strada Le Grazie, 15-CV1, I-37134 Verona, Italy. gianni.zoccatelli@univr.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20153058

Citation

Zoccatelli, Gianni, et al. "Identification and Characterization of the Major Allergen of Green Bean (Phaseolus Vulgaris) as a Non-specific Lipid Transfer Protein (Pha V 3)." Molecular Immunology, vol. 47, no. 7-8, 2010, pp. 1561-8.
Zoccatelli G, Pokoj S, Foetisch K, et al. Identification and characterization of the major allergen of green bean (Phaseolus vulgaris) as a non-specific lipid transfer protein (Pha v 3). Mol Immunol. 2010;47(7-8):1561-8.
Zoccatelli, G., Pokoj, S., Foetisch, K., Bartra, J., Valero, A., Del Mar San Miguel-Moncin, M., Vieths, S., & Scheurer, S. (2010). Identification and characterization of the major allergen of green bean (Phaseolus vulgaris) as a non-specific lipid transfer protein (Pha v 3). Molecular Immunology, 47(7-8), 1561-8. https://doi.org/10.1016/j.molimm.2010.01.009
Zoccatelli G, et al. Identification and Characterization of the Major Allergen of Green Bean (Phaseolus Vulgaris) as a Non-specific Lipid Transfer Protein (Pha V 3). Mol Immunol. 2010;47(7-8):1561-8. PubMed PMID: 20153058.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification and characterization of the major allergen of green bean (Phaseolus vulgaris) as a non-specific lipid transfer protein (Pha v 3). AU - Zoccatelli,Gianni, AU - Pokoj,Sven, AU - Foetisch,Kay, AU - Bartra,Joan, AU - Valero,Antonio, AU - Del Mar San Miguel-Moncin,Maria, AU - Vieths,Stefan, AU - Scheurer,Stephan, Y1 - 2010/02/11/ PY - 2009/12/21/received PY - 2010/01/17/accepted PY - 2010/2/16/entrez PY - 2010/2/16/pubmed PY - 2010/4/23/medline SP - 1561 EP - 8 JF - Molecular immunology JO - Mol Immunol VL - 47 IS - 7-8 N2 - BACKGROUND: Green bean (GB) has been reported to cause allergic reactions after ingestion, contact or inhalation of particles deriving from processing or cooking. Up-to-date no food allergens have been fully characterized in GB. OBJECTIVE: To characterize the GB major allergen(s) on a molecular level and to verify the involvement of non-specific lipid transfer proteins (nsLTPs) in GB allergy. METHODS: We recruited 10 Spanish patients reporting adverse reactions to GB. Skin prick tests, specific IgE detection and oral provocation were performed. Two nsLTP cDNAs were cloned from GB and over-expressed in Pichia pastoris. The recombinant LTPs (rLTPs) were characterized by circular dichroism spectroscopy and IgE-binding assays (immunoblotting and ELISA) with the patients' sera. Three natural LTPs (nLTPs) were further purified from GB fruit by chromatography. In vitro histamine release test was applied to compare the allergenic potency of rLTPs and nLTPs. RESULTS: Oral provocation test confirmed GB allergy. A 10kDa protein in GB extract was recognized by 80% of the sera and identified as nsLTP. The two rLTPs (named LTP1a and LTP1b), share 61.3% aa identity and present the typical nsLTP-like secondary structure. The IgE-binding and histamine release assays provided evidence that rLTPs and nLTPs possess different allergenic potency. CONCLUSIONS: nsLTP (Pha v 3) is the major allergen in GB and constitute a potential risk for patients affected by LTP-syndrome. GB encodes for several LTPs with different immune reactivity. SN - 1872-9142 UR - https://www.unboundmedicine.com/medline/citation/20153058/Identification_and_characterization_of_the_major_allergen_of_green_bean__Phaseolus_vulgaris__as_a_non_specific_lipid_transfer_protein__Pha_v_3__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0161-5890(10)00021-0 DB - PRIME DP - Unbound Medicine ER -