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Simultaneous quantitative determination of olmesartan and hydrochlorothiazide in human plasma and urine by liquid chromatography coupled to tandem mass spectrometry.
J Chromatogr B Analyt Technol Biomed Life Sci. 2010 Mar 15; 878(9-10):743-8.JC

Abstract

A specific, sensitive and rapid method based on high performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS) was developed for the simultaneous determination of olmesartan (OLM) and hydrochlorothiazide (HCTZ) in human plasma and urine. Solid-phase extraction (SPE) was used to isolate the analytes from biological matrices followed by injection of the extracts onto a C(18) column with isocratic elution. Detection was carried out on a triple quadrupole tandem mass spectrometer in multiple reaction monitoring (MRM) mode using negative electrospray ionization (ESI). The method was validated over the concentration range of 1.00-1000 ng/mL and 5.00-5000 ng/mL for OLM in human plasma and urine as well as 0.500-200 ng/mL and 25.0-25,000 ng/mL for HCTZ in human plasma and urine, respectively. Inter- and intra-run precision of OLM and HCTZ were less than 15% and the accuracy was within 85-115% for both plasma and urine. The average extraction recoveries were 96.6% and 92.7% for OLM, and 87.2% and 72.1% for HCTZ in human plasma and urine, respectively. The linearity, recovery, matrix effect and stability were validated for OLM/HCTZ in human plasma and urine.

Authors+Show Affiliations

Clinical Pharmacology Research Center, Peking Union Medical College Hospital and Chinese Academy of Medical Sciences, Beijing 100032, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20153270

Citation

Liu, Dongyang, et al. "Simultaneous Quantitative Determination of Olmesartan and Hydrochlorothiazide in Human Plasma and Urine By Liquid Chromatography Coupled to Tandem Mass Spectrometry." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 878, no. 9-10, 2010, pp. 743-8.
Liu D, Jiang J, Wang P, et al. Simultaneous quantitative determination of olmesartan and hydrochlorothiazide in human plasma and urine by liquid chromatography coupled to tandem mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2010;878(9-10):743-8.
Liu, D., Jiang, J., Wang, P., Feng, S., & Hu, P. (2010). Simultaneous quantitative determination of olmesartan and hydrochlorothiazide in human plasma and urine by liquid chromatography coupled to tandem mass spectrometry. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 878(9-10), 743-8. https://doi.org/10.1016/j.jchromb.2010.01.009
Liu D, et al. Simultaneous Quantitative Determination of Olmesartan and Hydrochlorothiazide in Human Plasma and Urine By Liquid Chromatography Coupled to Tandem Mass Spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2010 Mar 15;878(9-10):743-8. PubMed PMID: 20153270.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous quantitative determination of olmesartan and hydrochlorothiazide in human plasma and urine by liquid chromatography coupled to tandem mass spectrometry. AU - Liu,Dongyang, AU - Jiang,Ji, AU - Wang,Peng, AU - Feng,Sheng, AU - Hu,Pei, Y1 - 2010/01/18/ PY - 2009/04/29/received PY - 2010/01/07/revised PY - 2010/01/11/accepted PY - 2010/2/16/entrez PY - 2010/2/16/pubmed PY - 2010/5/15/medline SP - 743 EP - 8 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 878 IS - 9-10 N2 - A specific, sensitive and rapid method based on high performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS) was developed for the simultaneous determination of olmesartan (OLM) and hydrochlorothiazide (HCTZ) in human plasma and urine. Solid-phase extraction (SPE) was used to isolate the analytes from biological matrices followed by injection of the extracts onto a C(18) column with isocratic elution. Detection was carried out on a triple quadrupole tandem mass spectrometer in multiple reaction monitoring (MRM) mode using negative electrospray ionization (ESI). The method was validated over the concentration range of 1.00-1000 ng/mL and 5.00-5000 ng/mL for OLM in human plasma and urine as well as 0.500-200 ng/mL and 25.0-25,000 ng/mL for HCTZ in human plasma and urine, respectively. Inter- and intra-run precision of OLM and HCTZ were less than 15% and the accuracy was within 85-115% for both plasma and urine. The average extraction recoveries were 96.6% and 92.7% for OLM, and 87.2% and 72.1% for HCTZ in human plasma and urine, respectively. The linearity, recovery, matrix effect and stability were validated for OLM/HCTZ in human plasma and urine. SN - 1873-376X UR - https://www.unboundmedicine.com/medline/citation/20153270/Simultaneous_quantitative_determination_of_olmesartan_and_hydrochlorothiazide_in_human_plasma_and_urine_by_liquid_chromatography_coupled_to_tandem_mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(10)00021-8 DB - PRIME DP - Unbound Medicine ER -