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High sensitive detection of cancer cell with a folic acid-based boron-doped diamond electrode using an AC impedimetric approach.
Biosens Bioelectron. 2011 Jan 15; 26(5):1847-52.BB

Abstract

An electrochemical impedance method at a fixed frequency (10Hz) is used to detect folate receptor-rich cancer cells with the interaction between folic acid immobilized on gold nanoparticles deposited on boron-doped diamond (BDD) electrode surface and its receptor over-expressed on cancer cell membrane without additional labeling. Experimental results have shown that the BDD electrode modified with folic acid can clearly denote folate receptor-rich cancer cells, such as HeLa cells, at a concentration down to 10 cells mL(-1). The high sensitivity of BDD electrode is resulted from the change of field effect and surface charge density of BDD after cancer cells attachment. The BDD electrodes can be regenerated with 0.1M citric acid/glycine/HC1 at pH 3.0 and used seven times again. Therefore, electrochemical technique with BDD electrode has been introduced for cancer cells detection and a simple method to detect folate receptor-rich cancer cells without additional labeling has been developed.

Authors+Show Affiliations

Research Center of Biomedical Engineering, Department of Biomaterials, College of Materials, Xiamen University, 422, Siming South Road, Xiamen 361005, PR China. jweng@xmu.edu.cnNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20153626

Citation

Weng, Jian, et al. "High Sensitive Detection of Cancer Cell With a Folic Acid-based Boron-doped Diamond Electrode Using an AC Impedimetric Approach." Biosensors & Bioelectronics, vol. 26, no. 5, 2011, pp. 1847-52.
Weng J, Zhang Z, Sun L, et al. High sensitive detection of cancer cell with a folic acid-based boron-doped diamond electrode using an AC impedimetric approach. Biosens Bioelectron. 2011;26(5):1847-52.
Weng, J., Zhang, Z., Sun, L., & Wang, J. A. (2011). High sensitive detection of cancer cell with a folic acid-based boron-doped diamond electrode using an AC impedimetric approach. Biosensors & Bioelectronics, 26(5), 1847-52. https://doi.org/10.1016/j.bios.2010.01.027
Weng J, et al. High Sensitive Detection of Cancer Cell With a Folic Acid-based Boron-doped Diamond Electrode Using an AC Impedimetric Approach. Biosens Bioelectron. 2011 Jan 15;26(5):1847-52. PubMed PMID: 20153626.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High sensitive detection of cancer cell with a folic acid-based boron-doped diamond electrode using an AC impedimetric approach. AU - Weng,Jian, AU - Zhang,Zhaowu, AU - Sun,Liping, AU - Wang,Jin An, Y1 - 2010/02/01/ PY - 2009/11/18/received PY - 2010/01/22/revised PY - 2010/01/22/accepted PY - 2010/2/16/entrez PY - 2010/2/16/pubmed PY - 2011/4/16/medline SP - 1847 EP - 52 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 26 IS - 5 N2 - An electrochemical impedance method at a fixed frequency (10Hz) is used to detect folate receptor-rich cancer cells with the interaction between folic acid immobilized on gold nanoparticles deposited on boron-doped diamond (BDD) electrode surface and its receptor over-expressed on cancer cell membrane without additional labeling. Experimental results have shown that the BDD electrode modified with folic acid can clearly denote folate receptor-rich cancer cells, such as HeLa cells, at a concentration down to 10 cells mL(-1). The high sensitivity of BDD electrode is resulted from the change of field effect and surface charge density of BDD after cancer cells attachment. The BDD electrodes can be regenerated with 0.1M citric acid/glycine/HC1 at pH 3.0 and used seven times again. Therefore, electrochemical technique with BDD electrode has been introduced for cancer cells detection and a simple method to detect folate receptor-rich cancer cells without additional labeling has been developed. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/20153626/High_sensitive_detection_of_cancer_cell_with_a_folic_acid_based_boron_doped_diamond_electrode_using_an_AC_impedimetric_approach_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(10)00039-4 DB - PRIME DP - Unbound Medicine ER -