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Neuraminidase and hemagglutinin matching patterns of a highly pathogenic avian and two pandemic H1N1 influenza A viruses.
PLoS One 2010; 5(2):e9167Plos

Abstract

BACKGROUND

Influenza A virus displays strong reassortment characteristics, which enable it to achieve adaptation in human infection. Surveying the reassortment and virulence of novel viruses is important in the prevention and control of an influenza pandemic. Meanwhile, studying the mechanism of reassortment may accelerate the development of anti-influenza strategies.

METHODOLOGY/PRINCIPAL FINDINGS

The hemagglutinin (HA) and neuraminidase (NA) matching patterns of two pandemic H1N1 viruses (the 1918 and current 2009 strains) and a highly pathogenic avian influenza A virus (H5N1) were studied using a pseudotyped particle (pp) system. Our data showed that four of the six chimeric HA/NA combinations could produce infectious pps, and that some of the chimeric pps had greater infectivity than did their ancestors, raising the possibility of reassortment among these viruses. The NA of H5N1 (A/Anhui/1/2005) could hardly reassort with the HAs of the two H1N1 viruses. Many biological characteristics of HA and NA, including infectivity, hemagglutinating ability, and NA activity, are dependent on their matching pattern.

CONCLUSIONS/SIGNIFICANCE

Our data suggest the existence of an interaction between HA and NA, and the HA NA matching pattern is critical for valid viral reassortment.

Authors+Show Affiliations

Institute of Pathogens and Vectors, Dali University, Dali, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20161801

Citation

Zhang, Yonghui, et al. "Neuraminidase and Hemagglutinin Matching Patterns of a Highly Pathogenic Avian and Two Pandemic H1N1 Influenza a Viruses." PloS One, vol. 5, no. 2, 2010, pp. e9167.
Zhang Y, Lin X, Wang G, et al. Neuraminidase and hemagglutinin matching patterns of a highly pathogenic avian and two pandemic H1N1 influenza A viruses. PLoS ONE. 2010;5(2):e9167.
Zhang, Y., Lin, X., Wang, G., Zhou, J., Lu, J., Zhao, H., ... Wang, Y. (2010). Neuraminidase and hemagglutinin matching patterns of a highly pathogenic avian and two pandemic H1N1 influenza A viruses. PloS One, 5(2), pp. e9167. doi:10.1371/journal.pone.0009167.
Zhang Y, et al. Neuraminidase and Hemagglutinin Matching Patterns of a Highly Pathogenic Avian and Two Pandemic H1N1 Influenza a Viruses. PLoS ONE. 2010 Feb 11;5(2):e9167. PubMed PMID: 20161801.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Neuraminidase and hemagglutinin matching patterns of a highly pathogenic avian and two pandemic H1N1 influenza A viruses. AU - Zhang,Yonghui, AU - Lin,Xiaojing, AU - Wang,Guoqin, AU - Zhou,Jianfang, AU - Lu,Jian, AU - Zhao,Honglan, AU - Zhang,Fengwei, AU - Wu,Jia, AU - Xu,Chunqiong, AU - Du,Ning, AU - Li,Zi, AU - Zhang,Ye, AU - Wang,Xiaoyi, AU - Bi,Shengli, AU - Shu,Yuelong, AU - Zhou,Hongning, AU - Tan,Wenjie, AU - Wu,Xiaobing, AU - Chen,Zhihui, AU - Wang,Yue, Y1 - 2010/02/11/ PY - 2009/10/16/received PY - 2010/01/23/accepted PY - 2010/2/18/entrez PY - 2010/2/18/pubmed PY - 2010/10/1/medline SP - e9167 EP - e9167 JF - PloS one JO - PLoS ONE VL - 5 IS - 2 N2 - BACKGROUND: Influenza A virus displays strong reassortment characteristics, which enable it to achieve adaptation in human infection. Surveying the reassortment and virulence of novel viruses is important in the prevention and control of an influenza pandemic. Meanwhile, studying the mechanism of reassortment may accelerate the development of anti-influenza strategies. METHODOLOGY/PRINCIPAL FINDINGS: The hemagglutinin (HA) and neuraminidase (NA) matching patterns of two pandemic H1N1 viruses (the 1918 and current 2009 strains) and a highly pathogenic avian influenza A virus (H5N1) were studied using a pseudotyped particle (pp) system. Our data showed that four of the six chimeric HA/NA combinations could produce infectious pps, and that some of the chimeric pps had greater infectivity than did their ancestors, raising the possibility of reassortment among these viruses. The NA of H5N1 (A/Anhui/1/2005) could hardly reassort with the HAs of the two H1N1 viruses. Many biological characteristics of HA and NA, including infectivity, hemagglutinating ability, and NA activity, are dependent on their matching pattern. CONCLUSIONS/SIGNIFICANCE: Our data suggest the existence of an interaction between HA and NA, and the HA NA matching pattern is critical for valid viral reassortment. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/20161801/Neuraminidase_and_hemagglutinin_matching_patterns_of_a_highly_pathogenic_avian_and_two_pandemic_H1N1_influenza_A_viruses_ L2 - http://dx.plos.org/10.1371/journal.pone.0009167 DB - PRIME DP - Unbound Medicine ER -