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Simultaneous measurement of [15N]nitrate and [15N]nitrite enrichment and concentration in urine by gas chromatography mass spectrometry as pentafluorobenzyl derivatives.
Anal Chem. 2010 Mar 15; 82(6):2585-7.AC

Abstract

Quantification of (15)N-labeled nitrate and determination of the (15)N-enrichment in urine upon administration of (15)N-labeled precursors such as L-[guanidino-(15)N(2)]arginine is a suitable approach to study formation and metabolism of nitric oxide (NO) and its metabolites in vivo. Previously, we have reported on the simultaneous derivatization and accurate quantification of nitrite and nitrate in various biological fluids using pentafluorobenzyl bromide and GC/MS. We report here on a modification of this method that allows for the simultaneous determination of (15)N-enrichment of [(15)N]nitrate and [(15)N]nitrite and the simultaneous quantification of [(15)N]nitrate, [(14)N]nitrate, [(15)N]nitrite, and [(14)N]nitrite in human urine. In a pilot study, using the carbonic anhydrase inhibitor drug acetazolamide at therapeutical oral doses (5.4 and 5 mg per kg bodyweight) and by oral intake of [(15)N]nitrite (0.31 and 0.5 micromol per kg bodyweight) by two healthy volunteers, we demonstrate for the first time that renal carbonic anhydrase activity is mainly responsible for the reabsorption of nitrite from the primary urine and confirm previous findings on nitrate.

Authors+Show Affiliations

Institute of Clinical Pharmacology, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany. tsikas.dimitros@mh-hannover.deNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Validation Study

Language

eng

PubMed ID

20170083

Citation

Tsikas, Dimitrios, et al. "Simultaneous Measurement of [15N]nitrate and [15N]nitrite Enrichment and Concentration in Urine By Gas Chromatography Mass Spectrometry as Pentafluorobenzyl Derivatives." Analytical Chemistry, vol. 82, no. 6, 2010, pp. 2585-7.
Tsikas D, Schwarz A, Stichtenoth DO. Simultaneous measurement of [15N]nitrate and [15N]nitrite enrichment and concentration in urine by gas chromatography mass spectrometry as pentafluorobenzyl derivatives. Anal Chem. 2010;82(6):2585-7.
Tsikas, D., Schwarz, A., & Stichtenoth, D. O. (2010). Simultaneous measurement of [15N]nitrate and [15N]nitrite enrichment and concentration in urine by gas chromatography mass spectrometry as pentafluorobenzyl derivatives. Analytical Chemistry, 82(6), 2585-7. https://doi.org/10.1021/ac902970m
Tsikas D, Schwarz A, Stichtenoth DO. Simultaneous Measurement of [15N]nitrate and [15N]nitrite Enrichment and Concentration in Urine By Gas Chromatography Mass Spectrometry as Pentafluorobenzyl Derivatives. Anal Chem. 2010 Mar 15;82(6):2585-7. PubMed PMID: 20170083.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous measurement of [15N]nitrate and [15N]nitrite enrichment and concentration in urine by gas chromatography mass spectrometry as pentafluorobenzyl derivatives. AU - Tsikas,Dimitrios, AU - Schwarz,Alexandra, AU - Stichtenoth,Dirk O, PY - 2010/2/23/entrez PY - 2010/2/23/pubmed PY - 2010/6/9/medline SP - 2585 EP - 7 JF - Analytical chemistry JO - Anal. Chem. VL - 82 IS - 6 N2 - Quantification of (15)N-labeled nitrate and determination of the (15)N-enrichment in urine upon administration of (15)N-labeled precursors such as L-[guanidino-(15)N(2)]arginine is a suitable approach to study formation and metabolism of nitric oxide (NO) and its metabolites in vivo. Previously, we have reported on the simultaneous derivatization and accurate quantification of nitrite and nitrate in various biological fluids using pentafluorobenzyl bromide and GC/MS. We report here on a modification of this method that allows for the simultaneous determination of (15)N-enrichment of [(15)N]nitrate and [(15)N]nitrite and the simultaneous quantification of [(15)N]nitrate, [(14)N]nitrate, [(15)N]nitrite, and [(14)N]nitrite in human urine. In a pilot study, using the carbonic anhydrase inhibitor drug acetazolamide at therapeutical oral doses (5.4 and 5 mg per kg bodyweight) and by oral intake of [(15)N]nitrite (0.31 and 0.5 micromol per kg bodyweight) by two healthy volunteers, we demonstrate for the first time that renal carbonic anhydrase activity is mainly responsible for the reabsorption of nitrite from the primary urine and confirm previous findings on nitrate. SN - 1520-6882 UR - https://www.unboundmedicine.com/medline/citation/20170083/Simultaneous_measurement_of_[15N]nitrate_and_[15N]nitrite_enrichment_and_concentration_in_urine_by_gas_chromatography_mass_spectrometry_as_pentafluorobenzyl_derivatives_ L2 - https://dx.doi.org/10.1021/ac902970m DB - PRIME DP - Unbound Medicine ER -