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GM1 and nerve growth factor modulate mitochondrial membrane potential and neurofilament light mRNA expression in cultured dorsal root ganglion and spinal cord neurons during excitotoxic glutamate exposure.
J Clin Neurosci. 2010 Apr; 17(4):495-500.JC

Abstract

Monosialoganglioside GM1 is a known neurotrophic factor. Nerve growth factor (NGF), a member of the neurotrophin family, is important for the survival, differentiation and maturation of neurons. The aim of this study was to test whether administration of GM1 and NGF can ameliorate glutamate (Glu) neurotoxicity in primary cultured embryonic rat dorsal root ganglia (DRG) and spinal cord neurons, and to investigate the mechanism underlying any effect. DRG and spinal cord neurons were exposed to the following treatments: Glu (2 mmol/L); Glu (2 mmol/L) plus GM1 (10mg/mL); Glu (2 mmol/l) plus NGF (10 ng/mL); Glu (2 mmol/L) plus GM1 (5mg/mL) and NGF (5 ng/mL). Cell viability was assessed using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, ultrastructural alterations were examined using inverse phase contrast microscopy and electron microscopy, mitochondrial membrane potential was measured using rhodamine 123 labeling and flow cytometry, and neurofilament light (NF-L) mRNA expression was detected by reverse transcription-polymerase chain reaction. It was found that GM1 and NGF can increase the viability of neurons incubated with Glu, which, after GM1 and NGF treatment, were almost morphologically normal. The mitochondrial membrane potential of neurons was lowest for neurons treated with Glu alone, and that for neurons treated with Glu plus GM1 and NGF was higher than that for treatment with GM1 or NGF alone. The mRNA of NF-L was expressed at the highest level in neurons treated with Glu plus GM1 and NGF. Our findings indicate that NGF and GM1 act synergistically to protect DRG and spinal cord neurons from Glu cytotoxicity. NGF and GM1 may function by maintaining normal mitochondrial membrane potential or by promoting NF-L mRNA expression.

Authors+Show Affiliations

Department of Human Anatomy, Binzhou Medical College, 346 Guanhai Road, Laishan, Yantai 264003, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20171893

Citation

Huang, Fei, et al. "GM1 and Nerve Growth Factor Modulate Mitochondrial Membrane Potential and Neurofilament Light mRNA Expression in Cultured Dorsal Root Ganglion and Spinal Cord Neurons During Excitotoxic Glutamate Exposure." Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia, vol. 17, no. 4, 2010, pp. 495-500.
Huang F, Dong X, Zhang L, et al. GM1 and nerve growth factor modulate mitochondrial membrane potential and neurofilament light mRNA expression in cultured dorsal root ganglion and spinal cord neurons during excitotoxic glutamate exposure. J Clin Neurosci. 2010;17(4):495-500.
Huang, F., Dong, X., Zhang, L., Zhang, X., Zhao, D., Bai, X., & Li, Z. (2010). GM1 and nerve growth factor modulate mitochondrial membrane potential and neurofilament light mRNA expression in cultured dorsal root ganglion and spinal cord neurons during excitotoxic glutamate exposure. Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia, 17(4), 495-500. https://doi.org/10.1016/j.jocn.2009.07.112
Huang F, et al. GM1 and Nerve Growth Factor Modulate Mitochondrial Membrane Potential and Neurofilament Light mRNA Expression in Cultured Dorsal Root Ganglion and Spinal Cord Neurons During Excitotoxic Glutamate Exposure. J Clin Neurosci. 2010;17(4):495-500. PubMed PMID: 20171893.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - GM1 and nerve growth factor modulate mitochondrial membrane potential and neurofilament light mRNA expression in cultured dorsal root ganglion and spinal cord neurons during excitotoxic glutamate exposure. AU - Huang,Fei, AU - Dong,Xifeng, AU - Zhang,Luping, AU - Zhang,Xuejun, AU - Zhao,Dongmei, AU - Bai,Xianyong, AU - Li,Zhenzhong, Y1 - 2010/02/19/ PY - 2008/11/11/received PY - 2009/07/17/revised PY - 2009/07/23/accepted PY - 2010/2/23/entrez PY - 2010/2/23/pubmed PY - 2010/6/15/medline SP - 495 EP - 500 JF - Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia JO - J Clin Neurosci VL - 17 IS - 4 N2 - Monosialoganglioside GM1 is a known neurotrophic factor. Nerve growth factor (NGF), a member of the neurotrophin family, is important for the survival, differentiation and maturation of neurons. The aim of this study was to test whether administration of GM1 and NGF can ameliorate glutamate (Glu) neurotoxicity in primary cultured embryonic rat dorsal root ganglia (DRG) and spinal cord neurons, and to investigate the mechanism underlying any effect. DRG and spinal cord neurons were exposed to the following treatments: Glu (2 mmol/L); Glu (2 mmol/L) plus GM1 (10mg/mL); Glu (2 mmol/l) plus NGF (10 ng/mL); Glu (2 mmol/L) plus GM1 (5mg/mL) and NGF (5 ng/mL). Cell viability was assessed using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, ultrastructural alterations were examined using inverse phase contrast microscopy and electron microscopy, mitochondrial membrane potential was measured using rhodamine 123 labeling and flow cytometry, and neurofilament light (NF-L) mRNA expression was detected by reverse transcription-polymerase chain reaction. It was found that GM1 and NGF can increase the viability of neurons incubated with Glu, which, after GM1 and NGF treatment, were almost morphologically normal. The mitochondrial membrane potential of neurons was lowest for neurons treated with Glu alone, and that for neurons treated with Glu plus GM1 and NGF was higher than that for treatment with GM1 or NGF alone. The mRNA of NF-L was expressed at the highest level in neurons treated with Glu plus GM1 and NGF. Our findings indicate that NGF and GM1 act synergistically to protect DRG and spinal cord neurons from Glu cytotoxicity. NGF and GM1 may function by maintaining normal mitochondrial membrane potential or by promoting NF-L mRNA expression. SN - 1532-2653 UR - https://www.unboundmedicine.com/medline/citation/20171893/GM1_and_nerve_growth_factor_modulate_mitochondrial_membrane_potential_and_neurofilament_light_mRNA_expression_in_cultured_dorsal_root_ganglion_and_spinal_cord_neurons_during_excitotoxic_glutamate_exposure_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0967-5868(09)00626-2 DB - PRIME DP - Unbound Medicine ER -