Tags

Type your tag names separated by a space and hit enter

Diagnostic performance of IgG anti-deamidated gliadin peptide antibody assays is comparable to IgA anti-tTG in celiac disease.
Clin Chim Acta 2010; 411(13-14):931-5CC

Abstract

BACKGROUND

Detection of IgG antibodies against deamidated gliadin peptides (DGP) is more sensitive and more specific for celiac disease than detection of IgG antibodies against native gliadin. Our aim was to evaluate the technical performance and diagnostic accuracy of four commercial IgG anti-DGP assays.

METHODS

Commercial IgG anti-DGP assays from Euroimmun, Inova, Phadia and The Binding Site were evaluated and their diagnostic accuracy (sensitivity and specificity) compared to other serologic assays for celiac disease (3IgA and 2IgG anti-tTG assays, 1IgA and 1IgG anti-gliadin assay, 1IgA anti-DGP assay). The study population consisted of 86 consecutive CD patients and 741 disease controls.

RESULTS

The technical performance (linearity, interference and imprecision) of the IgG anti-DGP assays was acceptable. The sensitivity of the IgG anti-DGP assays varied between 76.7% and 86.0% at the cut-off recommended by the manufacturer and between 74.4% and 86.0% at the cut-off that corresponded to a specificity of 98%. The specificity varied between 97.3% and 99.3%. The diagnostic accuracy of the IgG anti-DGP assays was comparable to the diagnostic accuracy of the IgA anti-tTG assays. The sensitivity of the IgG anti-DGP assays was significantly better than sensitivity of the IgG anti-tTG assays (p<0.05) and the specificity was significantly better than the IgA and IgG anti-gliadin assays (p<0.05).

CONCLUSIONS

The overall performance of the four IgG anti-DGP assays was acceptable and the diagnostic accuracy comparable to the three IgA anti-tTG assays.

Authors+Show Affiliations

Laboratory Medicine, Immunology, University Hospitals Leuven, Catholic University of Leuven, Leuven, Belgium.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

20171961

Citation

Vermeersch, Pieter, et al. "Diagnostic Performance of IgG Anti-deamidated Gliadin Peptide Antibody Assays Is Comparable to IgA anti-tTG in Celiac Disease." Clinica Chimica Acta; International Journal of Clinical Chemistry, vol. 411, no. 13-14, 2010, pp. 931-5.
Vermeersch P, Geboes K, Mariën G, et al. Diagnostic performance of IgG anti-deamidated gliadin peptide antibody assays is comparable to IgA anti-tTG in celiac disease. Clin Chim Acta. 2010;411(13-14):931-5.
Vermeersch, P., Geboes, K., Mariën, G., Hoffman, I., Hiele, M., & Bossuyt, X. (2010). Diagnostic performance of IgG anti-deamidated gliadin peptide antibody assays is comparable to IgA anti-tTG in celiac disease. Clinica Chimica Acta; International Journal of Clinical Chemistry, 411(13-14), pp. 931-5. doi:10.1016/j.cca.2010.02.060.
Vermeersch P, et al. Diagnostic Performance of IgG Anti-deamidated Gliadin Peptide Antibody Assays Is Comparable to IgA anti-tTG in Celiac Disease. Clin Chim Acta. 2010 Jul 4;411(13-14):931-5. PubMed PMID: 20171961.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diagnostic performance of IgG anti-deamidated gliadin peptide antibody assays is comparable to IgA anti-tTG in celiac disease. AU - Vermeersch,Pieter, AU - Geboes,Karel, AU - Mariën,Godelieve, AU - Hoffman,Ilse, AU - Hiele,Martin, AU - Bossuyt,Xavier, Y1 - 2010/02/19/ PY - 2010/01/19/received PY - 2010/02/16/revised PY - 2010/02/16/accepted PY - 2010/2/23/entrez PY - 2010/2/23/pubmed PY - 2010/8/31/medline SP - 931 EP - 5 JF - Clinica chimica acta; international journal of clinical chemistry JO - Clin. Chim. Acta VL - 411 IS - 13-14 N2 - BACKGROUND: Detection of IgG antibodies against deamidated gliadin peptides (DGP) is more sensitive and more specific for celiac disease than detection of IgG antibodies against native gliadin. Our aim was to evaluate the technical performance and diagnostic accuracy of four commercial IgG anti-DGP assays. METHODS: Commercial IgG anti-DGP assays from Euroimmun, Inova, Phadia and The Binding Site were evaluated and their diagnostic accuracy (sensitivity and specificity) compared to other serologic assays for celiac disease (3IgA and 2IgG anti-tTG assays, 1IgA and 1IgG anti-gliadin assay, 1IgA anti-DGP assay). The study population consisted of 86 consecutive CD patients and 741 disease controls. RESULTS: The technical performance (linearity, interference and imprecision) of the IgG anti-DGP assays was acceptable. The sensitivity of the IgG anti-DGP assays varied between 76.7% and 86.0% at the cut-off recommended by the manufacturer and between 74.4% and 86.0% at the cut-off that corresponded to a specificity of 98%. The specificity varied between 97.3% and 99.3%. The diagnostic accuracy of the IgG anti-DGP assays was comparable to the diagnostic accuracy of the IgA anti-tTG assays. The sensitivity of the IgG anti-DGP assays was significantly better than sensitivity of the IgG anti-tTG assays (p<0.05) and the specificity was significantly better than the IgA and IgG anti-gliadin assays (p<0.05). CONCLUSIONS: The overall performance of the four IgG anti-DGP assays was acceptable and the diagnostic accuracy comparable to the three IgA anti-tTG assays. SN - 1873-3492 UR - https://www.unboundmedicine.com/medline/citation/20171961/Diagnostic_performance_of_IgG_anti_deamidated_gliadin_peptide_antibody_assays_is_comparable_to_IgA_anti_tTG_in_celiac_disease_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0009-8981(10)00155-5 DB - PRIME DP - Unbound Medicine ER -