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Dendritic cells and B cells cooperate in the generation of CD4(+)CD25(+)FOXP3(+) allogeneic T cells.
Transplant Proc. 2010 Jan-Feb; 42(1):371-5.TP

Abstract

BACKGROUND

CD4(+)CD25(+)Foxp3(+) regulatory T cells (Treg) play an essential role in immune tolerance, suppressing responses against self-antigens. Additionally, Treg play an important role in maintaining immunosuppression to alloantigens as well as to other antigens. It is well known that in the gut, a subset of dendritic cells produces retinoic acid (RA), which together with transforming growth factor (TGF-beta) is able to differentiate naïve T cells into Treg. The aim of this study was to establish the role of antigen-presenting cells (APC) in the differentiation of allogeneic Tregs under the effect of RA and TGF-beta.

METHODS

Splenic CD4(+)CD25(-) naïve T cells from C57BL/6 mice were co-cultured with splenic CD11c-enriched APC from Balb/c mice in the presence of TGF-beta, RA, and interleukin (IL-2). After 6 days of culture, cells were analyzed for the expression of Foxp3 by flow cytometry. Additionally, we investigated the role of B cells and dendritic cells (DCs) and their stimulatory capacity in the generation of Tregs.

RESULTS

Our results showed that co-culture of naive T cells with the appropriate level of stimulation by APC in the presence of TGF-beta, RA, and IL-2 provided a new powerful approach to generate allogeneic Treg cells. We demonstrated that although B cells and DCs can generate Tregs by themselves, a mixure of both APC improved their capacity to efficiently generate Tregs. Also, we observed that although the addition of IL-2 to the cultures was not crucial to generate Tregs, it was required to optimize their expansion and cell survival.

Authors+Show Affiliations

Biology Department, Universidad de Chile, Santiago, Chile.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20172352

Citation

Moore, C, et al. "Dendritic Cells and B Cells Cooperate in the Generation of CD4(+)CD25(+)FOXP3(+) Allogeneic T Cells." Transplantation Proceedings, vol. 42, no. 1, 2010, pp. 371-5.
Moore C, Sauma D, Reyes PA, et al. Dendritic cells and B cells cooperate in the generation of CD4(+)CD25(+)FOXP3(+) allogeneic T cells. Transplant Proc. 2010;42(1):371-5.
Moore, C., Sauma, D., Reyes, P. A., Morales, J., Rosemblatt, M., Bono, M. R., & Fierro, J. A. (2010). Dendritic cells and B cells cooperate in the generation of CD4(+)CD25(+)FOXP3(+) allogeneic T cells. Transplantation Proceedings, 42(1), 371-5. https://doi.org/10.1016/j.transproceed.2009.12.044
Moore C, et al. Dendritic Cells and B Cells Cooperate in the Generation of CD4(+)CD25(+)FOXP3(+) Allogeneic T Cells. Transplant Proc. 2010;42(1):371-5. PubMed PMID: 20172352.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dendritic cells and B cells cooperate in the generation of CD4(+)CD25(+)FOXP3(+) allogeneic T cells. AU - Moore,C, AU - Sauma,D, AU - Reyes,P A, AU - Morales,J, AU - Rosemblatt,M, AU - Bono,M R, AU - Fierro,J A, PY - 2010/2/23/entrez PY - 2010/2/23/pubmed PY - 2010/6/4/medline SP - 371 EP - 5 JF - Transplantation proceedings JO - Transplant. Proc. VL - 42 IS - 1 N2 - BACKGROUND: CD4(+)CD25(+)Foxp3(+) regulatory T cells (Treg) play an essential role in immune tolerance, suppressing responses against self-antigens. Additionally, Treg play an important role in maintaining immunosuppression to alloantigens as well as to other antigens. It is well known that in the gut, a subset of dendritic cells produces retinoic acid (RA), which together with transforming growth factor (TGF-beta) is able to differentiate naïve T cells into Treg. The aim of this study was to establish the role of antigen-presenting cells (APC) in the differentiation of allogeneic Tregs under the effect of RA and TGF-beta. METHODS: Splenic CD4(+)CD25(-) naïve T cells from C57BL/6 mice were co-cultured with splenic CD11c-enriched APC from Balb/c mice in the presence of TGF-beta, RA, and interleukin (IL-2). After 6 days of culture, cells were analyzed for the expression of Foxp3 by flow cytometry. Additionally, we investigated the role of B cells and dendritic cells (DCs) and their stimulatory capacity in the generation of Tregs. RESULTS: Our results showed that co-culture of naive T cells with the appropriate level of stimulation by APC in the presence of TGF-beta, RA, and IL-2 provided a new powerful approach to generate allogeneic Treg cells. We demonstrated that although B cells and DCs can generate Tregs by themselves, a mixure of both APC improved their capacity to efficiently generate Tregs. Also, we observed that although the addition of IL-2 to the cultures was not crucial to generate Tregs, it was required to optimize their expansion and cell survival. SN - 1873-2623 UR - https://www.unboundmedicine.com/medline/citation/20172352/Dendritic_cells_and_B_cells_cooperate_in_the_generation_of_CD4_+_CD25_+_FOXP3_+__allogeneic_T_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0041-1345(09)01800-4 DB - PRIME DP - Unbound Medicine ER -