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Vitamin D receptor and vitamin D metabolizing enzymes are expressed in the human male reproductive tract.
Hum Reprod. 2010 May; 25(5):1303-11.HR

Abstract

BACKGROUND

The vitamin D receptor (VDR) is expressed in human testis, and vitamin D (VD) has been suggested to affect survival and function of mature spermatozoa. Indeed, VDR knockout mice and VD deficient rats show decreased sperm counts and low fertility. However, the cellular response to VD is complex, since it is not solely dependent on VDR expression, but also on cellular uptake of circulating VD and presence and activity of VD metabolizing enzymes. Expression of VD metabolizing enzymes has not previously been investigated in human testis and male reproductive tract. Therefore, we performed a comprehensive analysis of the expression of VDR, VD activating (CYP2R1, CYP27A1, CYP27B1) and inactivating (CYP24A1) enzymes in the testis, epididymis, seminal vesicle (SV), prostate and spermatozoa.

METHODS

Tissue samples were obtained after orchiectomy (testis n = 13; epididymis n = 7), prostatectomy (prostate n = 5 and SVs n = 3) and semen samples obtained after ejaculation (n = 13). mRNA was detected with RT-PCR and expression of proteins was determined by immunohistochemistry.

RESULTS

VDR and VD metabolizing enzymes were concomitantly expressed in round and elongated spermatids, vesicles within the caput epididymis, and glandular epithelium of cauda epididymis, SV and prostate. The expression pattern in ejaculated spermatozoa varied, although, concomitant expression of VDR, CYP2R1, CYP27B1 and CYP24A1 was observed in neck and midpiece in a subpopulation of mature spermatozoa.

CONCLUSION

On the basis of the marked expression of VDR and the VD metabolizing enzymes in human testis, ejaculatory tract and mature spermatozoa, we suggest that VD is important for spermatogenesis and maturation of human spermatozoa.

Authors+Show Affiliations

University Department of Growth and Reproduction GR, Rigshospitalet section 5064, Blegdamsvej 9, DK-2100 Copenhagen, Denmark. mabj@rh.regionh.dkNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20172873

Citation

Blomberg Jensen, Martin, et al. "Vitamin D Receptor and Vitamin D Metabolizing Enzymes Are Expressed in the Human Male Reproductive Tract." Human Reproduction (Oxford, England), vol. 25, no. 5, 2010, pp. 1303-11.
Blomberg Jensen M, Nielsen JE, Jørgensen A, et al. Vitamin D receptor and vitamin D metabolizing enzymes are expressed in the human male reproductive tract. Hum Reprod. 2010;25(5):1303-11.
Blomberg Jensen, M., Nielsen, J. E., Jørgensen, A., Rajpert-De Meyts, E., Kristensen, D. M., Jørgensen, N., Skakkebaek, N. E., Juul, A., & Leffers, H. (2010). Vitamin D receptor and vitamin D metabolizing enzymes are expressed in the human male reproductive tract. Human Reproduction (Oxford, England), 25(5), 1303-11. https://doi.org/10.1093/humrep/deq024
Blomberg Jensen M, et al. Vitamin D Receptor and Vitamin D Metabolizing Enzymes Are Expressed in the Human Male Reproductive Tract. Hum Reprod. 2010;25(5):1303-11. PubMed PMID: 20172873.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Vitamin D receptor and vitamin D metabolizing enzymes are expressed in the human male reproductive tract. AU - Blomberg Jensen,Martin, AU - Nielsen,John E, AU - Jørgensen,Anne, AU - Rajpert-De Meyts,Ewa, AU - Kristensen,David Møbjerg, AU - Jørgensen,Niels, AU - Skakkebaek,Niels E, AU - Juul,Anders, AU - Leffers,Henrik, Y1 - 2010/02/18/ PY - 2010/2/23/entrez PY - 2010/2/23/pubmed PY - 2010/7/30/medline SP - 1303 EP - 11 JF - Human reproduction (Oxford, England) JO - Hum. Reprod. VL - 25 IS - 5 N2 - BACKGROUND: The vitamin D receptor (VDR) is expressed in human testis, and vitamin D (VD) has been suggested to affect survival and function of mature spermatozoa. Indeed, VDR knockout mice and VD deficient rats show decreased sperm counts and low fertility. However, the cellular response to VD is complex, since it is not solely dependent on VDR expression, but also on cellular uptake of circulating VD and presence and activity of VD metabolizing enzymes. Expression of VD metabolizing enzymes has not previously been investigated in human testis and male reproductive tract. Therefore, we performed a comprehensive analysis of the expression of VDR, VD activating (CYP2R1, CYP27A1, CYP27B1) and inactivating (CYP24A1) enzymes in the testis, epididymis, seminal vesicle (SV), prostate and spermatozoa. METHODS: Tissue samples were obtained after orchiectomy (testis n = 13; epididymis n = 7), prostatectomy (prostate n = 5 and SVs n = 3) and semen samples obtained after ejaculation (n = 13). mRNA was detected with RT-PCR and expression of proteins was determined by immunohistochemistry. RESULTS: VDR and VD metabolizing enzymes were concomitantly expressed in round and elongated spermatids, vesicles within the caput epididymis, and glandular epithelium of cauda epididymis, SV and prostate. The expression pattern in ejaculated spermatozoa varied, although, concomitant expression of VDR, CYP2R1, CYP27B1 and CYP24A1 was observed in neck and midpiece in a subpopulation of mature spermatozoa. CONCLUSION: On the basis of the marked expression of VDR and the VD metabolizing enzymes in human testis, ejaculatory tract and mature spermatozoa, we suggest that VD is important for spermatogenesis and maturation of human spermatozoa. SN - 1460-2350 UR - https://www.unboundmedicine.com/medline/citation/20172873/Vitamin_D_receptor_and_vitamin_D_metabolizing_enzymes_are_expressed_in_the_human_male_reproductive_tract_ L2 - https://academic.oup.com/humrep/article-lookup/doi/10.1093/humrep/deq024 DB - PRIME DP - Unbound Medicine ER -