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Tea catechins reduce inflammatory reactions via mitogen-activated protein kinase pathways in toll-like receptor 2 ligand-stimulated dental pulp cells.
Life Sci 2010; 86(17-18):654-60LS

Abstract

AIMS

In this study, we evaluated whether catechins could inhibit the expression of pro-inflammatory mediators induced by dental caries-related bacteria, Streptococci, or pathogen-associated molecular patterns (PAMPs) stimulation in human dental pulp fibroblasts (HDPF). We further determined the mechanisms of the anti-inflammatory activity of catechins.

MAIN METHODS

Streptococci or PAMP-stimulated HDPF were treated with catechin, and then the expression and production of pro-inflammatory mediators were determined by RT-PCR and ELISA. Furthermore, the signal transduction pathways activated with toll-like receptor (TLR)2 ligand were assessed by Immunoblot and ELISA using blocking assay with specific inhibitors.

KEY FINDINGS

Increased expressions of pro-inflammatory mediators are found in inflamed dental pulp, especially in HDPF. We recently reported that dental pulpal innate immune responses may mainly result from the predominantly-expressed TLR2 signaling. Catechins, polyphenolic compounds in green tea, exert protective and healing effects through multiple mechanisms, including antioxidative and anti-inflammatory effects. However, there are no reports concerning the effects of catechins on dental pulp. In this study, we demonstrated that the up-regulated expressions of IL-8 or PGE(2) in Streptococci or PAMP-stimulated HDPF were inhibited by catechins, (-)-epicatechin gallate (ECG) and (-)-epigallocatechin gallate (EGCG). In TLR2 ligand-stimulated HDPF, specific inhibitors of extracellular signal regulated kinase (ERK)1/2, p38, c-jun NH(2)-terminal kinase (SAP/JNK), NF-kappaB or catechins markedly reduced the level of pro-inflammatory mediators and the phosphorylation of these signal transduction molecules was suppressed by catechins.

SIGNIFICANCE

These findings suggest that catechins might be useful therapeutically as an anti-inflammatory modulator of dental pulpal inflammation.

Authors+Show Affiliations

Department of Conservative Dentistry, Institute of Health Biosciences, The University of Tokushima Graduate School, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20176036

Citation

Hirao, Kouji, et al. "Tea Catechins Reduce Inflammatory Reactions Via Mitogen-activated Protein Kinase Pathways in Toll-like Receptor 2 Ligand-stimulated Dental Pulp Cells." Life Sciences, vol. 86, no. 17-18, 2010, pp. 654-60.
Hirao K, Yumoto H, Nakanishi T, et al. Tea catechins reduce inflammatory reactions via mitogen-activated protein kinase pathways in toll-like receptor 2 ligand-stimulated dental pulp cells. Life Sci. 2010;86(17-18):654-60.
Hirao, K., Yumoto, H., Nakanishi, T., Mukai, K., Takahashi, K., Takegawa, D., & Matsuo, T. (2010). Tea catechins reduce inflammatory reactions via mitogen-activated protein kinase pathways in toll-like receptor 2 ligand-stimulated dental pulp cells. Life Sciences, 86(17-18), pp. 654-60. doi:10.1016/j.lfs.2010.02.017.
Hirao K, et al. Tea Catechins Reduce Inflammatory Reactions Via Mitogen-activated Protein Kinase Pathways in Toll-like Receptor 2 Ligand-stimulated Dental Pulp Cells. Life Sci. 2010 Apr 24;86(17-18):654-60. PubMed PMID: 20176036.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Tea catechins reduce inflammatory reactions via mitogen-activated protein kinase pathways in toll-like receptor 2 ligand-stimulated dental pulp cells. AU - Hirao,Kouji, AU - Yumoto,Hiromichi, AU - Nakanishi,Tadashi, AU - Mukai,Kayo, AU - Takahashi,Kanako, AU - Takegawa,Daisuke, AU - Matsuo,Takashi, Y1 - 2010/02/20/ PY - 2009/11/04/received PY - 2010/02/11/revised PY - 2010/02/12/accepted PY - 2010/2/24/entrez PY - 2010/2/24/pubmed PY - 2010/4/22/medline SP - 654 EP - 60 JF - Life sciences JO - Life Sci. VL - 86 IS - 17-18 N2 - AIMS: In this study, we evaluated whether catechins could inhibit the expression of pro-inflammatory mediators induced by dental caries-related bacteria, Streptococci, or pathogen-associated molecular patterns (PAMPs) stimulation in human dental pulp fibroblasts (HDPF). We further determined the mechanisms of the anti-inflammatory activity of catechins. MAIN METHODS: Streptococci or PAMP-stimulated HDPF were treated with catechin, and then the expression and production of pro-inflammatory mediators were determined by RT-PCR and ELISA. Furthermore, the signal transduction pathways activated with toll-like receptor (TLR)2 ligand were assessed by Immunoblot and ELISA using blocking assay with specific inhibitors. KEY FINDINGS: Increased expressions of pro-inflammatory mediators are found in inflamed dental pulp, especially in HDPF. We recently reported that dental pulpal innate immune responses may mainly result from the predominantly-expressed TLR2 signaling. Catechins, polyphenolic compounds in green tea, exert protective and healing effects through multiple mechanisms, including antioxidative and anti-inflammatory effects. However, there are no reports concerning the effects of catechins on dental pulp. In this study, we demonstrated that the up-regulated expressions of IL-8 or PGE(2) in Streptococci or PAMP-stimulated HDPF were inhibited by catechins, (-)-epicatechin gallate (ECG) and (-)-epigallocatechin gallate (EGCG). In TLR2 ligand-stimulated HDPF, specific inhibitors of extracellular signal regulated kinase (ERK)1/2, p38, c-jun NH(2)-terminal kinase (SAP/JNK), NF-kappaB or catechins markedly reduced the level of pro-inflammatory mediators and the phosphorylation of these signal transduction molecules was suppressed by catechins. SIGNIFICANCE: These findings suggest that catechins might be useful therapeutically as an anti-inflammatory modulator of dental pulpal inflammation. SN - 1879-0631 UR - https://www.unboundmedicine.com/medline/citation/20176036/Tea_catechins_reduce_inflammatory_reactions_via_mitogen_activated_protein_kinase_pathways_in_toll_like_receptor_2_ligand_stimulated_dental_pulp_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0024-3205(10)00078-0 DB - PRIME DP - Unbound Medicine ER -