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Effects of influenza immunization on humoral and cellular alloreactivity in humans.
Transplantation 2010; 89(7):838-44T

Abstract

BACKGROUND

Alloreactive T cells and anti-human leukocyte antigen antibodies mediate transplant injury. Environmental exposures, including vaccinations, may activate the alloimmune repertoire leading to accelerated allograft injury. To test whether vaccination impacts human alloimmunity, we analyzed humoral and cellular immune reactivity in subjects undergoing influenza vaccination.

METHODS

We serially obtained blood samples from 30 healthy subjects and 8 kidney and 9 lung transplant recipients who received influenza vaccination, and from 20 healthy unvaccinated controls. We measured cellular and humoral anti-influenza responses, anti-human leukocyte antigen antibodies, and alloreactive T-cell immunity (interferon-gamma ELISPOT) at 0, 2, 4, and 12 weeks after vaccination.

RESULTS

Vaccination induced influenza-reactive humoral and cellular responses in control subjects and in transplant recipients. Only two of 30 vaccinated volunteers developed new alloantibodies, but none of the transplant patients. Vaccination also specifically and significantly augmented cellular alloimmunity based on reactivity to a panel of stimulators in both healthy subjects and in transplant recipients within 4 weeks of vaccination. The enhanced cellular alloresponse waned toward prevaccine levels by week 12.

CONCLUSION

Our findings newly demonstrate that influenza vaccination can have a significant impact on the potency of the alloimmune repertoire. Because the strength of the alloresponse influences long-term graft function, our results suggest that further investigation of alloimmune monitoring after vaccination is needed.

Authors+Show Affiliations

Department of Pediatrics, Cleveland Clinic, Cleveland, OH, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

20179666

Citation

Danziger-Isakov, Lara, et al. "Effects of Influenza Immunization On Humoral and Cellular Alloreactivity in Humans." Transplantation, vol. 89, no. 7, 2010, pp. 838-44.
Danziger-Isakov L, Cherkassky L, Siegel H, et al. Effects of influenza immunization on humoral and cellular alloreactivity in humans. Transplantation. 2010;89(7):838-44.
Danziger-Isakov, L., Cherkassky, L., Siegel, H., McManamon, M., Kramer, K., Budev, M., ... Poggio, E. D. (2010). Effects of influenza immunization on humoral and cellular alloreactivity in humans. Transplantation, 89(7), pp. 838-44. doi:10.1097/TP.0b013e3181ca56f8.
Danziger-Isakov L, et al. Effects of Influenza Immunization On Humoral and Cellular Alloreactivity in Humans. Transplantation. 2010 Apr 15;89(7):838-44. PubMed PMID: 20179666.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of influenza immunization on humoral and cellular alloreactivity in humans. AU - Danziger-Isakov,Lara, AU - Cherkassky,Leonid, AU - Siegel,Hanni, AU - McManamon,Mary, AU - Kramer,Kristen, AU - Budev,Marie, AU - Sawinski,Deirdre, AU - Augustine,Joshua J, AU - Hricik,Donald E, AU - Fairchild,Robert, AU - Heeger,Peter S, AU - Poggio,Emilio D, PY - 2010/2/25/entrez PY - 2010/2/25/pubmed PY - 2010/4/30/medline SP - 838 EP - 44 JF - Transplantation JO - Transplantation VL - 89 IS - 7 N2 - BACKGROUND: Alloreactive T cells and anti-human leukocyte antigen antibodies mediate transplant injury. Environmental exposures, including vaccinations, may activate the alloimmune repertoire leading to accelerated allograft injury. To test whether vaccination impacts human alloimmunity, we analyzed humoral and cellular immune reactivity in subjects undergoing influenza vaccination. METHODS: We serially obtained blood samples from 30 healthy subjects and 8 kidney and 9 lung transplant recipients who received influenza vaccination, and from 20 healthy unvaccinated controls. We measured cellular and humoral anti-influenza responses, anti-human leukocyte antigen antibodies, and alloreactive T-cell immunity (interferon-gamma ELISPOT) at 0, 2, 4, and 12 weeks after vaccination. RESULTS: Vaccination induced influenza-reactive humoral and cellular responses in control subjects and in transplant recipients. Only two of 30 vaccinated volunteers developed new alloantibodies, but none of the transplant patients. Vaccination also specifically and significantly augmented cellular alloimmunity based on reactivity to a panel of stimulators in both healthy subjects and in transplant recipients within 4 weeks of vaccination. The enhanced cellular alloresponse waned toward prevaccine levels by week 12. CONCLUSION: Our findings newly demonstrate that influenza vaccination can have a significant impact on the potency of the alloimmune repertoire. Because the strength of the alloresponse influences long-term graft function, our results suggest that further investigation of alloimmune monitoring after vaccination is needed. SN - 1534-6080 UR - https://www.unboundmedicine.com/medline/citation/20179666/Effects_of_influenza_immunization_on_humoral_and_cellular_alloreactivity_in_humans_ L2 - http://Insights.ovid.com/pubmed?pmid=20179666 DB - PRIME DP - Unbound Medicine ER -