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A photoelectrochemical sensor based on CdS-polyamidoamine nano-composite film for cell capture and detection.
Biosens Bioelectron. 2010 May 15; 25(9):2045-50.BB

Abstract

We demonstrated herein a newly developed photoelectrochemical cell-sensor for the determination of SMMC-7721 human hepatoma carcinoma cells (SMMC-7721 cells) by using a photosensitive CdS-polyamidoamine (G4) nano-composite film (CdS-PAMAM). The film was generated by electrodeposition method. The presence of PAMAM in the film eliminated the surface defects of CdS nanoparticles and therefore resulted in a greatly enhanced photocurrent and a reduced dark current. In the presence of the electron donor ascorbic acid (AA), the photoexcitation of this modified electrode potentiostated at 0 V versus Ag/AgCl led to an anodic photocurrent. As a result of the covalent coupling reactions, a layer of concanavalin A (ConA) was firmly bound to the functionalized CdS-PAMAM film via glutaraldehyde bridges. The resulting modified electrodes were tested as sensors for SMMC-7721 cell capture and detection via affinity interactions between ConA and mannosyl groups on cell surface. The cell concentration was measured from 5.0 x 10(3) to 1.0 x 10(7) cells mL(-1) through the decrease in photocurrent intensity resulting from its specific binding onto the photosensitive film, the detection limit being 5.0 x 10(3) cells mL(-1).

Authors+Show Affiliations

Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20181470

Citation

Qian, Zheng, et al. "A Photoelectrochemical Sensor Based On CdS-polyamidoamine Nano-composite Film for Cell Capture and Detection." Biosensors & Bioelectronics, vol. 25, no. 9, 2010, pp. 2045-50.
Qian Z, Bai HJ, Wang GL, et al. A photoelectrochemical sensor based on CdS-polyamidoamine nano-composite film for cell capture and detection. Biosens Bioelectron. 2010;25(9):2045-50.
Qian, Z., Bai, H. J., Wang, G. L., Xu, J. J., & Chen, H. Y. (2010). A photoelectrochemical sensor based on CdS-polyamidoamine nano-composite film for cell capture and detection. Biosensors & Bioelectronics, 25(9), 2045-50. https://doi.org/10.1016/j.bios.2010.01.036
Qian Z, et al. A Photoelectrochemical Sensor Based On CdS-polyamidoamine Nano-composite Film for Cell Capture and Detection. Biosens Bioelectron. 2010 May 15;25(9):2045-50. PubMed PMID: 20181470.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A photoelectrochemical sensor based on CdS-polyamidoamine nano-composite film for cell capture and detection. AU - Qian,Zheng, AU - Bai,Hai-Jing, AU - Wang,Guang-Li, AU - Xu,Jing-Juan, AU - Chen,Hong-Yuan, Y1 - 2010/02/04/ PY - 2009/12/30/received PY - 2010/01/27/accepted PY - 2010/2/26/entrez PY - 2010/2/26/pubmed PY - 2010/7/16/medline SP - 2045 EP - 50 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 25 IS - 9 N2 - We demonstrated herein a newly developed photoelectrochemical cell-sensor for the determination of SMMC-7721 human hepatoma carcinoma cells (SMMC-7721 cells) by using a photosensitive CdS-polyamidoamine (G4) nano-composite film (CdS-PAMAM). The film was generated by electrodeposition method. The presence of PAMAM in the film eliminated the surface defects of CdS nanoparticles and therefore resulted in a greatly enhanced photocurrent and a reduced dark current. In the presence of the electron donor ascorbic acid (AA), the photoexcitation of this modified electrode potentiostated at 0 V versus Ag/AgCl led to an anodic photocurrent. As a result of the covalent coupling reactions, a layer of concanavalin A (ConA) was firmly bound to the functionalized CdS-PAMAM film via glutaraldehyde bridges. The resulting modified electrodes were tested as sensors for SMMC-7721 cell capture and detection via affinity interactions between ConA and mannosyl groups on cell surface. The cell concentration was measured from 5.0 x 10(3) to 1.0 x 10(7) cells mL(-1) through the decrease in photocurrent intensity resulting from its specific binding onto the photosensitive film, the detection limit being 5.0 x 10(3) cells mL(-1). SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/20181470/A_photoelectrochemical_sensor_based_on_CdS_polyamidoamine_nano_composite_film_for_cell_capture_and_detection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(10)00066-7 DB - PRIME DP - Unbound Medicine ER -