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Bumetanide-induced enlargement of the rat intrastrial space and effects of a vasopressin type 2 antagonist.
ORL J Otorhinolaryngol Relat Spec. 2010; 71 Suppl 1:10-5.OJ

Abstract

OBJECTIVE

Water homeostasis is essential for the inner ear to maintain the function of hearing and equilibrium. Bumetanide inhibits Na(+)-K(+)-2Cl(-) co-transporter (NKCC), which is expressed in the basolateral membrane of stria vascularis marginal cell, and it causes the enlargement of intrastrial space. Aquaporin (AQP) 2 is expressed in the perilymph side of stria vascularis basal cell, and the vasopressin type 2 antagonist OPC-31260 produces downregulation of AQP2 mRNA levels in the inner ear. The aim of this study is to investigate the influence of OPC-31260 on experimentally induced enlargement of the intrastrial space caused by bumetanide.

MATERIALS AND METHODS

Wistar rats were divided into two groups, a BUM group and an OPC-BUM group. The BUM group was exposed to bumetanide, and the OPC-BUM group was exposed to bumetanide after being premedicated with OPC-31260. The specimens of the stria vascularis were observed using transmission electron microscopy and analyzed quantitatively and statistically.

RESULTS

Morphological changes of intrastrial space enlargement occurred in both the BUM and OPC-BUM groups. The ratio of the areas of the intrastrial space area to the stria vascularis were calculated, and the OPC-BUM group mean showed a minimal difference from the BUM-group mean. However, there is no statistical difference.

CONCLUSIONS

Premedication of rats with OPC-31260 tended to reduce bumetanide-induced enlargement of the intrastrial space. This may indicate that the effect of bumetanide on the stria vascularis is much stronger than that of OPC-31260.

Authors+Show Affiliations

Department of Otolaryngology, Osaka University Graduate School of Medicine, Osaka, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20185944

Citation

Nishimura, Masahiko, et al. "Bumetanide-induced Enlargement of the Rat Intrastrial Space and Effects of a Vasopressin Type 2 Antagonist." ORL; Journal for Oto-rhino-laryngology and Its Related Specialties, vol. 71 Suppl 1, 2010, pp. 10-5.
Nishimura M, Kakigi A, Takeda T, et al. Bumetanide-induced enlargement of the rat intrastrial space and effects of a vasopressin type 2 antagonist. ORL J Otorhinolaryngol Relat Spec. 2010;71 Suppl 1:10-5.
Nishimura, M., Kakigi, A., Takeda, T., Okada, T., & Doi, K. (2010). Bumetanide-induced enlargement of the rat intrastrial space and effects of a vasopressin type 2 antagonist. ORL; Journal for Oto-rhino-laryngology and Its Related Specialties, 71 Suppl 1, 10-5. https://doi.org/10.1159/000265114
Nishimura M, et al. Bumetanide-induced Enlargement of the Rat Intrastrial Space and Effects of a Vasopressin Type 2 Antagonist. ORL J Otorhinolaryngol Relat Spec. 2010;71 Suppl 1:10-5. PubMed PMID: 20185944.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bumetanide-induced enlargement of the rat intrastrial space and effects of a vasopressin type 2 antagonist. AU - Nishimura,Masahiko, AU - Kakigi,Akinobu, AU - Takeda,Taizo, AU - Okada,Teruhiko, AU - Doi,Katsumi, Y1 - 2010/02/24/ PY - 2010/2/27/entrez PY - 2010/3/10/pubmed PY - 2010/6/11/medline SP - 10 EP - 5 JF - ORL; journal for oto-rhino-laryngology and its related specialties JO - ORL J Otorhinolaryngol Relat Spec VL - 71 Suppl 1 N2 - OBJECTIVE: Water homeostasis is essential for the inner ear to maintain the function of hearing and equilibrium. Bumetanide inhibits Na(+)-K(+)-2Cl(-) co-transporter (NKCC), which is expressed in the basolateral membrane of stria vascularis marginal cell, and it causes the enlargement of intrastrial space. Aquaporin (AQP) 2 is expressed in the perilymph side of stria vascularis basal cell, and the vasopressin type 2 antagonist OPC-31260 produces downregulation of AQP2 mRNA levels in the inner ear. The aim of this study is to investigate the influence of OPC-31260 on experimentally induced enlargement of the intrastrial space caused by bumetanide. MATERIALS AND METHODS: Wistar rats were divided into two groups, a BUM group and an OPC-BUM group. The BUM group was exposed to bumetanide, and the OPC-BUM group was exposed to bumetanide after being premedicated with OPC-31260. The specimens of the stria vascularis were observed using transmission electron microscopy and analyzed quantitatively and statistically. RESULTS: Morphological changes of intrastrial space enlargement occurred in both the BUM and OPC-BUM groups. The ratio of the areas of the intrastrial space area to the stria vascularis were calculated, and the OPC-BUM group mean showed a minimal difference from the BUM-group mean. However, there is no statistical difference. CONCLUSIONS: Premedication of rats with OPC-31260 tended to reduce bumetanide-induced enlargement of the intrastrial space. This may indicate that the effect of bumetanide on the stria vascularis is much stronger than that of OPC-31260. SN - 1423-0275 UR - https://www.unboundmedicine.com/medline/citation/20185944/Bumetanide_induced_enlargement_of_the_rat_intrastrial_space_and_effects_of_a_vasopressin_type_2_antagonist_ L2 - https://www.karger.com?DOI=10.1159/000265114 DB - PRIME DP - Unbound Medicine ER -