Abstract
OBJECTIVE
To investigate the relationship between endolymphatic hydrops and perilymphatic potassium.
METHODS
20 pigmented guinea pigs were used: 10 for scala vestibuli study and 10 for scala tympani study. Acute endolymphatic hydrops was produced by microinjection of an artificial endolymph into the scala media. Injections were performed in the second turn at rates up to 500 nl/min for a period of 10 min. The injection volume was up to 5 microl. Endocochlear potential (EP) was monitored during injections. Simultaneous with the injections, the potassium concentrations in scala vestibuli (K(SV)) or tympani (K(ST)) perilymph were measured with ion-sensitive double-barreled microelectrodes sealed into in the scalae in the 3rd turn with cyanoacrylate glue.
RESULTS
For endolymphatic injections of <or=3 microl, perilymphatic K(SV) and K(ST) changes were generally small. With larger volume injections, substantial increases in both K(ST) and K(SV) were observed, with larger increases observed in K(SV) compared to K(ST).
CONCLUSION
An increase of perilymph potassium ion concentration is associated with endolymphatic hydrops and the perilymphatic increase could contribute to the inner ear dysfunction of patients with Ménière's disease. Although mild hydrops may not cause perilymphatic changes, extended hydrops may increase perilymphatic K which could contribute to vertigo and/or hearing loss during the attack.
TY - JOUR
T1 - Effect of artificial endolymph injection into the cochlear duct on perilymph potassium.
AU - Kakigi,Akinobu,
AU - Salt,Alec N,
AU - Takeda,Taizo,
Y1 - 2010/02/24/
PY - 2010/2/27/entrez
PY - 2010/3/10/pubmed
PY - 2010/6/11/medline
SP - 16
EP - 8
JF - ORL; journal for oto-rhino-laryngology and its related specialties
JO - ORL J. Otorhinolaryngol. Relat. Spec.
VL - 71 Suppl 1
N2 - OBJECTIVE: To investigate the relationship between endolymphatic hydrops and perilymphatic potassium. METHODS: 20 pigmented guinea pigs were used: 10 for scala vestibuli study and 10 for scala tympani study. Acute endolymphatic hydrops was produced by microinjection of an artificial endolymph into the scala media. Injections were performed in the second turn at rates up to 500 nl/min for a period of 10 min. The injection volume was up to 5 microl. Endocochlear potential (EP) was monitored during injections. Simultaneous with the injections, the potassium concentrations in scala vestibuli (K(SV)) or tympani (K(ST)) perilymph were measured with ion-sensitive double-barreled microelectrodes sealed into in the scalae in the 3rd turn with cyanoacrylate glue. RESULTS: For endolymphatic injections of <or=3 microl, perilymphatic K(SV) and K(ST) changes were generally small. With larger volume injections, substantial increases in both K(ST) and K(SV) were observed, with larger increases observed in K(SV) compared to K(ST). CONCLUSION: An increase of perilymph potassium ion concentration is associated with endolymphatic hydrops and the perilymphatic increase could contribute to the inner ear dysfunction of patients with Ménière's disease. Although mild hydrops may not cause perilymphatic changes, extended hydrops may increase perilymphatic K which could contribute to vertigo and/or hearing loss during the attack.
SN - 1423-0275
UR - https://www.unboundmedicine.com/medline/citation/20185945/Effect_of_artificial_endolymph_injection_into_the_cochlear_duct_on_perilymph_potassium_
L2 - https://www.karger.com?DOI=10.1159/000265118
DB - PRIME
DP - Unbound Medicine
ER -
