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Analysis and tentative structure elucidation of new anthocyanins in fruit peel of Vitis coignetiae Pulliat (meoru) using LC-MS/MS: Contribution to the overall antioxidant activity.
J Sep Sci. 2010 May; 33(9):1192-7.JS

Abstract

The skin of Vitis coignetiae Pulliat (meoru) grown wild in the Republic of Korea was analyzed for anthocyanins via HPLC coupled to ESI-MS/MS in positive ion mode. Chromatographic separation was conducted via RP HPLC using a C(18) column, with a 50-min gradient from 0 to 80% methanol in water containing 0.5% formic acid. A total of 18 anthocyanins were identified. Among them, nine compounds were newly determined by comparing the retention time (t(R)) and mass fragmentation patterns with those of the previously reported anthocyanins for other grape varieties: malvidin hexose, peonidin 3-galactoside, malvidin 3-galactoside, cyanidin, petunidin, petunidin 3-(6''-coumaroyl)-5-diglucoside, peonidin, malvidin, and malvidin 3-(6''-coumaroyl)-5-diglucoside. The antioxidant activity of the V. coignetiae Pulliat anthocyanins was determined via 2,2-diphenyl-2-picrylhydrazyl radical scavenging and 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) radical cation assays in a range of concentration from 25 to 500 mg/L. The capacity increased with concentration. The IC(50) values, defined as the concentration of sample required to scavenge 50% of free radicals, were calculated as follows: 189.63+/-11.31 mg/L and 141.29+/-6.70 mg/L for 2,2-diphenyl-2-picrylhydrazyl and 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) radical cation, respectively. The antioxidant activity of the V. coignetiae Pulliat anthocyanins is substantially higher than that of ascorbic acid and is similar to the effects of the extracts obtained from other grape varieties.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Choi JY
Department of Chemistry and Research Institute of Life Science, Gyeongsang National University, Jinju, Republic of Korea.
Lee SJ
No affiliation info available
Lee SJ
No affiliation info available
Park S
No affiliation info available
Lee JH
No affiliation info available
Shim JH
No affiliation info available
Abd El-Aty AM
No affiliation info available
Jin JS
No affiliation info available
Jeong ED
No affiliation info available
Lee WS
No affiliation info available
Shin SC
No affiliation info available

MeSH

AnthocyaninsAntioxidantsChromatography, High Pressure LiquidMolecular StructureTandem Mass SpectrometryVitis

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20187032

Citation

Choi, Jin Young, et al. "Analysis and Tentative Structure Elucidation of New Anthocyanins in Fruit Peel of Vitis Coignetiae Pulliat (meoru) Using LC-MS/MS: Contribution to the Overall Antioxidant Activity." Journal of Separation Science, vol. 33, no. 9, 2010, pp. 1192-7.
Choi JY, Lee SJ, Lee SJ, et al. Analysis and tentative structure elucidation of new anthocyanins in fruit peel of Vitis coignetiae Pulliat (meoru) using LC-MS/MS: Contribution to the overall antioxidant activity. J Sep Sci. 2010;33(9):1192-7.
Choi, J. Y., Lee, S. J., Lee, S. J., Park, S., Lee, J. H., Shim, J. H., Abd El-Aty, A. M., Jin, J. S., Jeong, E. D., Lee, W. S., & Shin, S. C. (2010). Analysis and tentative structure elucidation of new anthocyanins in fruit peel of Vitis coignetiae Pulliat (meoru) using LC-MS/MS: Contribution to the overall antioxidant activity. Journal of Separation Science, 33(9), 1192-7. https://doi.org/10.1002/jssc.200900748
Choi JY, et al. Analysis and Tentative Structure Elucidation of New Anthocyanins in Fruit Peel of Vitis Coignetiae Pulliat (meoru) Using LC-MS/MS: Contribution to the Overall Antioxidant Activity. J Sep Sci. 2010;33(9):1192-7. PubMed PMID: 20187032.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis and tentative structure elucidation of new anthocyanins in fruit peel of Vitis coignetiae Pulliat (meoru) using LC-MS/MS: Contribution to the overall antioxidant activity. AU - Choi,Jin Young, AU - Lee,Soo Jung, AU - Lee,Sung Joong, AU - Park,Semin, AU - Lee,Jung Han, AU - Shim,Jae-Han, AU - Abd El-Aty,A M, AU - Jin,Jong-Sung, AU - Jeong,Euh-Duck, AU - Lee,Won Sup, AU - Shin,Sung Chul, PY - 2010/2/27/entrez PY - 2010/2/27/pubmed PY - 2010/7/30/medline SP - 1192 EP - 7 JF - Journal of separation science JO - J Sep Sci VL - 33 IS - 9 N2 - The skin of Vitis coignetiae Pulliat (meoru) grown wild in the Republic of Korea was analyzed for anthocyanins via HPLC coupled to ESI-MS/MS in positive ion mode. Chromatographic separation was conducted via RP HPLC using a C(18) column, with a 50-min gradient from 0 to 80% methanol in water containing 0.5% formic acid. A total of 18 anthocyanins were identified. Among them, nine compounds were newly determined by comparing the retention time (t(R)) and mass fragmentation patterns with those of the previously reported anthocyanins for other grape varieties: malvidin hexose, peonidin 3-galactoside, malvidin 3-galactoside, cyanidin, petunidin, petunidin 3-(6''-coumaroyl)-5-diglucoside, peonidin, malvidin, and malvidin 3-(6''-coumaroyl)-5-diglucoside. The antioxidant activity of the V. coignetiae Pulliat anthocyanins was determined via 2,2-diphenyl-2-picrylhydrazyl radical scavenging and 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) radical cation assays in a range of concentration from 25 to 500 mg/L. The capacity increased with concentration. The IC(50) values, defined as the concentration of sample required to scavenge 50% of free radicals, were calculated as follows: 189.63+/-11.31 mg/L and 141.29+/-6.70 mg/L for 2,2-diphenyl-2-picrylhydrazyl and 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) radical cation, respectively. The antioxidant activity of the V. coignetiae Pulliat anthocyanins is substantially higher than that of ascorbic acid and is similar to the effects of the extracts obtained from other grape varieties. SN - 1615-9314 UR - https://www.unboundmedicine.com/medline/citation/20187032/Analysis_and_tentative_structure_elucidation_of_new_anthocyanins_in_fruit_peel_of_Vitis_coignetiae_Pulliat__meoru__using_LC_MS/MS:_Contribution_to_the_overall_antioxidant_activity_ L2 - https://doi.org/10.1002/jssc.200900748 DB - PRIME DP - Unbound Medicine ER -