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Insulin-like growth factor-1 modulates Ca2+ homeostasis and apoptosis of cultured dorsal root ganglion neurons with excitotoxicity induced by glutamate.
Pharmazie. 2010 Jan; 65(1):5-8.P

Abstract

Insulin-like growth factor-1 (IGF-1) is a neurotrophic factor and a potent anti-apoptotic factor. IGF-1 plays an important role in promoting axonal growth from dorsal root ganglion (DRG) neurons and prevents apoptosis in DRG neurons. Whether IGF-1 could modulate Ca2+ homeostasis and apoptosis of sensory DRG neurons with excitotoxicity induced by glutamate (Glu) is still unknown. In the present study, primary cultured DRG neurons were used to determine the effects of IGF-1 on Ca2+ homeostasis and apoptosis of sensory DRG neurons with excitotoxicity induced by Glu. Intracellular Ca2+ concentration ([Ca2+]i) in isolated DRG neurons using the fluorescent Ca2+ indicator fura-3 was measured by confocal laser scanning microscope (CLSM). Procaspase-3 expression was detected by Western blot analysis. Application of 0.2 mmol/L Glu evoked an increase in [Ca2+]i, confirming the excitatory effect of Glu at this stage. The decrease of procaspase-3 expression levels after application of 0.2 mmol/L Glu suggested the apoptotic effects of Glu. These effects could be inhibited by the presence of IGF-1. In conclusion, we demonstrated that IGF-1 could modulate Ca2+ homeostasis and apoptosis of sensory DRG neurons with excitotoxicity induced by Glu. Both Ca2+ homeostasis and caspase-3 processing were implicated as the underlying neuroprotective mechanisms of IGF-1.

Authors+Show Affiliations

Department of Anatomy, Shandong University School of Medicine, Jinan, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20187571

Citation

Zhang, Fengjun, et al. "Insulin-like Growth Factor-1 Modulates Ca2+ Homeostasis and Apoptosis of Cultured Dorsal Root Ganglion Neurons With Excitotoxicity Induced By Glutamate." Die Pharmazie, vol. 65, no. 1, 2010, pp. 5-8.
Zhang F, Wang L, Liu Z, et al. Insulin-like growth factor-1 modulates Ca2+ homeostasis and apoptosis of cultured dorsal root ganglion neurons with excitotoxicity induced by glutamate. Pharmazie. 2010;65(1):5-8.
Zhang, F., Wang, L., Liu, Z., Xing, Z., Wang, H., & Li, Z. (2010). Insulin-like growth factor-1 modulates Ca2+ homeostasis and apoptosis of cultured dorsal root ganglion neurons with excitotoxicity induced by glutamate. Die Pharmazie, 65(1), 5-8.
Zhang F, et al. Insulin-like Growth Factor-1 Modulates Ca2+ Homeostasis and Apoptosis of Cultured Dorsal Root Ganglion Neurons With Excitotoxicity Induced By Glutamate. Pharmazie. 2010;65(1):5-8. PubMed PMID: 20187571.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Insulin-like growth factor-1 modulates Ca2+ homeostasis and apoptosis of cultured dorsal root ganglion neurons with excitotoxicity induced by glutamate. AU - Zhang,Fengjun, AU - Wang,Lihong, AU - Liu,Zhen, AU - Xing,Ziying, AU - Wang,Huaijing, AU - Li,Zhenzhong, PY - 2010/3/2/entrez PY - 2010/3/2/pubmed PY - 2010/3/26/medline SP - 5 EP - 8 JF - Die Pharmazie JO - Pharmazie VL - 65 IS - 1 N2 - Insulin-like growth factor-1 (IGF-1) is a neurotrophic factor and a potent anti-apoptotic factor. IGF-1 plays an important role in promoting axonal growth from dorsal root ganglion (DRG) neurons and prevents apoptosis in DRG neurons. Whether IGF-1 could modulate Ca2+ homeostasis and apoptosis of sensory DRG neurons with excitotoxicity induced by glutamate (Glu) is still unknown. In the present study, primary cultured DRG neurons were used to determine the effects of IGF-1 on Ca2+ homeostasis and apoptosis of sensory DRG neurons with excitotoxicity induced by Glu. Intracellular Ca2+ concentration ([Ca2+]i) in isolated DRG neurons using the fluorescent Ca2+ indicator fura-3 was measured by confocal laser scanning microscope (CLSM). Procaspase-3 expression was detected by Western blot analysis. Application of 0.2 mmol/L Glu evoked an increase in [Ca2+]i, confirming the excitatory effect of Glu at this stage. The decrease of procaspase-3 expression levels after application of 0.2 mmol/L Glu suggested the apoptotic effects of Glu. These effects could be inhibited by the presence of IGF-1. In conclusion, we demonstrated that IGF-1 could modulate Ca2+ homeostasis and apoptosis of sensory DRG neurons with excitotoxicity induced by Glu. Both Ca2+ homeostasis and caspase-3 processing were implicated as the underlying neuroprotective mechanisms of IGF-1. SN - 0031-7144 UR - https://www.unboundmedicine.com/medline/citation/20187571/Insulin_like_growth_factor_1_modulates_Ca2+_homeostasis_and_apoptosis_of_cultured_dorsal_root_ganglion_neurons_with_excitotoxicity_induced_by_glutamate_ DB - PRIME DP - Unbound Medicine ER -