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Surface-enhanced Raman scattering-based label-free microarray readout for the detection of microorganisms.
Anal Chem. 2010 Apr 01; 82(7):2766-72.AC

Abstract

We report the development of a new technique for label-free microarray readout based on surface-enhanced Raman scattering (SERS). In doing so, an investigation on optimized SERS substrates for the application to in situ microorganism analysis by Raman microscopy was carried out. Chemically synthesized nanoparticles were successfully applied to an immunoassay for label-free detection of single microorganisms. In this way, species specific, reproducible, and strong SERS spectra were collected from different bacteria immobilized on a chip. Furthermore, quantitative analysis of the microorganisms was performed using Raman mapping. Unlike conventional SERS detection of bacteria, which requires dehydration prior to analysis, our system enables us to detect and quantify microorganisms in an aqueous environment in situ. Hence, the nondestructive analysis of living bacteria cells is possible. Moreover, the "whole-organism fingerprint" SERS spectra can be adopted for further chemical characterization of microorganisms.

Authors+Show Affiliations

Institute of Hydrochemistry and Chair for Analytical Chemistry, Technische Universität München, Marchioninistrasse 17, D-81377 Munich, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20196561

Citation

Knauer, Maria, et al. "Surface-enhanced Raman Scattering-based Label-free Microarray Readout for the Detection of Microorganisms." Analytical Chemistry, vol. 82, no. 7, 2010, pp. 2766-72.
Knauer M, Ivleva NP, Liu X, et al. Surface-enhanced Raman scattering-based label-free microarray readout for the detection of microorganisms. Anal Chem. 2010;82(7):2766-72.
Knauer, M., Ivleva, N. P., Liu, X., Niessner, R., & Haisch, C. (2010). Surface-enhanced Raman scattering-based label-free microarray readout for the detection of microorganisms. Analytical Chemistry, 82(7), 2766-72. https://doi.org/10.1021/ac902696y
Knauer M, et al. Surface-enhanced Raman Scattering-based Label-free Microarray Readout for the Detection of Microorganisms. Anal Chem. 2010 Apr 1;82(7):2766-72. PubMed PMID: 20196561.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Surface-enhanced Raman scattering-based label-free microarray readout for the detection of microorganisms. AU - Knauer,Maria, AU - Ivleva,Natalia P, AU - Liu,Xiangjiang, AU - Niessner,Reinhard, AU - Haisch,Christoph, PY - 2010/3/4/entrez PY - 2010/3/4/pubmed PY - 2010/6/24/medline SP - 2766 EP - 72 JF - Analytical chemistry JO - Anal Chem VL - 82 IS - 7 N2 - We report the development of a new technique for label-free microarray readout based on surface-enhanced Raman scattering (SERS). In doing so, an investigation on optimized SERS substrates for the application to in situ microorganism analysis by Raman microscopy was carried out. Chemically synthesized nanoparticles were successfully applied to an immunoassay for label-free detection of single microorganisms. In this way, species specific, reproducible, and strong SERS spectra were collected from different bacteria immobilized on a chip. Furthermore, quantitative analysis of the microorganisms was performed using Raman mapping. Unlike conventional SERS detection of bacteria, which requires dehydration prior to analysis, our system enables us to detect and quantify microorganisms in an aqueous environment in situ. Hence, the nondestructive analysis of living bacteria cells is possible. Moreover, the "whole-organism fingerprint" SERS spectra can be adopted for further chemical characterization of microorganisms. SN - 1520-6882 UR - https://www.unboundmedicine.com/medline/citation/20196561/Surface_enhanced_Raman_scattering_based_label_free_microarray_readout_for_the_detection_of_microorganisms_ L2 - https://doi.org/10.1021/ac902696y DB - PRIME DP - Unbound Medicine ER -