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Characterization of Lewy body pathology in 12- and 16-year-old intrastriatal mesencephalic grafts surviving in a patient with Parkinson's disease.
Mov Disord. 2010 Jun 15; 25(8):1091-6.MD

Abstract

We previously reported the occurrence of Lewy bodies in grafted human fetal mesencephalic neurons in two patients with Parkinson's disease. Here, we have used immunohistochemistry and electron microscopy to characterize the development of Lewy bodies in one of these cases. This patient was operated in putamen on both sides at 12 or 16 years before death, respectively. We demonstrate that 2% of the 12-year-old and 5% of the 16-year-old grafted, presumed dopaminergic neurons contained Lewy bodies immunoreactive for alpha-synuclein. Based on morphological analysis, two forms of alpha-synuclein-positive aggregates were distinguished in the grafts, the first a classical and compact Lewy body, the other a loose meshwork aggregate. Lewy bodies in the grafts stained positively for ubiquitin and thioflavin-S, and contained characteristic alpha-synuclein immunoreactive electron dense fibrillar structures on electron microscopy. Our data indicate that Lewy bodies develop gradually in transplanted dopaminergic neurons in a fashion similar to that in dopaminergic neurons in the host substantia nigra.

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Authors+Show Affiliations

Li JY
Neuronal Survival Unit, Wallenberg Neuroscience Center, Lund University, Lund, Sweden. jia-yi.li@med.lu.se
Englund E
No affiliation info available
Widner H
No affiliation info available
Rehncrona S
No affiliation info available
Björklund A
No affiliation info available
Lindvall O
No affiliation info available
Brundin P
No affiliation info available

MeSH

BenzothiazolesFetal Tissue TransplantationHumansLewy BodiesLongitudinal StudiesMaleMesencephalonMicroscopy, Electron, TransmissionMiddle AgedNeuronsParkinson DiseasePostmortem ChangesThiazolesTime Factorsalpha-Synuclein

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20198645

Citation

Li, Jia-Yi, et al. "Characterization of Lewy Body Pathology in 12- and 16-year-old Intrastriatal Mesencephalic Grafts Surviving in a Patient With Parkinson's Disease." Movement Disorders : Official Journal of the Movement Disorder Society, vol. 25, no. 8, 2010, pp. 1091-6.
Li JY, Englund E, Widner H, et al. Characterization of Lewy body pathology in 12- and 16-year-old intrastriatal mesencephalic grafts surviving in a patient with Parkinson's disease. Mov Disord. 2010;25(8):1091-6.
Li, J. Y., Englund, E., Widner, H., Rehncrona, S., Björklund, A., Lindvall, O., & Brundin, P. (2010). Characterization of Lewy body pathology in 12- and 16-year-old intrastriatal mesencephalic grafts surviving in a patient with Parkinson's disease. Movement Disorders : Official Journal of the Movement Disorder Society, 25(8), 1091-6. https://doi.org/10.1002/mds.23012
Li JY, et al. Characterization of Lewy Body Pathology in 12- and 16-year-old Intrastriatal Mesencephalic Grafts Surviving in a Patient With Parkinson's Disease. Mov Disord. 2010 Jun 15;25(8):1091-6. PubMed PMID: 20198645.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of Lewy body pathology in 12- and 16-year-old intrastriatal mesencephalic grafts surviving in a patient with Parkinson's disease. AU - Li,Jia-Yi, AU - Englund,Elisabet, AU - Widner,Håkan, AU - Rehncrona,Stig, AU - Björklund,Anders, AU - Lindvall,Olle, AU - Brundin,Patrik, PY - 2010/3/4/entrez PY - 2010/3/4/pubmed PY - 2010/9/21/medline SP - 1091 EP - 6 JF - Movement disorders : official journal of the Movement Disorder Society JO - Mov Disord VL - 25 IS - 8 N2 - We previously reported the occurrence of Lewy bodies in grafted human fetal mesencephalic neurons in two patients with Parkinson's disease. Here, we have used immunohistochemistry and electron microscopy to characterize the development of Lewy bodies in one of these cases. This patient was operated in putamen on both sides at 12 or 16 years before death, respectively. We demonstrate that 2% of the 12-year-old and 5% of the 16-year-old grafted, presumed dopaminergic neurons contained Lewy bodies immunoreactive for alpha-synuclein. Based on morphological analysis, two forms of alpha-synuclein-positive aggregates were distinguished in the grafts, the first a classical and compact Lewy body, the other a loose meshwork aggregate. Lewy bodies in the grafts stained positively for ubiquitin and thioflavin-S, and contained characteristic alpha-synuclein immunoreactive electron dense fibrillar structures on electron microscopy. Our data indicate that Lewy bodies develop gradually in transplanted dopaminergic neurons in a fashion similar to that in dopaminergic neurons in the host substantia nigra. SN - 1531-8257 UR - https://www.unboundmedicine.com/medline/citation/20198645/Characterization_of_Lewy_body_pathology_in_12__and_16_year_old_intrastriatal_mesencephalic_grafts_surviving_in_a_patient_with_Parkinson's_disease_ L2 - https://doi.org/10.1002/mds.23012 DB - PRIME DP - Unbound Medicine ER -