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Effects of moniliformin and selenium on human articular cartilage metabolism and their potential relationships to the pathogenesis of Kashin-Beck disease.
J Zhejiang Univ Sci B. 2010 Mar; 11(3):200-8.JZ

Abstract

OBJECTIVE

To investigate the effects of mycotoxin moniliformin (MON) on the metabolism of aggrecan and type II collagen in human chondrocytes in vitro and the relationship between MON and Kashin-Beck disease (KBD).

METHODS

Human chondrocytes were isolated and cultured on bone matrix gelatin to form an artificial cartilage model in vitro with or without MON toxin. Cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The expression of aggrecan and type II collagen in the cartilage was determined using immunocytochemical staining.

RESULTS

MON toxin inhibited chondrocyte viability in dose-dependent and time-dependent manners. MON reduced aggrecan and type II collagen syntheses in the tissue-engineered cartilage. MON also increased the expression of matrix metalloproteinase-1 (MMP-1), MMP-13, BC4 epitopes, and CD44 in cartilages. However, the expression of 3B3(-) epitopes in cartilages was inhibited by MON. Selenium partially alleviated the damage of aggrecan induced by MON toxin.

CONCLUSION

MON toxin promoted the catabolism of aggrecan and type II collagen in human chondrocytes.

Authors+Show Affiliations

Institute of Endemic Diseases, School of Medicine, Xi'an Jiaotong University, Xi'an 710061, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20205306

Citation

Zhang, An, et al. "Effects of Moniliformin and Selenium On Human Articular Cartilage Metabolism and Their Potential Relationships to the Pathogenesis of Kashin-Beck Disease." Journal of Zhejiang University. Science. B, vol. 11, no. 3, 2010, pp. 200-8.
Zhang A, Cao JL, Yang B, et al. Effects of moniliformin and selenium on human articular cartilage metabolism and their potential relationships to the pathogenesis of Kashin-Beck disease. J Zhejiang Univ Sci B. 2010;11(3):200-8.
Zhang, A., Cao, J. L., Yang, B., Chen, J. H., Zhang, Z. T., Li, S. Y., Fu, Q., Hugnes, C. E., & Caterson, B. (2010). Effects of moniliformin and selenium on human articular cartilage metabolism and their potential relationships to the pathogenesis of Kashin-Beck disease. Journal of Zhejiang University. Science. B, 11(3), 200-8. https://doi.org/10.1631/jzus.B0900074
Zhang A, et al. Effects of Moniliformin and Selenium On Human Articular Cartilage Metabolism and Their Potential Relationships to the Pathogenesis of Kashin-Beck Disease. J Zhejiang Univ Sci B. 2010;11(3):200-8. PubMed PMID: 20205306.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of moniliformin and selenium on human articular cartilage metabolism and their potential relationships to the pathogenesis of Kashin-Beck disease. AU - Zhang,An, AU - Cao,Jun-ling, AU - Yang,Bo, AU - Chen,Jing-hong, AU - Zhang,Zeng-tie, AU - Li,Si-yuan, AU - Fu,Qiang, AU - Hugnes,Clare E, AU - Caterson,Bruce, PY - 2010/3/6/entrez PY - 2010/3/6/pubmed PY - 2010/6/11/medline SP - 200 EP - 8 JF - Journal of Zhejiang University. Science. B JO - J Zhejiang Univ Sci B VL - 11 IS - 3 N2 - OBJECTIVE: To investigate the effects of mycotoxin moniliformin (MON) on the metabolism of aggrecan and type II collagen in human chondrocytes in vitro and the relationship between MON and Kashin-Beck disease (KBD). METHODS: Human chondrocytes were isolated and cultured on bone matrix gelatin to form an artificial cartilage model in vitro with or without MON toxin. Cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The expression of aggrecan and type II collagen in the cartilage was determined using immunocytochemical staining. RESULTS: MON toxin inhibited chondrocyte viability in dose-dependent and time-dependent manners. MON reduced aggrecan and type II collagen syntheses in the tissue-engineered cartilage. MON also increased the expression of matrix metalloproteinase-1 (MMP-1), MMP-13, BC4 epitopes, and CD44 in cartilages. However, the expression of 3B3(-) epitopes in cartilages was inhibited by MON. Selenium partially alleviated the damage of aggrecan induced by MON toxin. CONCLUSION: MON toxin promoted the catabolism of aggrecan and type II collagen in human chondrocytes. SN - 1862-1783 UR - https://www.unboundmedicine.com/medline/citation/20205306/Effects_of_moniliformin_and_selenium_on_human_articular_cartilage_metabolism_and_their_potential_relationships_to_the_pathogenesis_of_Kashin_Beck_disease_ L2 - http://www.jzus.zju.edu.cn/article.php?doi=10.1631/jzus.B0900074 DB - PRIME DP - Unbound Medicine ER -