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Characterization of IgE epitopes of Cuc m 2, the major melon allergen, and their role in cross-reactivity with pollen profilins.
Clin Exp Allergy. 2010 Jan; 40(1):174-81.CE

Abstract

BACKGROUND

Plant profilins are described as minor allergens, although with some exceptions in foods such as melon, watermelon or orange. In fact, they could be responsible for many cross-reactions among distantly related species. This is likely to be a consequence of the presence of common epitopes.

OBJECTIVE

To characterize the B epitopes of Cuc m 2, a model of plant food profilin, using phage display techniques and to compare with other profilins, such as those of timothy grass and birch pollen, and human I profilin, to understand the mechanism of cross-reaction among members of this family.

METHODS

IgE of melon-allergic patients was used to select clones from a phage display 12 mer peptide library. After two rounds of screening, Cuc m 2-specific clones were eluted and the DNA insertion sequenced. The residues of each clone were mapped on the Cuc m 2 surface to define a mimotope, which was also localized on the three-dimensional surfaces of other profilins.

RESULTS

Seventeen melon-allergic patients were selected. Sera from each of them recognized the melon profilin, Cuc m 2, but the majority also recognized Phl p 12 or Bet v 2, timothy grass-, and birch-pollen profilins, respectively. A Cuc m 2 mimotope was defined and mapped onto its surface giving the following sequence: S(2)W(3)A(5)Y(6)D(9)H(10)T(111)P(112)G(113)Q(114)N(116)M(117)R(121)L(122). The homologous residues in Phl p 12 and Bet v 2 had almost identical sequences. By contrast, the homologous sequence in human profilin showed many differences.

CONCLUSIONS

The identified mimotope could be involved in cross-reactions among food and pollen profilins. Many of these cross-reactions observed in the clinical realm could be explained by the presence of a common epitope found in food and pollen allergens. A new strategy of immunotherapy based on this IgE region could be used in alternative immunotherapy strategies.

Authors+Show Affiliations

Departamento de Biotecnología, Unidad de Bioquímica, E.T.S. Ingenieros Agrónomos, UPM, Madrid, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20205701

Citation

Tordesillas, L, et al. "Characterization of IgE Epitopes of Cuc M 2, the Major Melon Allergen, and Their Role in Cross-reactivity With Pollen Profilins." Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology, vol. 40, no. 1, 2010, pp. 174-81.
Tordesillas L, Pacios LF, Palacín A, et al. Characterization of IgE epitopes of Cuc m 2, the major melon allergen, and their role in cross-reactivity with pollen profilins. Clin Exp Allergy. 2010;40(1):174-81.
Tordesillas, L., Pacios, L. F., Palacín, A., Cuesta-Herranz, J., Madero, M., & Díaz-Perales, A. (2010). Characterization of IgE epitopes of Cuc m 2, the major melon allergen, and their role in cross-reactivity with pollen profilins. Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology, 40(1), 174-81. https://doi.org/10.1111/j.1365-2222.2009.03401.x
Tordesillas L, et al. Characterization of IgE Epitopes of Cuc M 2, the Major Melon Allergen, and Their Role in Cross-reactivity With Pollen Profilins. Clin Exp Allergy. 2010;40(1):174-81. PubMed PMID: 20205701.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of IgE epitopes of Cuc m 2, the major melon allergen, and their role in cross-reactivity with pollen profilins. AU - Tordesillas,L, AU - Pacios,L F, AU - Palacín,A, AU - Cuesta-Herranz,J, AU - Madero,M, AU - Díaz-Perales,A, PY - 2010/3/9/entrez PY - 2010/3/9/pubmed PY - 2010/6/19/medline SP - 174 EP - 81 JF - Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology JO - Clin Exp Allergy VL - 40 IS - 1 N2 - BACKGROUND: Plant profilins are described as minor allergens, although with some exceptions in foods such as melon, watermelon or orange. In fact, they could be responsible for many cross-reactions among distantly related species. This is likely to be a consequence of the presence of common epitopes. OBJECTIVE: To characterize the B epitopes of Cuc m 2, a model of plant food profilin, using phage display techniques and to compare with other profilins, such as those of timothy grass and birch pollen, and human I profilin, to understand the mechanism of cross-reaction among members of this family. METHODS: IgE of melon-allergic patients was used to select clones from a phage display 12 mer peptide library. After two rounds of screening, Cuc m 2-specific clones were eluted and the DNA insertion sequenced. The residues of each clone were mapped on the Cuc m 2 surface to define a mimotope, which was also localized on the three-dimensional surfaces of other profilins. RESULTS: Seventeen melon-allergic patients were selected. Sera from each of them recognized the melon profilin, Cuc m 2, but the majority also recognized Phl p 12 or Bet v 2, timothy grass-, and birch-pollen profilins, respectively. A Cuc m 2 mimotope was defined and mapped onto its surface giving the following sequence: S(2)W(3)A(5)Y(6)D(9)H(10)T(111)P(112)G(113)Q(114)N(116)M(117)R(121)L(122). The homologous residues in Phl p 12 and Bet v 2 had almost identical sequences. By contrast, the homologous sequence in human profilin showed many differences. CONCLUSIONS: The identified mimotope could be involved in cross-reactions among food and pollen profilins. Many of these cross-reactions observed in the clinical realm could be explained by the presence of a common epitope found in food and pollen allergens. A new strategy of immunotherapy based on this IgE region could be used in alternative immunotherapy strategies. SN - 1365-2222 UR - https://www.unboundmedicine.com/medline/citation/20205701/Characterization_of_IgE_epitopes_of_Cuc_m_2_the_major_melon_allergen_and_their_role_in_cross_reactivity_with_pollen_profilins_ L2 - https://doi.org/10.1111/j.1365-2222.2009.03401.x DB - PRIME DP - Unbound Medicine ER -