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Use of hematological parameters to assess the efficiency of quince (Cydonia oblonga Miller) leaf extract in alleviation of the effect of ultraviolet--A radiation on African catfish Clarias gariepinus (Burchell, 1822).
J Photochem Photobiol B. 2010 Apr 02; 99(1):1-8.JP

Abstract

The present study aimed to elucidate the negative impacts of UVA on some biochemical and hematological variables of the economically important African catfish, Clarias gariepinus and investigates the putative role of quince (Cydonia oblonga Miller) leaf extract in protection and/or alleviation of such negative impacts. Changes in the hematological and blood biochemical values often reflect alteration of physiological state. Blood parameters can be useful for the measurement of physiological disturbances in stressed fish and thus provide information about the level of damage in the fish. We found a significant (P<0.05) decrease in the red blood cell counts, hemoglobin and hematocrit in the groups exposed to UVA compared to the control groups. Exposure to UVA induced marked red cell shrinkage (increased mean cell hemoglobin concentration) and showed an elevation in mean cell volume and mean cell hemoglobin in the blood of the exposed fish compared to the control. A significant (P<0.05) reduction in the total white blood cells was recorded in the exposed fish compared to the control. The biochemical parameters (blood glucose, total plasma protein, blood cholesterol, plasma creatinine, aspartic amino transferase and alanine amino transferase) exhibited a significant increase in the blood of fish exposed to UVA. Methanolic extract of quince leaf before ripening of the fruits was analyzed by GC/MS. To investigate the biological impact of this extract and its biologically active components, this extract was tested for its putative role in alleviation of UVA effect on catfish. Quince leaf extract had the ability to prevent hematotoxic stress induced by UVA and resulted in enhancement of the immune system of catfish represented by significant (P<0.05) increase in the number of white blood cells and lymphocytes of the catfish. Quince extract also protected the red blood cells from UVA damage. To our knowledge this is the first report of the effect of quince leaf extract on an aquatic organism.

Authors+Show Affiliations

Zoology Department, Faculty of Science, Al-Azhar University, 71524 Assiut, Egypt. agosman2@yahoo.comNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20206545

Citation

Osman, Alaa G M., et al. "Use of Hematological Parameters to Assess the Efficiency of Quince (Cydonia Oblonga Miller) Leaf Extract in Alleviation of the Effect of ultraviolet--A Radiation On African Catfish Clarias Gariepinus (Burchell, 1822)." Journal of Photochemistry and Photobiology. B, Biology, vol. 99, no. 1, 2010, pp. 1-8.
Osman AG, Koutb M, Sayed Ael-D. Use of hematological parameters to assess the efficiency of quince (Cydonia oblonga Miller) leaf extract in alleviation of the effect of ultraviolet--A radiation on African catfish Clarias gariepinus (Burchell, 1822). J Photochem Photobiol B. 2010;99(1):1-8.
Osman, A. G., Koutb, M., & Sayed, A. e. l. -. D. (2010). Use of hematological parameters to assess the efficiency of quince (Cydonia oblonga Miller) leaf extract in alleviation of the effect of ultraviolet--A radiation on African catfish Clarias gariepinus (Burchell, 1822). Journal of Photochemistry and Photobiology. B, Biology, 99(1), 1-8. https://doi.org/10.1016/j.jphotobiol.2010.01.002
Osman AG, Koutb M, Sayed Ael-D. Use of Hematological Parameters to Assess the Efficiency of Quince (Cydonia Oblonga Miller) Leaf Extract in Alleviation of the Effect of ultraviolet--A Radiation On African Catfish Clarias Gariepinus (Burchell, 1822). J Photochem Photobiol B. 2010 Apr 2;99(1):1-8. PubMed PMID: 20206545.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Use of hematological parameters to assess the efficiency of quince (Cydonia oblonga Miller) leaf extract in alleviation of the effect of ultraviolet--A radiation on African catfish Clarias gariepinus (Burchell, 1822). AU - Osman,Alaa G M, AU - Koutb,Mostafa, AU - Sayed,Alaa El-Din H, Y1 - 2010/01/25/ PY - 2009/11/08/received PY - 2009/12/30/revised PY - 2010/01/06/accepted PY - 2010/3/9/entrez PY - 2010/3/9/pubmed PY - 2010/6/4/medline SP - 1 EP - 8 JF - Journal of photochemistry and photobiology. B, Biology JO - J Photochem Photobiol B VL - 99 IS - 1 N2 - The present study aimed to elucidate the negative impacts of UVA on some biochemical and hematological variables of the economically important African catfish, Clarias gariepinus and investigates the putative role of quince (Cydonia oblonga Miller) leaf extract in protection and/or alleviation of such negative impacts. Changes in the hematological and blood biochemical values often reflect alteration of physiological state. Blood parameters can be useful for the measurement of physiological disturbances in stressed fish and thus provide information about the level of damage in the fish. We found a significant (P<0.05) decrease in the red blood cell counts, hemoglobin and hematocrit in the groups exposed to UVA compared to the control groups. Exposure to UVA induced marked red cell shrinkage (increased mean cell hemoglobin concentration) and showed an elevation in mean cell volume and mean cell hemoglobin in the blood of the exposed fish compared to the control. A significant (P<0.05) reduction in the total white blood cells was recorded in the exposed fish compared to the control. The biochemical parameters (blood glucose, total plasma protein, blood cholesterol, plasma creatinine, aspartic amino transferase and alanine amino transferase) exhibited a significant increase in the blood of fish exposed to UVA. Methanolic extract of quince leaf before ripening of the fruits was analyzed by GC/MS. To investigate the biological impact of this extract and its biologically active components, this extract was tested for its putative role in alleviation of UVA effect on catfish. Quince leaf extract had the ability to prevent hematotoxic stress induced by UVA and resulted in enhancement of the immune system of catfish represented by significant (P<0.05) increase in the number of white blood cells and lymphocytes of the catfish. Quince extract also protected the red blood cells from UVA damage. To our knowledge this is the first report of the effect of quince leaf extract on an aquatic organism. SN - 1873-2682 UR - https://www.unboundmedicine.com/medline/citation/20206545/Use_of_hematological_parameters_to_assess_the_efficiency_of_quince__Cydonia_oblonga_Miller__leaf_extract_in_alleviation_of_the_effect_of_ultraviolet__A_radiation_on_African_catfish_Clarias_gariepinus__Burchell_1822__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1011-1344(10)00004-7 DB - PRIME DP - Unbound Medicine ER -