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Hypoglycemic activity of leaf organic extracts from Smallanthus sonchifolius: Constituents of the most active fractions.
Chem Biol Interact. 2010 Apr 29; 185(2):143-52.CB

Abstract

The aim of the present study was to determine the in vivo hypoglycemic activity of five organic extracts and enhydrin obtained from yacon leaves. The main constituents of the most active fraction were identified. Five organic extracts and pure crystalline enhydrin were administered to normoglycemic, transiently hyperglycemic and streptozotocin (STZ)-diabetic rats. The fasting and post-prandial blood glucose, and serum insulin levels were estimated and an oral glucose tolerance test (OGTT) was performed for the evaluation of hypoglycemic activity and dose optimization of each extract. We found that the methanol, butanol and chloroform extracts showed effective hypoglycemic activity at minimum doses of 50, 10 and 20mg/kg body weight, respectively, and were selected for further experiments. Oral administration of a single-dose of each extract produced a slight lowering effect in the fasting blood glucose level of normal healthy rats, whereas each extract tempered significantly the hyperglycemic peak after food ingestion. Daily administration of each extract for 8 weeks produced an effective glycemic control in diabetic animals with an increase in the plasma insulin level. Phytochemical analysis of the most active fraction, the butanol extract, showed that caffeic, chlorogenic and three dicaffeoilquinic acids were significant components. Additionally, enhydrin, the major sesquiterpene lactone of yacon leaves, was also effective to reduce post-prandial glucose and useful in the treatment of diabetic animals (minimum dose: 0.8mg/kg body weight). The results presented here strongly support the notion that the phenolic compounds above as well as enhydrin are important hypoglycemic principles of yacon leaves that could ameliorate the diabetic state.

Authors+Show Affiliations

Instituto Superior de Investigaciones Biológicas, Universidad Nacional de Tucumán, San Miguel de Tucumán, Argentina.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20211156

Citation

Genta, Susana B., et al. "Hypoglycemic Activity of Leaf Organic Extracts From Smallanthus Sonchifolius: Constituents of the Most Active Fractions." Chemico-biological Interactions, vol. 185, no. 2, 2010, pp. 143-52.
Genta SB, Cabrera WM, Mercado MI, et al. Hypoglycemic activity of leaf organic extracts from Smallanthus sonchifolius: Constituents of the most active fractions. Chem Biol Interact. 2010;185(2):143-52.
Genta, S. B., Cabrera, W. M., Mercado, M. I., Grau, A., Catalán, C. A., & Sánchez, S. S. (2010). Hypoglycemic activity of leaf organic extracts from Smallanthus sonchifolius: Constituents of the most active fractions. Chemico-biological Interactions, 185(2), 143-52. https://doi.org/10.1016/j.cbi.2010.03.004
Genta SB, et al. Hypoglycemic Activity of Leaf Organic Extracts From Smallanthus Sonchifolius: Constituents of the Most Active Fractions. Chem Biol Interact. 2010 Apr 29;185(2):143-52. PubMed PMID: 20211156.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hypoglycemic activity of leaf organic extracts from Smallanthus sonchifolius: Constituents of the most active fractions. AU - Genta,Susana B, AU - Cabrera,Wilfredo M, AU - Mercado,María I, AU - Grau,Alfredo, AU - Catalán,César A, AU - Sánchez,Sara S, Y1 - 2010/03/06/ PY - 2009/12/23/received PY - 2010/02/25/revised PY - 2010/03/01/accepted PY - 2010/3/10/entrez PY - 2010/3/10/pubmed PY - 2010/5/11/medline SP - 143 EP - 52 JF - Chemico-biological interactions JO - Chem Biol Interact VL - 185 IS - 2 N2 - The aim of the present study was to determine the in vivo hypoglycemic activity of five organic extracts and enhydrin obtained from yacon leaves. The main constituents of the most active fraction were identified. Five organic extracts and pure crystalline enhydrin were administered to normoglycemic, transiently hyperglycemic and streptozotocin (STZ)-diabetic rats. The fasting and post-prandial blood glucose, and serum insulin levels were estimated and an oral glucose tolerance test (OGTT) was performed for the evaluation of hypoglycemic activity and dose optimization of each extract. We found that the methanol, butanol and chloroform extracts showed effective hypoglycemic activity at minimum doses of 50, 10 and 20mg/kg body weight, respectively, and were selected for further experiments. Oral administration of a single-dose of each extract produced a slight lowering effect in the fasting blood glucose level of normal healthy rats, whereas each extract tempered significantly the hyperglycemic peak after food ingestion. Daily administration of each extract for 8 weeks produced an effective glycemic control in diabetic animals with an increase in the plasma insulin level. Phytochemical analysis of the most active fraction, the butanol extract, showed that caffeic, chlorogenic and three dicaffeoilquinic acids were significant components. Additionally, enhydrin, the major sesquiterpene lactone of yacon leaves, was also effective to reduce post-prandial glucose and useful in the treatment of diabetic animals (minimum dose: 0.8mg/kg body weight). The results presented here strongly support the notion that the phenolic compounds above as well as enhydrin are important hypoglycemic principles of yacon leaves that could ameliorate the diabetic state. SN - 1872-7786 UR - https://www.unboundmedicine.com/medline/citation/20211156/Hypoglycemic_activity_of_leaf_organic_extracts_from_Smallanthus_sonchifolius:_Constituents_of_the_most_active_fractions_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0009-2797(10)00117-1 DB - PRIME DP - Unbound Medicine ER -