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Selection and identification of B-cell epitope on NS1 protein of dengue virus type 2.
Virus Res. 2010 Jun; 150(1-2):49-55.VR

Abstract

NS1 of dengue virus (DENV) is an important non-structural protein, which plays an important role in DENV replication and dengue infection. In this study, using the phage-displayed peptide library screening method and purified anti-DENV2-NS1 polyclonal antibody immunoglobulin G (IgG) as target, which was generated from the purified recombinant expressed DENV2-NS1 protein immunization on rabbit, seven B-cell epitopes of DENV2-NS1 protein were screened. Considering the results of comprehensive bioinformatic analysis on NS1 B-cell epitopes, possible dominant B-cell epitopes are located in amino acids residues 36-45, 80-89, 103-112, 121-130, 187-196, 295-304, and 315-324 of the NS1, and two epitope-based NS1 protein dodecapeptides corresponding to the predominant epitopes (PA10: (36)PESPSKLASA(45) and AA10: (187)AIKDNRAVHA(196)) were chosen for synthesis. Results of binding assay and competitive-inhibition assays indicated the two peptides were the specific epitopes of DENV2-NS1 protein. These epitopes could be useful in understanding the pathogenesis of DENV and as dengue vaccine constituents in further study.

Authors+Show Affiliations

Department of Microbiology, Zhongshan School of Medicine, Sun Yat-Sen University, Zhongshan Road 2, Guangzhou 510080, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20211209

Citation

Jiang, Lan, et al. "Selection and Identification of B-cell Epitope On NS1 Protein of Dengue Virus Type 2." Virus Research, vol. 150, no. 1-2, 2010, pp. 49-55.
Jiang L, Zhou JM, Yin Y, et al. Selection and identification of B-cell epitope on NS1 protein of dengue virus type 2. Virus Res. 2010;150(1-2):49-55.
Jiang, L., Zhou, J. M., Yin, Y., Fang, D. Y., Tang, Y. X., & Jiang, L. F. (2010). Selection and identification of B-cell epitope on NS1 protein of dengue virus type 2. Virus Research, 150(1-2), 49-55. https://doi.org/10.1016/j.virusres.2010.02.012
Jiang L, et al. Selection and Identification of B-cell Epitope On NS1 Protein of Dengue Virus Type 2. Virus Res. 2010;150(1-2):49-55. PubMed PMID: 20211209.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Selection and identification of B-cell epitope on NS1 protein of dengue virus type 2. AU - Jiang,Lan, AU - Zhou,Jun-Mei, AU - Yin,Yue, AU - Fang,Dan-Yun, AU - Tang,Yun-Xia, AU - Jiang,Li-Fang, Y1 - 2010/03/06/ PY - 2009/10/22/received PY - 2010/02/23/revised PY - 2010/02/24/accepted PY - 2010/3/10/entrez PY - 2010/3/10/pubmed PY - 2010/7/20/medline SP - 49 EP - 55 JF - Virus research JO - Virus Res VL - 150 IS - 1-2 N2 - NS1 of dengue virus (DENV) is an important non-structural protein, which plays an important role in DENV replication and dengue infection. In this study, using the phage-displayed peptide library screening method and purified anti-DENV2-NS1 polyclonal antibody immunoglobulin G (IgG) as target, which was generated from the purified recombinant expressed DENV2-NS1 protein immunization on rabbit, seven B-cell epitopes of DENV2-NS1 protein were screened. Considering the results of comprehensive bioinformatic analysis on NS1 B-cell epitopes, possible dominant B-cell epitopes are located in amino acids residues 36-45, 80-89, 103-112, 121-130, 187-196, 295-304, and 315-324 of the NS1, and two epitope-based NS1 protein dodecapeptides corresponding to the predominant epitopes (PA10: (36)PESPSKLASA(45) and AA10: (187)AIKDNRAVHA(196)) were chosen for synthesis. Results of binding assay and competitive-inhibition assays indicated the two peptides were the specific epitopes of DENV2-NS1 protein. These epitopes could be useful in understanding the pathogenesis of DENV and as dengue vaccine constituents in further study. SN - 1872-7492 UR - https://www.unboundmedicine.com/medline/citation/20211209/Selection_and_identification_of_B_cell_epitope_on_NS1_protein_of_dengue_virus_type_2_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0168-1702(10)00079-1 DB - PRIME DP - Unbound Medicine ER -