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Differential accumulation of phytohormones in wheat seedlings attacked by avirulent and virulent Hessian fly (Diptera: Cecidomyiidae) larvae.
J Econ Entomol. 2010 Feb; 103(1):178-85.JE

Abstract

We analyzed the accumulation of six phytohormones and phytohormone-related compounds in a wheat, Triticum aestivium L., genotype, 'Molly', after attacks by avirulent and virulent Hessian fly, Mayetiola destructor (Say) (Diptera: Cecidomyiidae), larvae, respectively, and we examined the expression of genes in the jasmonic acid (JA) pathway by Northern blot analysis. Compared with uninfested plants, attacks by avirulent larvae resulted in increased accumulation of salicylic acid (SA) by 11.3- and 8.2-fold, 12-oxo-phytodienoic acid (OPDA) by 36.4-and 18.7-fold, 18:3 fatty acid by 4.5- and 2.2-fold, and 18:1 fatty acid by 1.8- and 1.9-fold at 24 and 72 h post-initial attack (hpia), respectively, but an 20% decrease in JA accumulation at 24 hpia at the attack site. Attacks by the virulent larvae did not affect the accumulation of SA, OPDA, and 18:3 and 18:1 fatty acids but dramatically increased the concentration of auxin (AUX) from undetectable in uninfested plants to 381.7 ng/g fresh weight at 24 hpia and 71.0 ng/g fresh weight at 72 hpia in infested plants. Transcript levels of genes encoding lipoxygenase 2, allene oxide synthase, and Arabidopsis storage protein 2 were increased after avirulent larval attacks but decreased after virulent larval attacks. Our results suggest that OPDA and SA may act together in wheat resistance to the Hessian fly, whereas AUX may play a role in the susceptibility of wheat plants. The increased OPDA accumulation after avirulent larval attacks was at least partially regulated through gene transcription.

Authors+Show Affiliations

Department of Natural Science, Fayetteville State University, Fayetteville, NC 28301, USA. lzhu@uncfsu.eduNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

20214384

Citation

Zhu, Lieceng, et al. "Differential Accumulation of Phytohormones in Wheat Seedlings Attacked By Avirulent and Virulent Hessian Fly (Diptera: Cecidomyiidae) Larvae." Journal of Economic Entomology, vol. 103, no. 1, 2010, pp. 178-85.
Zhu L, Liu X, Chen MS. Differential accumulation of phytohormones in wheat seedlings attacked by avirulent and virulent Hessian fly (Diptera: Cecidomyiidae) larvae. J Econ Entomol. 2010;103(1):178-85.
Zhu, L., Liu, X., & Chen, M. S. (2010). Differential accumulation of phytohormones in wheat seedlings attacked by avirulent and virulent Hessian fly (Diptera: Cecidomyiidae) larvae. Journal of Economic Entomology, 103(1), 178-85.
Zhu L, Liu X, Chen MS. Differential Accumulation of Phytohormones in Wheat Seedlings Attacked By Avirulent and Virulent Hessian Fly (Diptera: Cecidomyiidae) Larvae. J Econ Entomol. 2010;103(1):178-85. PubMed PMID: 20214384.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential accumulation of phytohormones in wheat seedlings attacked by avirulent and virulent Hessian fly (Diptera: Cecidomyiidae) larvae. AU - Zhu,Lieceng, AU - Liu,Xiang, AU - Chen,Ming-Shun, PY - 2010/3/11/entrez PY - 2010/3/11/pubmed PY - 2010/4/7/medline SP - 178 EP - 85 JF - Journal of economic entomology JO - J Econ Entomol VL - 103 IS - 1 N2 - We analyzed the accumulation of six phytohormones and phytohormone-related compounds in a wheat, Triticum aestivium L., genotype, 'Molly', after attacks by avirulent and virulent Hessian fly, Mayetiola destructor (Say) (Diptera: Cecidomyiidae), larvae, respectively, and we examined the expression of genes in the jasmonic acid (JA) pathway by Northern blot analysis. Compared with uninfested plants, attacks by avirulent larvae resulted in increased accumulation of salicylic acid (SA) by 11.3- and 8.2-fold, 12-oxo-phytodienoic acid (OPDA) by 36.4-and 18.7-fold, 18:3 fatty acid by 4.5- and 2.2-fold, and 18:1 fatty acid by 1.8- and 1.9-fold at 24 and 72 h post-initial attack (hpia), respectively, but an 20% decrease in JA accumulation at 24 hpia at the attack site. Attacks by the virulent larvae did not affect the accumulation of SA, OPDA, and 18:3 and 18:1 fatty acids but dramatically increased the concentration of auxin (AUX) from undetectable in uninfested plants to 381.7 ng/g fresh weight at 24 hpia and 71.0 ng/g fresh weight at 72 hpia in infested plants. Transcript levels of genes encoding lipoxygenase 2, allene oxide synthase, and Arabidopsis storage protein 2 were increased after avirulent larval attacks but decreased after virulent larval attacks. Our results suggest that OPDA and SA may act together in wheat resistance to the Hessian fly, whereas AUX may play a role in the susceptibility of wheat plants. The increased OPDA accumulation after avirulent larval attacks was at least partially regulated through gene transcription. SN - 0022-0493 UR - https://www.unboundmedicine.com/medline/citation/20214384/Differential_accumulation_of_phytohormones_in_wheat_seedlings_attacked_by_avirulent_and_virulent_Hessian_fly__Diptera:_Cecidomyiidae__larvae_ L2 - https://academic.oup.com/jee/article-lookup/doi/10.1603/ec09224 DB - PRIME DP - Unbound Medicine ER -