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Limb remote ischemic preconditioning protects the spinal cord from ischemia-reperfusion injury: a newly identified nonneuronal but reactive oxygen species-dependent pathway.
Anesthesiology. 2010 Apr; 112(4):881-91.A

Abstract

BACKGROUND

It remains to be established whether spinal cord ischemic tolerance can be induced by limb remote ischemic preconditioning (RIPC), and the mechanisms underlying the neuroprotective effects of RIPC on the spinal cord need to be clarified.

METHODS

Spinal cord ischemia was studied in New Zealand White rabbits. In experiment 1, all rabbits were subjected to 20-min spinal cord ischemia by aortic occlusion. Thirty minutes before ischemia, rabbits were subjected to sham intervention or RIPC achieved by bilateral femoral artery occlusion (10 min ischemia/10 min reperfusion, two cycles). Dimethylthiourea (500 mg/kg, intravenously), a hydroxyl radical scavenger, or vehicle was given 1 h before RIPC. Antioxidant enzyme activity was measured along with spinal cord histology and neurologic function. In experiment 2, rabbits were subjected to spinal cord ischemia, with or without RIPC. In addition, rabbits were pretreated with various doses of hexamethonium.

RESULTS

RIPC improved neurologic function and reduced histologic damage. This was associated with increased endogenous antioxidant activity. Dimethylthiourea inhibited the protective effects of RIPC. In contrast, there was no effect of hexamethonium on the protective effect of RIPC.

CONCLUSIONS

An initial oxidative stress acts as a trigger to upregulate antioxidant enzyme activity, rather than the neural pathway, and plays an important role in the formation of the tolerance against spinal cord ischemia by limb RIPC.

Authors+Show Affiliations

Department of Anesthesiology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20216397

Citation

Dong, Hai-Long, et al. "Limb Remote Ischemic Preconditioning Protects the Spinal Cord From Ischemia-reperfusion Injury: a Newly Identified Nonneuronal but Reactive Oxygen Species-dependent Pathway." Anesthesiology, vol. 112, no. 4, 2010, pp. 881-91.
Dong HL, Zhang Y, Su BX, et al. Limb remote ischemic preconditioning protects the spinal cord from ischemia-reperfusion injury: a newly identified nonneuronal but reactive oxygen species-dependent pathway. Anesthesiology. 2010;112(4):881-91.
Dong, H. L., Zhang, Y., Su, B. X., Zhu, Z. H., Gu, Q. H., Sang, H. F., & Xiong, L. (2010). Limb remote ischemic preconditioning protects the spinal cord from ischemia-reperfusion injury: a newly identified nonneuronal but reactive oxygen species-dependent pathway. Anesthesiology, 112(4), 881-91. https://doi.org/10.1097/ALN.0b013e3181d0486d
Dong HL, et al. Limb Remote Ischemic Preconditioning Protects the Spinal Cord From Ischemia-reperfusion Injury: a Newly Identified Nonneuronal but Reactive Oxygen Species-dependent Pathway. Anesthesiology. 2010;112(4):881-91. PubMed PMID: 20216397.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Limb remote ischemic preconditioning protects the spinal cord from ischemia-reperfusion injury: a newly identified nonneuronal but reactive oxygen species-dependent pathway. AU - Dong,Hai-Long, AU - Zhang,Yi, AU - Su,Bin-Xiao, AU - Zhu,Zheng-Hua, AU - Gu,Qiu-Han, AU - Sang,Han-Fei, AU - Xiong,Lize, PY - 2010/3/11/entrez PY - 2010/3/11/pubmed PY - 2010/5/5/medline SP - 881 EP - 91 JF - Anesthesiology JO - Anesthesiology VL - 112 IS - 4 N2 - BACKGROUND: It remains to be established whether spinal cord ischemic tolerance can be induced by limb remote ischemic preconditioning (RIPC), and the mechanisms underlying the neuroprotective effects of RIPC on the spinal cord need to be clarified. METHODS: Spinal cord ischemia was studied in New Zealand White rabbits. In experiment 1, all rabbits were subjected to 20-min spinal cord ischemia by aortic occlusion. Thirty minutes before ischemia, rabbits were subjected to sham intervention or RIPC achieved by bilateral femoral artery occlusion (10 min ischemia/10 min reperfusion, two cycles). Dimethylthiourea (500 mg/kg, intravenously), a hydroxyl radical scavenger, or vehicle was given 1 h before RIPC. Antioxidant enzyme activity was measured along with spinal cord histology and neurologic function. In experiment 2, rabbits were subjected to spinal cord ischemia, with or without RIPC. In addition, rabbits were pretreated with various doses of hexamethonium. RESULTS: RIPC improved neurologic function and reduced histologic damage. This was associated with increased endogenous antioxidant activity. Dimethylthiourea inhibited the protective effects of RIPC. In contrast, there was no effect of hexamethonium on the protective effect of RIPC. CONCLUSIONS: An initial oxidative stress acts as a trigger to upregulate antioxidant enzyme activity, rather than the neural pathway, and plays an important role in the formation of the tolerance against spinal cord ischemia by limb RIPC. SN - 1528-1175 UR - https://www.unboundmedicine.com/medline/citation/20216397/Limb_remote_ischemic_preconditioning_protects_the_spinal_cord_from_ischemia_reperfusion_injury:_a_newly_identified_nonneuronal_but_reactive_oxygen_species_dependent_pathway_ L2 - http://anesthesiology.pubs.asahq.org/article.aspx?doi=10.1097/ALN.0b013e3181d0486d DB - PRIME DP - Unbound Medicine ER -