Tags

Type your tag names separated by a space and hit enter

Identification of SARS-like coronaviruses in horseshoe bats (Rhinolophus hipposideros) in Slovenia.
Arch Virol. 2010 Apr; 155(4):507-14.AV

Abstract

Bats have been identified as a natural reservoir for an increasing number of emerging zoonotic viruses, such as Hendra virus, Nipah virus, Ebola virus, Marburg virus, rabies and other lyssaviruses. Recently, a large number of viruses closely related to members of the genus Coronavirus have been associated with severe acute respiratory syndrome (SARS) and detected in bat species. In this study, samples were collected from 106 live bats of seven different bat species from 27 different locations in Slovenia. Coronaviruses were detected by RT-PCR in 14 out of 36 horseshoe bat (Rhinolophus hipposideros) fecal samples, with 38.8% virus prevalence. Sequence analysis of a 405-nucleotide region of the highly conserved RNA polymerase gene (pol) showed that all coronaviruses detected in this study are genetically closely related, with 99.5-100% nucleotide identity, and belong to group 2 of the coronaviruses. The most closely related virus sequence in GenBank was SARS bat isolate Rp3/2004 (DQ071615) within the SARS-like CoV cluster, sharing 85% nucleotide identity and 95.6% amino acid identity. The potential risk of a new group of bat coronaviruses as a reservoir for human infections is highly suspected, and further molecular epidemiologic studies of these bat coronaviruses are needed.

Links

PMC Free PDF
PMC Free Full Text

Authors+Show Affiliations

Rihtaric D
Virology Unit, Veterinary Faculty, Institute of Microbiology and Parasitology, University of Ljubljana, Gerbiceva 60, 1115 Ljubljana, Slovenia.
Hostnik P
No affiliation info available
Steyer A
No affiliation info available
Grom J
No affiliation info available
Toplak I
No affiliation info available

MeSH

AnimalsChiropteraCluster AnalysisCoronavirusCoronavirus InfectionsFecesGene Products, polMolecular Sequence DataPhylogenyPrevalenceRNA, ViralReverse Transcriptase Polymerase Chain ReactionSequence Analysis, DNASequence HomologySlovenia

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20217155

Citation

Rihtaric, Danijela, et al. "Identification of SARS-like Coronaviruses in Horseshoe Bats (Rhinolophus Hipposideros) in Slovenia." Archives of Virology, vol. 155, no. 4, 2010, pp. 507-14.
Rihtaric D, Hostnik P, Steyer A, et al. Identification of SARS-like coronaviruses in horseshoe bats (Rhinolophus hipposideros) in Slovenia. Arch Virol. 2010;155(4):507-14.
Rihtaric, D., Hostnik, P., Steyer, A., Grom, J., & Toplak, I. (2010). Identification of SARS-like coronaviruses in horseshoe bats (Rhinolophus hipposideros) in Slovenia. Archives of Virology, 155(4), 507-14. https://doi.org/10.1007/s00705-010-0612-5
Rihtaric D, et al. Identification of SARS-like Coronaviruses in Horseshoe Bats (Rhinolophus Hipposideros) in Slovenia. Arch Virol. 2010;155(4):507-14. PubMed PMID: 20217155.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of SARS-like coronaviruses in horseshoe bats (Rhinolophus hipposideros) in Slovenia. AU - Rihtaric,Danijela, AU - Hostnik,Peter, AU - Steyer,Andrej, AU - Grom,Joze, AU - Toplak,Ivan, Y1 - 2010/03/10/ PY - 2009/09/22/received PY - 2010/01/04/accepted PY - 2010/3/11/entrez PY - 2010/3/11/pubmed PY - 2010/4/14/medline SP - 507 EP - 14 JF - Archives of virology JO - Arch Virol VL - 155 IS - 4 N2 - Bats have been identified as a natural reservoir for an increasing number of emerging zoonotic viruses, such as Hendra virus, Nipah virus, Ebola virus, Marburg virus, rabies and other lyssaviruses. Recently, a large number of viruses closely related to members of the genus Coronavirus have been associated with severe acute respiratory syndrome (SARS) and detected in bat species. In this study, samples were collected from 106 live bats of seven different bat species from 27 different locations in Slovenia. Coronaviruses were detected by RT-PCR in 14 out of 36 horseshoe bat (Rhinolophus hipposideros) fecal samples, with 38.8% virus prevalence. Sequence analysis of a 405-nucleotide region of the highly conserved RNA polymerase gene (pol) showed that all coronaviruses detected in this study are genetically closely related, with 99.5-100% nucleotide identity, and belong to group 2 of the coronaviruses. The most closely related virus sequence in GenBank was SARS bat isolate Rp3/2004 (DQ071615) within the SARS-like CoV cluster, sharing 85% nucleotide identity and 95.6% amino acid identity. The potential risk of a new group of bat coronaviruses as a reservoir for human infections is highly suspected, and further molecular epidemiologic studies of these bat coronaviruses are needed. SN - 1432-8798 UR - https://www.unboundmedicine.com/medline/citation/20217155/Identification_of_SARS_like_coronaviruses_in_horseshoe_bats__Rhinolophus_hipposideros__in_Slovenia_ L2 - https://dx.doi.org/10.1007/s00705-010-0612-5 DB - PRIME DP - Unbound Medicine ER -