Tags

Type your tag names separated by a space and hit enter

Effects of polyphenol-rich extract from berries of Aronia melanocarpa on the markers of oxidative stress and blood platelet activation.
Platelets 2010; 21(4):274-81P

Abstract

Bioactive substances found in numerous foods can be successfully and safely used to modify various cellular functions and affect the oxidative stress. Aronia melanocarpa fruits (Rosaceae) are one of the richest plant sources of phenolic substances shown to have anti-inflammatory, antitumor, antioxidative and antiplatelet activities. We investigated antioxidant properties of the extract from berries of A. melanocarpa by the estimation of the selected and other biomarkers of oxidative stress, i.e. the level of 8-epi-prostaglandin F(2) (8-EPI) (by immunoassay kit) and the amount of glutathione (by HPLC method) in control platelets and platelets treated with H(2)O(2). The expression of alpha(IIb)beta(3) (a marker of platelet activation) was measured by flow cytometer. The antioxidative and antiplatelet properties of the tested extract were also compared with the action of a well characterized antioxidative and antiplatelet commercial monomeric polyphenol-resveratrol. The extract from berries of A. melanocarpa (at the highest tested concentration -100 microg/ml) decreased the production of 8-EPI (a marker of lipid peroxidation) in control blood platelets and platelets treated with H(2)O(2) (2 mM). A combined action of the tested plant extract and H(2)O(2) evoked a significant increase of reduced form of glutathione in platelets compared with cells treated with H(2)O(2) only. Moreover, the tested plant extract (at the highest used concentration -100 microg/ml) reduced the expression of alpha(IIb)beta(3) on blood platelets. Comparative studies indicate that the tested plant extract was found to be more reactive in blood platelets than the solution of pure resveratrol.

Authors+Show Affiliations

Department of General Biochemistry, Institute of Biochemistry, University of Lodz, Lodz, Poland. olasb@biol.uni.lodz.plNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20218910

Citation

Olas, Beata, et al. "Effects of Polyphenol-rich Extract From Berries of Aronia Melanocarpa On the Markers of Oxidative Stress and Blood Platelet Activation." Platelets, vol. 21, no. 4, 2010, pp. 274-81.
Olas B, Kedzierska M, Wachowicz B, et al. Effects of polyphenol-rich extract from berries of Aronia melanocarpa on the markers of oxidative stress and blood platelet activation. Platelets. 2010;21(4):274-81.
Olas, B., Kedzierska, M., Wachowicz, B., Stochmal, A., & Oleszek, W. (2010). Effects of polyphenol-rich extract from berries of Aronia melanocarpa on the markers of oxidative stress and blood platelet activation. Platelets, 21(4), pp. 274-81. doi:10.3109/09537101003612821.
Olas B, et al. Effects of Polyphenol-rich Extract From Berries of Aronia Melanocarpa On the Markers of Oxidative Stress and Blood Platelet Activation. Platelets. 2010;21(4):274-81. PubMed PMID: 20218910.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of polyphenol-rich extract from berries of Aronia melanocarpa on the markers of oxidative stress and blood platelet activation. AU - Olas,Beata, AU - Kedzierska,Magdalena, AU - Wachowicz,Barbara, AU - Stochmal,Anna, AU - Oleszek,Wieslaw, PY - 2010/3/12/entrez PY - 2010/3/12/pubmed PY - 2010/8/13/medline SP - 274 EP - 81 JF - Platelets JO - Platelets VL - 21 IS - 4 N2 - Bioactive substances found in numerous foods can be successfully and safely used to modify various cellular functions and affect the oxidative stress. Aronia melanocarpa fruits (Rosaceae) are one of the richest plant sources of phenolic substances shown to have anti-inflammatory, antitumor, antioxidative and antiplatelet activities. We investigated antioxidant properties of the extract from berries of A. melanocarpa by the estimation of the selected and other biomarkers of oxidative stress, i.e. the level of 8-epi-prostaglandin F(2) (8-EPI) (by immunoassay kit) and the amount of glutathione (by HPLC method) in control platelets and platelets treated with H(2)O(2). The expression of alpha(IIb)beta(3) (a marker of platelet activation) was measured by flow cytometer. The antioxidative and antiplatelet properties of the tested extract were also compared with the action of a well characterized antioxidative and antiplatelet commercial monomeric polyphenol-resveratrol. The extract from berries of A. melanocarpa (at the highest tested concentration -100 microg/ml) decreased the production of 8-EPI (a marker of lipid peroxidation) in control blood platelets and platelets treated with H(2)O(2) (2 mM). A combined action of the tested plant extract and H(2)O(2) evoked a significant increase of reduced form of glutathione in platelets compared with cells treated with H(2)O(2) only. Moreover, the tested plant extract (at the highest used concentration -100 microg/ml) reduced the expression of alpha(IIb)beta(3) on blood platelets. Comparative studies indicate that the tested plant extract was found to be more reactive in blood platelets than the solution of pure resveratrol. SN - 1369-1635 UR - https://www.unboundmedicine.com/medline/citation/20218910/Effects_of_polyphenol_rich_extract_from_berries_of_Aronia_melanocarpa_on_the_markers_of_oxidative_stress_and_blood_platelet_activation_ L2 - http://www.tandfonline.com/doi/full/10.3109/09537101003612821 DB - PRIME DP - Unbound Medicine ER -