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Epidemiology and molecular characteristics of community-associated methicillin-resistant and methicillin-susceptible Staphylococcus aureus from skin/soft tissue infections in a children's hospital in Beijing, China.
Diagn Microbiol Infect Dis 2010; 67(1):1-8DM

Abstract

To evaluate the epidemiology and molecular features of community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) and methicillin-sensitive S. aureus (MSSA) from children with skin and soft tissue infections (SSTIs) in Beijing, China, prospective community-acquired S. aureus SSTIs surveillance was conducted at the Beijing Children's Hospital, Beijing, China, for a 12-month period from August 1, 2008, to July 30, 2009. Susceptibility to 12 antimicrobials was determined by the agar dilution method. Genotypic characteristics of CA-MRSA isolates were tested by SCCmec typing, spa typing, and multilocus sequence typing. Panton-Valentine leukocidin gene was detected. Of 1104 cases, 31.8% (351) were community-acquired S. aureus. CA-MRSA accounted for 4% (14) of S. aureus. Among 14 CA-MRSA and 120 MSSA isolates tested, 100% and 91.7% were multidrug resistant, respectively. ST59-MRSA-IVa-t437 (42.9%) was the most common form of CA-MRSA. Spa typing analysis of 120 MSSA isolates was performed, followed by pulsed-field gel electrophoresis and multilocus sequence typing of a selected number of isolates. The most common spa types among MSSA were t084 (8.3%), t091 (5.8%), t034 (5%), t127 (4.2%), t002 (4.2%), and t796 (4.2%). No predominant spa type was seen. Of the MSSA isolates that could be classified into spa-CCs, 15.0% had a genetic background observed in CA-MRSA clones (spa-CC437, spa-CC342, and spa-CC377). Panton-Valentine Leukocidin (PVL)-positive community-acquired S. aureus strains were more commonly associated with skin abscesses than other SSTIs (29.4% versus 5.9%, P < 0.01).In conclusion, CA-MRSA infections are not common among Chinese children with SSTIs. Our findings show that MSSA strains in China have diverse genetic backgrounds.

Authors+Show Affiliations

Beijing Children's Hospital, Capital Medical University, Beijing 100045, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20227225

Citation

Wu, Dejing, et al. "Epidemiology and Molecular Characteristics of Community-associated Methicillin-resistant and Methicillin-susceptible Staphylococcus Aureus From Skin/soft Tissue Infections in a Children's Hospital in Beijing, China." Diagnostic Microbiology and Infectious Disease, vol. 67, no. 1, 2010, pp. 1-8.
Wu D, Wang Q, Yang Y, et al. Epidemiology and molecular characteristics of community-associated methicillin-resistant and methicillin-susceptible Staphylococcus aureus from skin/soft tissue infections in a children's hospital in Beijing, China. Diagn Microbiol Infect Dis. 2010;67(1):1-8.
Wu, D., Wang, Q., Yang, Y., Geng, W., Wang, Q., Yu, S., ... Shen, X. (2010). Epidemiology and molecular characteristics of community-associated methicillin-resistant and methicillin-susceptible Staphylococcus aureus from skin/soft tissue infections in a children's hospital in Beijing, China. Diagnostic Microbiology and Infectious Disease, 67(1), pp. 1-8. doi:10.1016/j.diagmicrobio.2009.12.006.
Wu D, et al. Epidemiology and Molecular Characteristics of Community-associated Methicillin-resistant and Methicillin-susceptible Staphylococcus Aureus From Skin/soft Tissue Infections in a Children's Hospital in Beijing, China. Diagn Microbiol Infect Dis. 2010;67(1):1-8. PubMed PMID: 20227225.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Epidemiology and molecular characteristics of community-associated methicillin-resistant and methicillin-susceptible Staphylococcus aureus from skin/soft tissue infections in a children's hospital in Beijing, China. AU - Wu,Dejing, AU - Wang,Qun, AU - Yang,Yonghong, AU - Geng,Wenjing, AU - Wang,Qiang, AU - Yu,Sangjie, AU - Yao,Kaihu, AU - Yuan,Lin, AU - Shen,Xuzhuang, Y1 - 2010/03/12/ PY - 2009/08/26/received PY - 2009/11/27/revised PY - 2009/12/06/accepted PY - 2010/3/16/entrez PY - 2010/3/17/pubmed PY - 2010/6/22/medline SP - 1 EP - 8 JF - Diagnostic microbiology and infectious disease JO - Diagn. Microbiol. Infect. Dis. VL - 67 IS - 1 N2 - To evaluate the epidemiology and molecular features of community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) and methicillin-sensitive S. aureus (MSSA) from children with skin and soft tissue infections (SSTIs) in Beijing, China, prospective community-acquired S. aureus SSTIs surveillance was conducted at the Beijing Children's Hospital, Beijing, China, for a 12-month period from August 1, 2008, to July 30, 2009. Susceptibility to 12 antimicrobials was determined by the agar dilution method. Genotypic characteristics of CA-MRSA isolates were tested by SCCmec typing, spa typing, and multilocus sequence typing. Panton-Valentine leukocidin gene was detected. Of 1104 cases, 31.8% (351) were community-acquired S. aureus. CA-MRSA accounted for 4% (14) of S. aureus. Among 14 CA-MRSA and 120 MSSA isolates tested, 100% and 91.7% were multidrug resistant, respectively. ST59-MRSA-IVa-t437 (42.9%) was the most common form of CA-MRSA. Spa typing analysis of 120 MSSA isolates was performed, followed by pulsed-field gel electrophoresis and multilocus sequence typing of a selected number of isolates. The most common spa types among MSSA were t084 (8.3%), t091 (5.8%), t034 (5%), t127 (4.2%), t002 (4.2%), and t796 (4.2%). No predominant spa type was seen. Of the MSSA isolates that could be classified into spa-CCs, 15.0% had a genetic background observed in CA-MRSA clones (spa-CC437, spa-CC342, and spa-CC377). Panton-Valentine Leukocidin (PVL)-positive community-acquired S. aureus strains were more commonly associated with skin abscesses than other SSTIs (29.4% versus 5.9%, P < 0.01).In conclusion, CA-MRSA infections are not common among Chinese children with SSTIs. Our findings show that MSSA strains in China have diverse genetic backgrounds. SN - 1879-0070 UR - https://www.unboundmedicine.com/medline/citation/20227225/Epidemiology_and_molecular_characteristics_of_community_associated_methicillin_resistant_and_methicillin_susceptible_Staphylococcus_aureus_from_skin/soft_tissue_infections_in_a_children's_hospital_in_Beijing_China_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0732-8893(09)00483-0 DB - PRIME DP - Unbound Medicine ER -