TY - JOUR T1 - Determination of selenomethionine, selenocysteine, and inorganic selenium in eggs by HPLC-inductively coupled plasma mass spectrometry. AU - Lipiec,Elzbieta, AU - Siara,Grzegorz, AU - Bierla,Katarzyna, AU - Ouerdane,Laurent, AU - Szpunar,Joanna, Y1 - 2010/03/13/ PY - 2009/12/05/received PY - 2010/02/05/accepted PY - 2010/02/03/revised PY - 2010/3/16/entrez PY - 2010/3/17/pubmed PY - 2010/7/10/medline SP - 731 EP - 41 JF - Analytical and bioanalytical chemistry JO - Anal Bioanal Chem VL - 397 IS - 2 N2 - A method for the simultaneous determination of selenomethionine (SeMet), selenocysteine (SeCys), and selenite [Se(IV)] in chicken eggs was developed. A sample preparation protocol including defatting, protein denaturation, and carbamidomethylation was optimized in order to achieve complete protein digestion and to avoid SeCys losses. Quantification was carried out by reversed-phase HPLC-inductively coupled plasma mass spectrometry (ICP MS) after quantitative isolation of the selenium-containing fraction by size-exclusion liquid chromatography. The detection limits were 0.06, 0.003, and 0.01 microg g(-1) (dry weight) for SeCys, Se(IV) and SeMet, respectively, and the precision was 5-10%. The end products of carbamidomethylation of the different selenium species were identified for the first time by electrospray QTOF MS after custom-designed 2D HPLC purification. Differences in selenium speciation in egg yolk and white were highlighted, the yolk containing more SeCys and the white more SeMet. An insight into selenium bioaccessibility in eggs was obtained by digestion with simulated gastric and gastrointestinal juices and size-exclusion HPLC-ICP MS. SN - 1618-2650 UR - https://www.unboundmedicine.com/medline/citation/20229009/Determination_of_selenomethionine_selenocysteine_and_inorganic_selenium_in_eggs_by_HPLC_inductively_coupled_plasma_mass_spectrometry_ DB - PRIME DP - Unbound Medicine ER -