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Correlation of Histoplasma capsulatum polysaccharide antigen with the severity of infection in murine histoplasmosis.
J Clin Lab Anal. 1991; 5(2):121-6.JC

Abstract

We sought to determine if Histoplasma capsulatum polysaccharide antigen (HPA) levels correlate with the extent of infection in murine of histoplasmosis. Separate groups of mice were inoculated intratracheally with varying numbers of H. capsulatum yeast cells. After 1 week, HPA levels and fungal burden (quantitative culture of lung and spleen and histopathologic stain of lung) were determined in lung and spleen, and HPA levels in serum. HPA levels, cultures and histopathological stain results of lung and spleen tissue showed a direct correlation with increasing inoculum size. HPA levels in serum also correlated with the size of inoculum. H. capsulatum antigen in lung correlated with silver stain scores of lung tissue, (R = 0.948, P less than 0.001) and with quantitative culture scores of lung, (R = 0.929, P less than 0.001). HPA levels in spleen tissue also correlated with spleen culture scores, (R = 0.724, P less than 0.001). These results indicate that determination of HPA level in serum and tissue may be a useful test in evaluating the severity of diseases as well as efficacy of antifungal therapy in histoplasmosis.

Authors+Show Affiliations

Indiana University School of Medicine, Department of Medicine, Indianapolis.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

2023057

Citation

Williams, B J., et al. "Correlation of Histoplasma Capsulatum Polysaccharide Antigen With the Severity of Infection in Murine Histoplasmosis." Journal of Clinical Laboratory Analysis, vol. 5, no. 2, 1991, pp. 121-6.
Williams BJ, Connolly-Stringfield P, Bartlett M, et al. Correlation of Histoplasma capsulatum polysaccharide antigen with the severity of infection in murine histoplasmosis. J Clin Lab Anal. 1991;5(2):121-6.
Williams, B. J., Connolly-Stringfield, P., Bartlett, M., Durkin, M., Garringer, T., Blair, R., Connolly, K., Tewari, R. P., & Wheat, L. J. (1991). Correlation of Histoplasma capsulatum polysaccharide antigen with the severity of infection in murine histoplasmosis. Journal of Clinical Laboratory Analysis, 5(2), 121-6.
Williams BJ, et al. Correlation of Histoplasma Capsulatum Polysaccharide Antigen With the Severity of Infection in Murine Histoplasmosis. J Clin Lab Anal. 1991;5(2):121-6. PubMed PMID: 2023057.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Correlation of Histoplasma capsulatum polysaccharide antigen with the severity of infection in murine histoplasmosis. AU - Williams,B J, AU - Connolly-Stringfield,P, AU - Bartlett,M, AU - Durkin,M, AU - Garringer,T, AU - Blair,R, AU - Connolly,K, AU - Tewari,R P, AU - Wheat,L J, PY - 1991/1/1/pubmed PY - 1991/1/1/medline PY - 1991/1/1/entrez SP - 121 EP - 6 JF - Journal of clinical laboratory analysis JO - J Clin Lab Anal VL - 5 IS - 2 N2 - We sought to determine if Histoplasma capsulatum polysaccharide antigen (HPA) levels correlate with the extent of infection in murine of histoplasmosis. Separate groups of mice were inoculated intratracheally with varying numbers of H. capsulatum yeast cells. After 1 week, HPA levels and fungal burden (quantitative culture of lung and spleen and histopathologic stain of lung) were determined in lung and spleen, and HPA levels in serum. HPA levels, cultures and histopathological stain results of lung and spleen tissue showed a direct correlation with increasing inoculum size. HPA levels in serum also correlated with the size of inoculum. H. capsulatum antigen in lung correlated with silver stain scores of lung tissue, (R = 0.948, P less than 0.001) and with quantitative culture scores of lung, (R = 0.929, P less than 0.001). HPA levels in spleen tissue also correlated with spleen culture scores, (R = 0.724, P less than 0.001). These results indicate that determination of HPA level in serum and tissue may be a useful test in evaluating the severity of diseases as well as efficacy of antifungal therapy in histoplasmosis. SN - 0887-8013 UR - https://www.unboundmedicine.com/medline/citation/2023057/Correlation_of_Histoplasma_capsulatum_polysaccharide_antigen_with_the_severity_of_infection_in_murine_histoplasmosis_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0887-8013&date=1991&volume=5&issue=2&spage=121 DB - PRIME DP - Unbound Medicine ER -