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The multi-copper-ion oxidase CueO of Salmonella enterica serovar Typhimurium is required for systemic virulence.
Infect Immun. 2010 May; 78(5):2312-9.II

Abstract

Salmonella enterica serovar Typhimurium possesses a multi-copper-ion oxidase (multicopper oxidase), CueO (also known as CuiD), a periplasmic enzyme known to be required for resistance to copper ions. CueO from S. Typhimurium was expressed as a recombinant protein in Escherichia coli, and the purified protein exhibited a high cuprous oxidase activity. We have characterized an S. Typhimurium cueO mutant and confirmed that it is more sensitive to copper ions. Using a murine model of infection, it was observed that the cueO mutant was significantly attenuated, as indicated by reduced recovery of bacteria from liver and spleen, although there was no significant difference in recovery from Peyer's patches and mesenteric lymph nodes. However, the intracellular survival of the cueO mutant in unprimed or gamma-interferon-primed murine macrophages was not statistically different from that of wild-type Salmonella, suggesting that additional host factors are involved in clearance of the cueO mutant. Unlike a cueO mutant from E. coli, the S. Typhimurium cueO mutant did not show greater sensitivity to hydrogen peroxide and its sensitivity to copper ions was not affected by siderophores. Similarly, the S. Typhimurium cueO mutant was not rescued from copper ion toxicity by addition of the branched-chain amino acids and leucine.

Authors+Show Affiliations

School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane 4072, Australia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20231415

Citation

Achard, Maud E S., et al. "The Multi-copper-ion Oxidase CueO of Salmonella Enterica Serovar Typhimurium Is Required for Systemic Virulence." Infection and Immunity, vol. 78, no. 5, 2010, pp. 2312-9.
Achard ME, Tree JJ, Holden JA, et al. The multi-copper-ion oxidase CueO of Salmonella enterica serovar Typhimurium is required for systemic virulence. Infect Immun. 2010;78(5):2312-9.
Achard, M. E., Tree, J. J., Holden, J. A., Simpfendorfer, K. R., Wijburg, O. L., Strugnell, R. A., Schembri, M. A., Sweet, M. J., Jennings, M. P., & McEwan, A. G. (2010). The multi-copper-ion oxidase CueO of Salmonella enterica serovar Typhimurium is required for systemic virulence. Infection and Immunity, 78(5), 2312-9. https://doi.org/10.1128/IAI.01208-09
Achard ME, et al. The Multi-copper-ion Oxidase CueO of Salmonella Enterica Serovar Typhimurium Is Required for Systemic Virulence. Infect Immun. 2010;78(5):2312-9. PubMed PMID: 20231415.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The multi-copper-ion oxidase CueO of Salmonella enterica serovar Typhimurium is required for systemic virulence. AU - Achard,Maud E S, AU - Tree,Jai J, AU - Holden,James A, AU - Simpfendorfer,Kim R, AU - Wijburg,Odilia L C, AU - Strugnell,Richard A, AU - Schembri,Mark A, AU - Sweet,Matthew J, AU - Jennings,Michael P, AU - McEwan,Alastair G, Y1 - 2010/03/15/ PY - 2010/3/17/entrez PY - 2010/3/17/pubmed PY - 2010/5/4/medline SP - 2312 EP - 9 JF - Infection and immunity JO - Infect Immun VL - 78 IS - 5 N2 - Salmonella enterica serovar Typhimurium possesses a multi-copper-ion oxidase (multicopper oxidase), CueO (also known as CuiD), a periplasmic enzyme known to be required for resistance to copper ions. CueO from S. Typhimurium was expressed as a recombinant protein in Escherichia coli, and the purified protein exhibited a high cuprous oxidase activity. We have characterized an S. Typhimurium cueO mutant and confirmed that it is more sensitive to copper ions. Using a murine model of infection, it was observed that the cueO mutant was significantly attenuated, as indicated by reduced recovery of bacteria from liver and spleen, although there was no significant difference in recovery from Peyer's patches and mesenteric lymph nodes. However, the intracellular survival of the cueO mutant in unprimed or gamma-interferon-primed murine macrophages was not statistically different from that of wild-type Salmonella, suggesting that additional host factors are involved in clearance of the cueO mutant. Unlike a cueO mutant from E. coli, the S. Typhimurium cueO mutant did not show greater sensitivity to hydrogen peroxide and its sensitivity to copper ions was not affected by siderophores. Similarly, the S. Typhimurium cueO mutant was not rescued from copper ion toxicity by addition of the branched-chain amino acids and leucine. SN - 1098-5522 UR - https://www.unboundmedicine.com/medline/citation/20231415/The_multi_copper_ion_oxidase_CueO_of_Salmonella_enterica_serovar_Typhimurium_is_required_for_systemic_virulence_ L2 - http://iai.asm.org/cgi/pmidlookup?view=long&pmid=20231415 DB - PRIME DP - Unbound Medicine ER -