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Expression of osteonectin, decorin, and transforming growth factor-beta 1 genes in fibroblasts cultured from patients with systemic sclerosis and morphea.
J Rheumatol. 1991 Feb; 18(2):247-51.JR

Abstract

A characteristic feature of fibroblasts cultured from affected skin areas of patients with systemic sclerosis (SSc) and localized scleroderma (morphea) is excessive activation of collagen biosynthesis. To elucidate the nature of fibroblast activation in scleroderma we have studied the expression of 3 noncollagenous connective tissue components, osteonectin, small dermatan sulfate proteoglycan (proteoglycan II, decorin), and transforming growth factor-beta 1 (TGF-beta 1), by measuring their mRNA levels in fibroblast cultures from 6 patients with SSc and 3 with morphea. A clear correlation was observed between the increase in type I collagen and osteonectin mRNA in these cell lines. The apparent overproduction of osteonectin by scleroderma fibroblasts is in accordance with the suggested activation of osteonectin expression during tissue remodeling. The levels of decorin mRNA showed marked variation in the cell lines, but were in no correlation with collagen or osteonectin mRNA. The levels of TGF-beta 1 mRNA were found to be slightly elevated in fibroblasts grown from affected scleroderma skin. This may suggest that this potent activator of collagen production has a role during the initial activation of dermal fibroblasts both in SSc and morphea.

Authors+Show Affiliations

Department of Medical Biochemistry, University of Turku, Finland.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2023219

Citation

Vuorio, T, et al. "Expression of Osteonectin, Decorin, and Transforming Growth Factor-beta 1 Genes in Fibroblasts Cultured From Patients With Systemic Sclerosis and Morphea." The Journal of Rheumatology, vol. 18, no. 2, 1991, pp. 247-51.
Vuorio T, Kähäri VM, Black C, et al. Expression of osteonectin, decorin, and transforming growth factor-beta 1 genes in fibroblasts cultured from patients with systemic sclerosis and morphea. J Rheumatol. 1991;18(2):247-51.
Vuorio, T., Kähäri, V. M., Black, C., & Vuorio, E. (1991). Expression of osteonectin, decorin, and transforming growth factor-beta 1 genes in fibroblasts cultured from patients with systemic sclerosis and morphea. The Journal of Rheumatology, 18(2), 247-51.
Vuorio T, et al. Expression of Osteonectin, Decorin, and Transforming Growth Factor-beta 1 Genes in Fibroblasts Cultured From Patients With Systemic Sclerosis and Morphea. J Rheumatol. 1991;18(2):247-51. PubMed PMID: 2023219.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of osteonectin, decorin, and transforming growth factor-beta 1 genes in fibroblasts cultured from patients with systemic sclerosis and morphea. AU - Vuorio,T, AU - Kähäri,V M, AU - Black,C, AU - Vuorio,E, PY - 1991/2/1/pubmed PY - 1991/2/1/medline PY - 1991/2/1/entrez SP - 247 EP - 51 JF - The Journal of rheumatology JO - J Rheumatol VL - 18 IS - 2 N2 - A characteristic feature of fibroblasts cultured from affected skin areas of patients with systemic sclerosis (SSc) and localized scleroderma (morphea) is excessive activation of collagen biosynthesis. To elucidate the nature of fibroblast activation in scleroderma we have studied the expression of 3 noncollagenous connective tissue components, osteonectin, small dermatan sulfate proteoglycan (proteoglycan II, decorin), and transforming growth factor-beta 1 (TGF-beta 1), by measuring their mRNA levels in fibroblast cultures from 6 patients with SSc and 3 with morphea. A clear correlation was observed between the increase in type I collagen and osteonectin mRNA in these cell lines. The apparent overproduction of osteonectin by scleroderma fibroblasts is in accordance with the suggested activation of osteonectin expression during tissue remodeling. The levels of decorin mRNA showed marked variation in the cell lines, but were in no correlation with collagen or osteonectin mRNA. The levels of TGF-beta 1 mRNA were found to be slightly elevated in fibroblasts grown from affected scleroderma skin. This may suggest that this potent activator of collagen production has a role during the initial activation of dermal fibroblasts both in SSc and morphea. SN - 0315-162X UR - https://www.unboundmedicine.com/medline/citation/2023219/Expression_of_osteonectin_decorin_and_transforming_growth_factor_beta_1_genes_in_fibroblasts_cultured_from_patients_with_systemic_sclerosis_and_morphea_ L2 - http://www.diseaseinfosearch.org/result/2275 DB - PRIME DP - Unbound Medicine ER -