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Protective effect of mangosteen extract against beta-amyloid-induced cytotoxicity, oxidative stress and altered proteome in SK-N-SH cells.
J Proteome Res. 2010 May 07; 9(5):2076-86.JP

Abstract

Beta-amyloid (A beta) plays a key role in the pathogenesis of Alzheimer's disease (AD) by inducing neurotoxicity and cell death mainly through production of reactive oxygen species (ROS). Garcinia mangostana L. (mangosteen) has been recognized as a major source of natural antioxidants that could decrease ROS. However, its role in protection of A beta-induced cytotoxicity and apoptosis in neuronal cells remains unclear. We therefore examined such a protective effect of mangosteen extract (ME) by evaluating cell viability using MTT test, ROS level, caspase-3 activity, and cellular proteome. Treating SK-N-SH cells with 5-20 microM A beta((1-42)) for 24 h caused morphologically cytotoxic changes, decreased cell viability and increased ROS level, whereas preincubation with 50-400 microg/mL ME 30 min before the induction by A beta((1-42)) successfully prevented such cytotoxic effects in a dose-dependent manner (completely at 400 microg/mL). The A beta-induced increase in caspase-3 activity was also preventable by 400 microg/mL ME. Proteomic analysis using 2-D gel electrophoresis (n = 5 gels/group) followed by mass spectrometry revealed 63 proteins whose levels were significantly altered by A beta((1-42)) induction. Interestingly, changes in 10 proteins were successfully prevented by the ME pretreatment. In summary, we report herein the significant protective effects of ME against A beta-induced cytotoxicity, increased ROS, and increased caspase activity in SK-N-SH cells. Moreover, proteomic analysis revealed some proteins that might be responsible for these protective effects by ME. Further characterizations of these proteins may lead to identification of novel therapeutic targets for successful prevention and/or decreasing the severity of AD.

Authors+Show Affiliations

Department of Microbiology, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20232907

Citation

Moongkarndi, Primchanien, et al. "Protective Effect of Mangosteen Extract Against Beta-amyloid-induced Cytotoxicity, Oxidative Stress and Altered Proteome in SK-N-SH Cells." Journal of Proteome Research, vol. 9, no. 5, 2010, pp. 2076-86.
Moongkarndi P, Srisawat C, Saetun P, et al. Protective effect of mangosteen extract against beta-amyloid-induced cytotoxicity, oxidative stress and altered proteome in SK-N-SH cells. J Proteome Res. 2010;9(5):2076-86.
Moongkarndi, P., Srisawat, C., Saetun, P., Jantaravinid, J., Peerapittayamongkol, C., Soi-ampornkul, R., Junnu, S., Sinchaikul, S., Chen, S. T., Charoensilp, P., Thongboonkerd, V., & Neungton, N. (2010). Protective effect of mangosteen extract against beta-amyloid-induced cytotoxicity, oxidative stress and altered proteome in SK-N-SH cells. Journal of Proteome Research, 9(5), 2076-86. https://doi.org/10.1021/pr100049v
Moongkarndi P, et al. Protective Effect of Mangosteen Extract Against Beta-amyloid-induced Cytotoxicity, Oxidative Stress and Altered Proteome in SK-N-SH Cells. J Proteome Res. 2010 May 7;9(5):2076-86. PubMed PMID: 20232907.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effect of mangosteen extract against beta-amyloid-induced cytotoxicity, oxidative stress and altered proteome in SK-N-SH cells. AU - Moongkarndi,Primchanien, AU - Srisawat,Chatchawan, AU - Saetun,Putita, AU - Jantaravinid,Jiraporn, AU - Peerapittayamongkol,Chayanon, AU - Soi-ampornkul,Rungtip, AU - Junnu,Sarawut, AU - Sinchaikul,Supachok, AU - Chen,Shui-Tein, AU - Charoensilp,Patcharakajee, AU - Thongboonkerd,Visith, AU - Neungton,Neelobol, PY - 2010/3/18/entrez PY - 2010/3/18/pubmed PY - 2010/8/13/medline SP - 2076 EP - 86 JF - Journal of proteome research JO - J Proteome Res VL - 9 IS - 5 N2 - Beta-amyloid (A beta) plays a key role in the pathogenesis of Alzheimer's disease (AD) by inducing neurotoxicity and cell death mainly through production of reactive oxygen species (ROS). Garcinia mangostana L. (mangosteen) has been recognized as a major source of natural antioxidants that could decrease ROS. However, its role in protection of A beta-induced cytotoxicity and apoptosis in neuronal cells remains unclear. We therefore examined such a protective effect of mangosteen extract (ME) by evaluating cell viability using MTT test, ROS level, caspase-3 activity, and cellular proteome. Treating SK-N-SH cells with 5-20 microM A beta((1-42)) for 24 h caused morphologically cytotoxic changes, decreased cell viability and increased ROS level, whereas preincubation with 50-400 microg/mL ME 30 min before the induction by A beta((1-42)) successfully prevented such cytotoxic effects in a dose-dependent manner (completely at 400 microg/mL). The A beta-induced increase in caspase-3 activity was also preventable by 400 microg/mL ME. Proteomic analysis using 2-D gel electrophoresis (n = 5 gels/group) followed by mass spectrometry revealed 63 proteins whose levels were significantly altered by A beta((1-42)) induction. Interestingly, changes in 10 proteins were successfully prevented by the ME pretreatment. In summary, we report herein the significant protective effects of ME against A beta-induced cytotoxicity, increased ROS, and increased caspase activity in SK-N-SH cells. Moreover, proteomic analysis revealed some proteins that might be responsible for these protective effects by ME. Further characterizations of these proteins may lead to identification of novel therapeutic targets for successful prevention and/or decreasing the severity of AD. SN - 1535-3907 UR - https://www.unboundmedicine.com/medline/citation/20232907/Protective_effect_of_mangosteen_extract_against_beta_amyloid_induced_cytotoxicity_oxidative_stress_and_altered_proteome_in_SK_N_SH_cells_ L2 - https://doi.org/10.1021/pr100049v DB - PRIME DP - Unbound Medicine ER -