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Molecular cloning and expression of three polygalacturonase cDNAs from the tarnished plant bug, Lygus lineolaris.
J Insect Sci. 2008; 8:1-14.JI

Abstract

Three unique cDNAs encoding putative polygalacturonase enzymes were isolated from the tarnished plant bug, Lygus lineolaris (Palisot de Beauvois) (Hemiptera: Miridae). The three nucleotide sequences were dissimilar to one another, but the deduced amino acid sequences were similar to each other and to other polygalacturonases from insects, fungi, plants, and bacteria. Four conserved segments characteristic of polygalacturonases were present, but with some notable semiconservative substitutions. Two of four expected disulfide bridge-forming cysteine pairs were present. All three inferred protein translations included predicted signal sequences of 17 to 20 amino acids. Amplification of genomic DNA identified an intron in one of the genes, Llpg1, in the 5' untranslated region. Semiquantitative RT-PCR revealed expression in all stages of the insect except the eggs. Expression in adults, male and female, was highly variable, indicating a family of highly inducible and diverse enzymes adapted to the generalist polyphagous nature of this important pest.

Authors+Show Affiliations

Biological Control of Pests Research Unit, USDA-ARS, Stoneville, MS 38776, USA. Meg.Allen@ars.usda.govNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20233096

Citation

Allen, Margaret L., and Jeffrey A. Mertens. "Molecular Cloning and Expression of Three Polygalacturonase cDNAs From the Tarnished Plant Bug, Lygus Lineolaris." Journal of Insect Science (Online), vol. 8, 2008, pp. 1-14.
Allen ML, Mertens JA. Molecular cloning and expression of three polygalacturonase cDNAs from the tarnished plant bug, Lygus lineolaris. J Insect Sci. 2008;8:1-14.
Allen, M. L., & Mertens, J. A. (2008). Molecular cloning and expression of three polygalacturonase cDNAs from the tarnished plant bug, Lygus lineolaris. Journal of Insect Science (Online), 8, 1-14. https://doi.org/10.1673/031.008.2701
Allen ML, Mertens JA. Molecular Cloning and Expression of Three Polygalacturonase cDNAs From the Tarnished Plant Bug, Lygus Lineolaris. J Insect Sci. 2008;8:1-14. PubMed PMID: 20233096.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular cloning and expression of three polygalacturonase cDNAs from the tarnished plant bug, Lygus lineolaris. AU - Allen,Margaret L, AU - Mertens,Jeffrey A, PY - 2010/3/18/entrez PY - 2008/4/1/pubmed PY - 2010/5/26/medline SP - 1 EP - 14 JF - Journal of insect science (Online) JO - J Insect Sci VL - 8 N2 - Three unique cDNAs encoding putative polygalacturonase enzymes were isolated from the tarnished plant bug, Lygus lineolaris (Palisot de Beauvois) (Hemiptera: Miridae). The three nucleotide sequences were dissimilar to one another, but the deduced amino acid sequences were similar to each other and to other polygalacturonases from insects, fungi, plants, and bacteria. Four conserved segments characteristic of polygalacturonases were present, but with some notable semiconservative substitutions. Two of four expected disulfide bridge-forming cysteine pairs were present. All three inferred protein translations included predicted signal sequences of 17 to 20 amino acids. Amplification of genomic DNA identified an intron in one of the genes, Llpg1, in the 5' untranslated region. Semiquantitative RT-PCR revealed expression in all stages of the insect except the eggs. Expression in adults, male and female, was highly variable, indicating a family of highly inducible and diverse enzymes adapted to the generalist polyphagous nature of this important pest. SN - 1536-2442 UR - https://www.unboundmedicine.com/medline/citation/20233096/Molecular_cloning_and_expression_of_three_polygalacturonase_cDNAs_from_the_tarnished_plant_bug_Lygus_lineolaris_ L2 - https://academic.oup.com/jinsectscience/article-lookup/doi/10.1673/031.008.2701 DB - PRIME DP - Unbound Medicine ER -