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Increased levels of N(epsilon)-(carboxymethyl)lysine in epithelial lining fluid from peripheral airways in patients with chronic obstructive pulmonary disease: a pilot study.
Clin Sci (Lond). 2010 May 06; 119(3):143-9.CS

Abstract

It has been reported that small airway inflammation is closely associated with the severity of airflow limitation in COPD (chronic obstructive pulmonary disease). We tested a new method of measurement of biochemical constituents in ELF (epithelial lining fluid) obtained separately from the central or peripheral airways using a bronchoscopic microsampling technique. The present study was designed to determine the validity of measuring CML [N(epsilon)-(carboxymethyl)lysine] levels in ELF for the assessment of small airway inflammation in COPD. Ten non-smokers, ten current smokers and 16 COPD patients were included in the present study. Concentrations of CML, 8-isoprostane and IL-8 (interleukin-8) were measured in ELF separately from the central or peripheral airways. CML levels in central airways did not differ significantly, but were markedly higher in peripheral than in central airways in the three groups. However, CML levels in peripheral airways of COPD patients were significantly higher than those in non-smokers and current smokers. In COPD patients, the CML level in peripheral airways was significantly correlated with FEV1 (forced expiratory volume in 1 s) (r=-0.82, P=0.002) and FEV1/FVC (forced vital capacity) (r=-0.57, P=0.03). Moreover, CML levels in peripheral airways were significantly correlated with levels of both 8-isoprostane (r=0.76, P=0.003) and IL-8 (r=0.67, P=0.01). In conclusion, these findings suggest that elevated levels of CML in ELF from peripheral airways were observed in COPD patients, and this parameter was correlated with the severity of airflow limitation.

Authors+Show Affiliations

Department of Respiratory Medicine, Graduate School of Medicine, Osaka City University, Asahi-machi, Abenoku Osaka, Japan. kanazawa-h@med.osaka-cu.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20236091

Citation

Kanazawa, Hiroshi, et al. "Increased Levels of N(epsilon)-(carboxymethyl)lysine in Epithelial Lining Fluid From Peripheral Airways in Patients With Chronic Obstructive Pulmonary Disease: a Pilot Study." Clinical Science (London, England : 1979), vol. 119, no. 3, 2010, pp. 143-9.
Kanazawa H, Kodama T, Asai K, et al. Increased levels of N(epsilon)-(carboxymethyl)lysine in epithelial lining fluid from peripheral airways in patients with chronic obstructive pulmonary disease: a pilot study. Clin Sci (Lond). 2010;119(3):143-9.
Kanazawa, H., Kodama, T., Asai, K., Matsumura, S., & Hirata, K. (2010). Increased levels of N(epsilon)-(carboxymethyl)lysine in epithelial lining fluid from peripheral airways in patients with chronic obstructive pulmonary disease: a pilot study. Clinical Science (London, England : 1979), 119(3), 143-9. https://doi.org/10.1042/CS20100096
Kanazawa H, et al. Increased Levels of N(epsilon)-(carboxymethyl)lysine in Epithelial Lining Fluid From Peripheral Airways in Patients With Chronic Obstructive Pulmonary Disease: a Pilot Study. Clin Sci (Lond). 2010 May 6;119(3):143-9. PubMed PMID: 20236091.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Increased levels of N(epsilon)-(carboxymethyl)lysine in epithelial lining fluid from peripheral airways in patients with chronic obstructive pulmonary disease: a pilot study. AU - Kanazawa,Hiroshi, AU - Kodama,Toyoki, AU - Asai,Kazuhisa, AU - Matsumura,Saeko, AU - Hirata,Kazuto, Y1 - 2010/05/06/ PY - 2010/3/19/entrez PY - 2010/3/20/pubmed PY - 2010/9/2/medline SP - 143 EP - 9 JF - Clinical science (London, England : 1979) JO - Clin Sci (Lond) VL - 119 IS - 3 N2 - It has been reported that small airway inflammation is closely associated with the severity of airflow limitation in COPD (chronic obstructive pulmonary disease). We tested a new method of measurement of biochemical constituents in ELF (epithelial lining fluid) obtained separately from the central or peripheral airways using a bronchoscopic microsampling technique. The present study was designed to determine the validity of measuring CML [N(epsilon)-(carboxymethyl)lysine] levels in ELF for the assessment of small airway inflammation in COPD. Ten non-smokers, ten current smokers and 16 COPD patients were included in the present study. Concentrations of CML, 8-isoprostane and IL-8 (interleukin-8) were measured in ELF separately from the central or peripheral airways. CML levels in central airways did not differ significantly, but were markedly higher in peripheral than in central airways in the three groups. However, CML levels in peripheral airways of COPD patients were significantly higher than those in non-smokers and current smokers. In COPD patients, the CML level in peripheral airways was significantly correlated with FEV1 (forced expiratory volume in 1 s) (r=-0.82, P=0.002) and FEV1/FVC (forced vital capacity) (r=-0.57, P=0.03). Moreover, CML levels in peripheral airways were significantly correlated with levels of both 8-isoprostane (r=0.76, P=0.003) and IL-8 (r=0.67, P=0.01). In conclusion, these findings suggest that elevated levels of CML in ELF from peripheral airways were observed in COPD patients, and this parameter was correlated with the severity of airflow limitation. SN - 1470-8736 UR - https://www.unboundmedicine.com/medline/citation/20236091/Increased_levels_of_N_epsilon___carboxymethyl_lysine_in_epithelial_lining_fluid_from_peripheral_airways_in_patients_with_chronic_obstructive_pulmonary_disease:_a_pilot_study_ L2 - https://portlandpress.com/clinsci/article-lookup/doi/10.1042/CS20100096 DB - PRIME DP - Unbound Medicine ER -