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Qualitative screening of phenolic compounds in olive leaf extracts by hyphenated liquid chromatography and preliminary evaluation of cytotoxic activity against human breast cancer cells.
Anal Bioanal Chem. 2010 May; 397(2):643-54.AB

Abstract

In this work, high-performance liquid chromatography (HPLC) coupled to electrospray time-of-flight mass spectrometry (ESI-TOF-MS) and electrospray ion trap multiple-stage tandem mass spectrometry (ESI-IT-MS(2)) has been applied to screen phenolic compounds in olive leaf extracts. The use of a small particle size C18 column (1.8 micro) provided great resolution and made separation of a lot of isomers possible. The structural characterization was based on accurate mass data obtained by ESI-TOF-MS, and the nature of fragmentation ions were further confirmed by ESI-IT-MS(2) when possible. In addition, we employed tetrazolium salt (MTT)-based assays to assess the effects of olive leaf extracts on the growth of human tumor-derived cells. Upon this approach, we achieved an accurate profile of olive leaf phenolics along with the identification of several important isomers of secoiridoids and flavonoids. This will allow a better understanding of the complete composition of olive-leaf-bioactive compounds as well as their involvement in Olea europaea L. biochemical pathways. Importantly, olive leaf extracts exhibited dose-dependent inhibitory effects on the metabolic status (cell viability) of three breast cancer models in vitro. Since the tumoricidal activity of the extracts should be mainly attributed to the identified olive leaf phenolics, these findings warrant further investigation at the structure-function molecular level to definitely establish the anticancer value of these phytochemicals.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Fu S
Institute of Chemistry and Applications of Plant Resources, School of Biological & Food Engineering, Dalian Polytechnic University, 1 Qinggong Yuan, Ganjingzi District, Dalian, 116034, China.
Arráez-Roman D
No affiliation info available
Segura-Carretero A
No affiliation info available
Menéndez JA
No affiliation info available
Menéndez-Gutiérrez MP
No affiliation info available
Micol V
No affiliation info available
Fernández-Gutiérrez A
No affiliation info available

MeSH

Antineoplastic Agents, PhytogenicBreast NeoplasmsCarcinomaCell SurvivalChromatography, High Pressure LiquidCinnamatesFemaleFlavonoidsHumansIridoidsOleaPhenolsPlant LeavesPyransSpectrometry, Mass, Electrospray IonizationTandem Mass Spectrometry

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20238105

Citation

Fu, Shaoping, et al. "Qualitative Screening of Phenolic Compounds in Olive Leaf Extracts By Hyphenated Liquid Chromatography and Preliminary Evaluation of Cytotoxic Activity Against Human Breast Cancer Cells." Analytical and Bioanalytical Chemistry, vol. 397, no. 2, 2010, pp. 643-54.
Fu S, Arráez-Roman D, Segura-Carretero A, et al. Qualitative screening of phenolic compounds in olive leaf extracts by hyphenated liquid chromatography and preliminary evaluation of cytotoxic activity against human breast cancer cells. Anal Bioanal Chem. 2010;397(2):643-54.
Fu, S., Arráez-Roman, D., Segura-Carretero, A., Menéndez, J. A., Menéndez-Gutiérrez, M. P., Micol, V., & Fernández-Gutiérrez, A. (2010). Qualitative screening of phenolic compounds in olive leaf extracts by hyphenated liquid chromatography and preliminary evaluation of cytotoxic activity against human breast cancer cells. Analytical and Bioanalytical Chemistry, 397(2), 643-54. https://doi.org/10.1007/s00216-010-3604-0
Fu S, et al. Qualitative Screening of Phenolic Compounds in Olive Leaf Extracts By Hyphenated Liquid Chromatography and Preliminary Evaluation of Cytotoxic Activity Against Human Breast Cancer Cells. Anal Bioanal Chem. 2010;397(2):643-54. PubMed PMID: 20238105.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Qualitative screening of phenolic compounds in olive leaf extracts by hyphenated liquid chromatography and preliminary evaluation of cytotoxic activity against human breast cancer cells. AU - Fu,Shaoping, AU - Arráez-Roman,David, AU - Segura-Carretero,Antonio, AU - Menéndez,Javier A, AU - Menéndez-Gutiérrez,María P, AU - Micol,Vicente, AU - Fernández-Gutiérrez,Alberto, Y1 - 2010/03/19/ PY - 2009/12/09/received PY - 2010/02/19/accepted PY - 2010/02/16/revised PY - 2010/3/19/entrez PY - 2010/3/20/pubmed PY - 2010/7/10/medline SP - 643 EP - 54 JF - Analytical and bioanalytical chemistry JO - Anal Bioanal Chem VL - 397 IS - 2 N2 - In this work, high-performance liquid chromatography (HPLC) coupled to electrospray time-of-flight mass spectrometry (ESI-TOF-MS) and electrospray ion trap multiple-stage tandem mass spectrometry (ESI-IT-MS(2)) has been applied to screen phenolic compounds in olive leaf extracts. The use of a small particle size C18 column (1.8 micro) provided great resolution and made separation of a lot of isomers possible. The structural characterization was based on accurate mass data obtained by ESI-TOF-MS, and the nature of fragmentation ions were further confirmed by ESI-IT-MS(2) when possible. In addition, we employed tetrazolium salt (MTT)-based assays to assess the effects of olive leaf extracts on the growth of human tumor-derived cells. Upon this approach, we achieved an accurate profile of olive leaf phenolics along with the identification of several important isomers of secoiridoids and flavonoids. This will allow a better understanding of the complete composition of olive-leaf-bioactive compounds as well as their involvement in Olea europaea L. biochemical pathways. Importantly, olive leaf extracts exhibited dose-dependent inhibitory effects on the metabolic status (cell viability) of three breast cancer models in vitro. Since the tumoricidal activity of the extracts should be mainly attributed to the identified olive leaf phenolics, these findings warrant further investigation at the structure-function molecular level to definitely establish the anticancer value of these phytochemicals. SN - 1618-2650 UR - https://www.unboundmedicine.com/medline/citation/20238105/Qualitative_screening_of_phenolic_compounds_in_olive_leaf_extracts_by_hyphenated_liquid_chromatography_and_preliminary_evaluation_of_cytotoxic_activity_against_human_breast_cancer_cells_ L2 - https://dx.doi.org/10.1007/s00216-010-3604-0 DB - PRIME DP - Unbound Medicine ER -