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Stability of gentamicin sulfate and tobramycin sulfate in extemporaneously prepared ophthalmic solutions at 8 degrees C.
Am J Hosp Pharm 1991; 48(3):507-9AJ

Abstract

The stability of gentamicin sulfate and tobramycin sulfate in fortified ophthalmic solutions stored under refrigeration was studied. Fortified gentamicin ophthalmic solution and fortified tobramycin ophthalmic solution were prepared to a final theoretical concentration of 13.6 mg/mL by using commercially available ophthalmic and injectable solutions. Volumes of each solution were packaged in plastic bottles and refrigerated at 4-8 degrees C. Samples of each solution were analyzed by fluorescence polarization immunoassay on days 0 (before refrigeration), 1, 2, 3, 4, 7, 14, 28, 63, and 91. To validate the method, identical solutions were prepared, stored under refrigeration at 4-8 degrees C, and analyzed by a stability-indicating high-performance liquid chromatographic assay on days 0 (before refrigeration), 9, 28, 56, and 91. Fluorescence polarization immunoassay showed the mean concentrations of gentamicin and tobramycin on day 91 to be 104.4% and 97.4%, respectively, of the time 0 concentrations; the difference was not significant in either case. HPLC validated these results; the mean concentration of gentamicin and tobramycin on day 91 was 103.3% and 101.2%, respectively, of the mean day 0 concentrations. Gentamicin and tobramycin in ophthalmic solutions prepared by mixing ophthalmic and injectable products and stored in plastic bottles at 4-8 degrees C were stable for three months.

Authors+Show Affiliations

School of Pharmacy, Samford University, Birmingham, AL 35229.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2028997

Citation

McBride, H A., et al. "Stability of Gentamicin Sulfate and Tobramycin Sulfate in Extemporaneously Prepared Ophthalmic Solutions at 8 Degrees C." American Journal of Hospital Pharmacy, vol. 48, no. 3, 1991, pp. 507-9.
McBride HA, Martinez DR, Trang JM, et al. Stability of gentamicin sulfate and tobramycin sulfate in extemporaneously prepared ophthalmic solutions at 8 degrees C. Am J Hosp Pharm. 1991;48(3):507-9.
McBride, H. A., Martinez, D. R., Trang, J. M., Lander, R. D., & Helms, H. A. (1991). Stability of gentamicin sulfate and tobramycin sulfate in extemporaneously prepared ophthalmic solutions at 8 degrees C. American Journal of Hospital Pharmacy, 48(3), pp. 507-9.
McBride HA, et al. Stability of Gentamicin Sulfate and Tobramycin Sulfate in Extemporaneously Prepared Ophthalmic Solutions at 8 Degrees C. Am J Hosp Pharm. 1991;48(3):507-9. PubMed PMID: 2028997.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Stability of gentamicin sulfate and tobramycin sulfate in extemporaneously prepared ophthalmic solutions at 8 degrees C. AU - McBride,H A, AU - Martinez,D R, AU - Trang,J M, AU - Lander,R D, AU - Helms,H A, PY - 1991/3/1/pubmed PY - 1991/3/1/medline PY - 1991/3/1/entrez SP - 507 EP - 9 JF - American journal of hospital pharmacy JO - Am J Hosp Pharm VL - 48 IS - 3 N2 - The stability of gentamicin sulfate and tobramycin sulfate in fortified ophthalmic solutions stored under refrigeration was studied. Fortified gentamicin ophthalmic solution and fortified tobramycin ophthalmic solution were prepared to a final theoretical concentration of 13.6 mg/mL by using commercially available ophthalmic and injectable solutions. Volumes of each solution were packaged in plastic bottles and refrigerated at 4-8 degrees C. Samples of each solution were analyzed by fluorescence polarization immunoassay on days 0 (before refrigeration), 1, 2, 3, 4, 7, 14, 28, 63, and 91. To validate the method, identical solutions were prepared, stored under refrigeration at 4-8 degrees C, and analyzed by a stability-indicating high-performance liquid chromatographic assay on days 0 (before refrigeration), 9, 28, 56, and 91. Fluorescence polarization immunoassay showed the mean concentrations of gentamicin and tobramycin on day 91 to be 104.4% and 97.4%, respectively, of the time 0 concentrations; the difference was not significant in either case. HPLC validated these results; the mean concentration of gentamicin and tobramycin on day 91 was 103.3% and 101.2%, respectively, of the mean day 0 concentrations. Gentamicin and tobramycin in ophthalmic solutions prepared by mixing ophthalmic and injectable products and stored in plastic bottles at 4-8 degrees C were stable for three months. SN - 0002-9289 UR - https://www.unboundmedicine.com/medline/citation/2028997/Stability_of_gentamicin_sulfate_and_tobramycin_sulfate_in_extemporaneously_prepared_ophthalmic_solutions_at_8_degrees_C_ DB - PRIME DP - Unbound Medicine ER -