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Synthesis and application of a T-2 toxin imprinted polymer.
J Chromatogr A. 2010 Apr 23; 1217(17):2879-86.JC

Abstract

The synthesis of a T-2 toxin imprinted polymer and its application in food analysis are reported for the first time. A molecularly imprinted polymer (MIP) for the selective recognition of T-2 toxin (T-2) was synthesized by bulk polymerization. Methacrylamide and ethyleneglycol dimethacrylate were applied as functional monomer and cross-linker, respectively. Molecularly imprinted solid-phase extraction (MISPE) procedures were optimized for further application in the analysis of T-2. Scatchard plot analysis revealed that two classes of imprinted binding sites were formed in the imprinted polymer. The dissociation constant (KD) of the higher affinity binding sites was 7.0 micromol/l, while the KD of the lower affinity binding sites was 54.7 micromol/l. The performance of the MIP throughout the clean-up of spiked maize, barley and oat sample extracts was compared with the results obtained when using non-imprinted polymer, OASIS HLB and immunoaffinity columns (IAC). Depending on the food matrix and the spiked concentration, recoveries after MISPE and non-imprinted solid-phase extraction varied respectively from 60% to 73% and from 21% to 57%. Recoveries obtained after clean-up using OASIS HLB and IAC were in the range of 74-104% and 60-85%, respectively. Although highest recoveries were obtained with OASIS HLB sorbents, the designed MIP and the IAC were superior regarding selectivity, cross-reactivity, matrix effect, limits of detection (LOD) and limits of quantification (LOQ). Depending on the matrix, LOD after MISPE ranged from 0.4 microg/kg to 0.6 microg/kg and LOQ from 1.4 microg/kg to 1.9 microg/kg. LOD and LOQ after OASIS HLB clean-up varied from 0.9 microg/kg to 3.5 microg/kg and from 3.1 microg/kg to 11.7 microg/kg, respectively. The LOD and LOQ values obtained with IAC were in the range of 0.3-2.3 microg/kg and 1.0-7.7 microg/kg, respectively. Analysis of 39 naturally contaminated samples (maize, barley and oat) by liquid chromatography tandem mass spectrometry revealed that the MIP could be an excellent alternative for clean-up of contaminated food samples.

Authors+Show Affiliations

Ghent University, Faculty of Pharmaceutical Sciences, Laboratory of Food Analysis, Harelbekestraat 72, 9000 Ghent, Belgium. david.desmet@ugent.beNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20299027

Citation

De Smet, David, et al. "Synthesis and Application of a T-2 Toxin Imprinted Polymer." Journal of Chromatography. A, vol. 1217, no. 17, 2010, pp. 2879-86.
De Smet D, Monbaliu S, Dubruel P, et al. Synthesis and application of a T-2 toxin imprinted polymer. J Chromatogr A. 2010;1217(17):2879-86.
De Smet, D., Monbaliu, S., Dubruel, P., Van Peteghem, C., Schacht, E., & De Saeger, S. (2010). Synthesis and application of a T-2 toxin imprinted polymer. Journal of Chromatography. A, 1217(17), 2879-86. https://doi.org/10.1016/j.chroma.2010.02.068
De Smet D, et al. Synthesis and Application of a T-2 Toxin Imprinted Polymer. J Chromatogr A. 2010 Apr 23;1217(17):2879-86. PubMed PMID: 20299027.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Synthesis and application of a T-2 toxin imprinted polymer. AU - De Smet,David, AU - Monbaliu,Sofie, AU - Dubruel,Peter, AU - Van Peteghem,Carlos, AU - Schacht,Etienne, AU - De Saeger,Sarah, Y1 - 2010/03/03/ PY - 2009/12/21/received PY - 2010/02/19/revised PY - 2010/02/24/accepted PY - 2010/3/20/entrez PY - 2010/3/20/pubmed PY - 2010/6/23/medline SP - 2879 EP - 86 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1217 IS - 17 N2 - The synthesis of a T-2 toxin imprinted polymer and its application in food analysis are reported for the first time. A molecularly imprinted polymer (MIP) for the selective recognition of T-2 toxin (T-2) was synthesized by bulk polymerization. Methacrylamide and ethyleneglycol dimethacrylate were applied as functional monomer and cross-linker, respectively. Molecularly imprinted solid-phase extraction (MISPE) procedures were optimized for further application in the analysis of T-2. Scatchard plot analysis revealed that two classes of imprinted binding sites were formed in the imprinted polymer. The dissociation constant (KD) of the higher affinity binding sites was 7.0 micromol/l, while the KD of the lower affinity binding sites was 54.7 micromol/l. The performance of the MIP throughout the clean-up of spiked maize, barley and oat sample extracts was compared with the results obtained when using non-imprinted polymer, OASIS HLB and immunoaffinity columns (IAC). Depending on the food matrix and the spiked concentration, recoveries after MISPE and non-imprinted solid-phase extraction varied respectively from 60% to 73% and from 21% to 57%. Recoveries obtained after clean-up using OASIS HLB and IAC were in the range of 74-104% and 60-85%, respectively. Although highest recoveries were obtained with OASIS HLB sorbents, the designed MIP and the IAC were superior regarding selectivity, cross-reactivity, matrix effect, limits of detection (LOD) and limits of quantification (LOQ). Depending on the matrix, LOD after MISPE ranged from 0.4 microg/kg to 0.6 microg/kg and LOQ from 1.4 microg/kg to 1.9 microg/kg. LOD and LOQ after OASIS HLB clean-up varied from 0.9 microg/kg to 3.5 microg/kg and from 3.1 microg/kg to 11.7 microg/kg, respectively. The LOD and LOQ values obtained with IAC were in the range of 0.3-2.3 microg/kg and 1.0-7.7 microg/kg, respectively. Analysis of 39 naturally contaminated samples (maize, barley and oat) by liquid chromatography tandem mass spectrometry revealed that the MIP could be an excellent alternative for clean-up of contaminated food samples. SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/20299027/Synthesis_and_application_of_a_T_2_toxin_imprinted_polymer_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(10)00304-3 DB - PRIME DP - Unbound Medicine ER -