Tags

Type your tag names separated by a space and hit enter

[Detection of circulating antigen of Angiostrongylus cantonensis by 12D5 and 21B7 monoclonal antibodies].
Zhonghua Liu Xing Bing Xue Za Zhi. 2010 Jan; 31(1):79-82.ZL

Abstract

OBJECTIVE

To detect the rate of Angiostrongylus cantonensis infection and to study the effects of treatment so as to prepare monoclonal antibodies (McAbs), and gold immunochromatography assay (GICA) with 12D5 and 21B7 McAbs could be prepared in advance.

METHODS

Two McAbs (12D5 and 21B7) were applied to detect the circulating antigen (CAg) in the sera of rats infected with A. cantonensis and angiostrongyliasis patients respectively by double antibody sandwich ELISA. Either 12D5 or 21B7 McAbs was used as antibody and protein A was conjugated with colloid gold as the detection marker. A special pad for GICA was designed according to the reaction procedure, and CAg were detected by GICA in the sera of rats infected with A. cantonensis and angiostrongyliasis patients respectively.

RESULTS

12D5 McAb was identified as IgG1 and 21B7 McAb was IgM. Results from Western blotting showed that two McAbs could be used to identified 55 KD protein of adult worms of A. cantonensis. The detection rates of CAg in the sera of infected rats was 100% (48/48) and the detection rates of CAg in the sera of angiostrongyliasis patients was 100% (32/32). No cross-reaction to sera of patients with other infection of parasites, such as clonorchiasis, fasciolopsiasis, ancylostomiasis, ancylostomiasis, anisakiasis as well as schistosomiasis wee seen and normal sera did not react with 12D5 and 21B7 McAbs.

CONCLUSION

Results from sandwich ELISA and GICA with 12D5 and 21B7 McAbs showed high specificity and acting as detecting CAg of A. cantonensis in sera of infected animals and patients. We noticed that GICA with 12D5 and 21B7 was not only rapid and simple that without requirement of special instrument, but also rather sensitive and specific for the detection of current infection with A. cantonensis.

Authors+Show Affiliations

Shenzhen Centre for Disease Control and Prevention, Shenzhen 518020, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

20302705

Citation

Huang, Da-na, et al. "[Detection of Circulating Antigen of Angiostrongylus Cantonensis By 12D5 and 21B7 Monoclonal Antibodies]." Zhonghua Liu Xing Bing Xue Za Zhi = Zhonghua Liuxingbingxue Zazhi, vol. 31, no. 1, 2010, pp. 79-82.
Huang DN, Chen MX, Geng YJ, et al. [Detection of circulating antigen of Angiostrongylus cantonensis by 12D5 and 21B7 monoclonal antibodies]. Zhonghua Liu Xing Bing Xue Za Zhi. 2010;31(1):79-82.
Huang, D. N., Chen, M. X., Geng, Y. J., Li, X. H., Gao, S. T., & Zhang, R. L. (2010). [Detection of circulating antigen of Angiostrongylus cantonensis by 12D5 and 21B7 monoclonal antibodies]. Zhonghua Liu Xing Bing Xue Za Zhi = Zhonghua Liuxingbingxue Zazhi, 31(1), 79-82.
Huang DN, et al. [Detection of Circulating Antigen of Angiostrongylus Cantonensis By 12D5 and 21B7 Monoclonal Antibodies]. Zhonghua Liu Xing Bing Xue Za Zhi. 2010;31(1):79-82. PubMed PMID: 20302705.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Detection of circulating antigen of Angiostrongylus cantonensis by 12D5 and 21B7 monoclonal antibodies]. AU - Huang,Da-na, AU - Chen,Mu-xin, AU - Geng,Yi-jie, AU - Li,Xiao-heng, AU - Gao,Shi-tong, AU - Zhang,Ren-li, PY - 2010/3/23/entrez PY - 2010/3/23/pubmed PY - 2013/3/21/medline SP - 79 EP - 82 JF - Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi JO - Zhonghua Liu Xing Bing Xue Za Zhi VL - 31 IS - 1 N2 - OBJECTIVE: To detect the rate of Angiostrongylus cantonensis infection and to study the effects of treatment so as to prepare monoclonal antibodies (McAbs), and gold immunochromatography assay (GICA) with 12D5 and 21B7 McAbs could be prepared in advance. METHODS: Two McAbs (12D5 and 21B7) were applied to detect the circulating antigen (CAg) in the sera of rats infected with A. cantonensis and angiostrongyliasis patients respectively by double antibody sandwich ELISA. Either 12D5 or 21B7 McAbs was used as antibody and protein A was conjugated with colloid gold as the detection marker. A special pad for GICA was designed according to the reaction procedure, and CAg were detected by GICA in the sera of rats infected with A. cantonensis and angiostrongyliasis patients respectively. RESULTS: 12D5 McAb was identified as IgG1 and 21B7 McAb was IgM. Results from Western blotting showed that two McAbs could be used to identified 55 KD protein of adult worms of A. cantonensis. The detection rates of CAg in the sera of infected rats was 100% (48/48) and the detection rates of CAg in the sera of angiostrongyliasis patients was 100% (32/32). No cross-reaction to sera of patients with other infection of parasites, such as clonorchiasis, fasciolopsiasis, ancylostomiasis, ancylostomiasis, anisakiasis as well as schistosomiasis wee seen and normal sera did not react with 12D5 and 21B7 McAbs. CONCLUSION: Results from sandwich ELISA and GICA with 12D5 and 21B7 McAbs showed high specificity and acting as detecting CAg of A. cantonensis in sera of infected animals and patients. We noticed that GICA with 12D5 and 21B7 was not only rapid and simple that without requirement of special instrument, but also rather sensitive and specific for the detection of current infection with A. cantonensis. SN - 0254-6450 UR - https://www.unboundmedicine.com/medline/citation/20302705/[Detection_of_circulating_antigen_of_Angiostrongylus_cantonensis_by_12D5_and_21B7_monoclonal_antibodies]_ L2 - http://journal.yiigle.com/LinkIn.do?linkin_type=pubmed&issn=0254-6450&year=2010&vol=31&issue=1&fpage=79 DB - PRIME DP - Unbound Medicine ER -