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Detection of Mycobacterium leprae DNA in nine-banded armadillos (Dasypus novemcinctus) from the Andean region of Colombia.
Lepr Rev. 2009 Dec; 80(4):424-31.LR

Abstract

OBJECTIVE

To use DNA detection methodologies to test for M. leprae in nine-banded armadillos inhabiting forested regions located around the cities and towns where leprosy patients have been identified.

DESIGN

Ear lobe biopsies of 22 nine-banded armadillos were studied during a 2 year period. The biopsies were processed for DNA extraction and amplification by nested polymerase chain reaction (N-PCR) of a fragment of the high copy DNA locus of M. leprae known as R-LEP.

RESULTS

Nine of the 22 (40.9%) armadillos evaluated showed positive signals for M. leprae. Sequencing confirmed that PCR products were identical to the corresponding region of M. leprae DNA.

CONCLUSIONS

In Colombia, South America, the consumption of and contact with the nine-banded armadillo (Dasypus novemcinctus) are common, ignoring the fact that this animal can host and be a possible zoonotic reservoir of Mycobacterium leprae, the causal agent of leprosy. This is the first study demonstrating that M. leprae is present in nine-banded armadillos in a region of Colombia using specific DNA detection. The possibility of leprosy transmission due to contact and consumption of armadillo meat or use of blood for therapeutic purposes should be further investigated.

Authors+Show Affiliations

Instituto Colombiano de Medicina Tropical-Universidad CES, Cra. 43a No. 52 Sur 99 Sabaneta, Antioquia, Colombia. ncardona@ces.edu.coNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20306641

Citation

Cardona-Castro, N, et al. "Detection of Mycobacterium Leprae DNA in Nine-banded Armadillos (Dasypus Novemcinctus) From the Andean Region of Colombia." Leprosy Review, vol. 80, no. 4, 2009, pp. 424-31.
Cardona-Castro N, Beltrán JC, Ortiz-Bernal A, et al. Detection of Mycobacterium leprae DNA in nine-banded armadillos (Dasypus novemcinctus) from the Andean region of Colombia. Lepr Rev. 2009;80(4):424-31.
Cardona-Castro, N., Beltrán, J. C., Ortiz-Bernal, A., & Vissa, V. (2009). Detection of Mycobacterium leprae DNA in nine-banded armadillos (Dasypus novemcinctus) from the Andean region of Colombia. Leprosy Review, 80(4), 424-31.
Cardona-Castro N, et al. Detection of Mycobacterium Leprae DNA in Nine-banded Armadillos (Dasypus Novemcinctus) From the Andean Region of Colombia. Lepr Rev. 2009;80(4):424-31. PubMed PMID: 20306641.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of Mycobacterium leprae DNA in nine-banded armadillos (Dasypus novemcinctus) from the Andean region of Colombia. AU - Cardona-Castro,N, AU - Beltrán,J C, AU - Ortiz-Bernal,A, AU - Vissa,V, PY - 2010/3/24/entrez PY - 2010/3/24/pubmed PY - 2010/4/7/medline SP - 424 EP - 31 JF - Leprosy review JO - Lepr Rev VL - 80 IS - 4 N2 - OBJECTIVE: To use DNA detection methodologies to test for M. leprae in nine-banded armadillos inhabiting forested regions located around the cities and towns where leprosy patients have been identified. DESIGN: Ear lobe biopsies of 22 nine-banded armadillos were studied during a 2 year period. The biopsies were processed for DNA extraction and amplification by nested polymerase chain reaction (N-PCR) of a fragment of the high copy DNA locus of M. leprae known as R-LEP. RESULTS: Nine of the 22 (40.9%) armadillos evaluated showed positive signals for M. leprae. Sequencing confirmed that PCR products were identical to the corresponding region of M. leprae DNA. CONCLUSIONS: In Colombia, South America, the consumption of and contact with the nine-banded armadillo (Dasypus novemcinctus) are common, ignoring the fact that this animal can host and be a possible zoonotic reservoir of Mycobacterium leprae, the causal agent of leprosy. This is the first study demonstrating that M. leprae is present in nine-banded armadillos in a region of Colombia using specific DNA detection. The possibility of leprosy transmission due to contact and consumption of armadillo meat or use of blood for therapeutic purposes should be further investigated. SN - 0305-7518 UR - https://www.unboundmedicine.com/medline/citation/20306641/Detection_of_Mycobacterium_leprae_DNA_in_nine_banded_armadillos__Dasypus_novemcinctus__from_the_Andean_region_of_Colombia_ DB - PRIME DP - Unbound Medicine ER -