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Thaumatin-like protein and baker's respiratory allergy.
Ann Allergy Asthma Immunol 2010; 104(2):139-46AA

Abstract

BACKGROUND

Baker's asthma and rhinitis are among the most common occupational diseases. Inhaled cereal flours, such as wheat, especially cause this disease.

OBJECTIVE

To identify and test in vivo clinically important wheat allergens in baker's respiratory allergy in a Finnish population.

METHODS

Potential wheat allergens were purified using chromatographic methods from salt-soluble protein extracts of wheat flour and were used in skin prick tests with serial 10-fold dilutions (0.5-0.005 mg/mL). Twenty patients with baker's rhinitis, asthma, or both participated in this study. All the patients had positive skin prick test reactions and specific IgE antibodies to wheat flour. The control group consisted of 10 healthy individuals. Molecular identities of purified wheat allergens were characterized using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and tandem mass spectrometry.

RESULTS

Allergen concentrations of 0.3-0.5 mg/mL revealed that 12 patients reacted to a-amylase inhibitor (alpha-AI), 9 to peroxidase I (PI), 9 to thaumatin-like protein (TLP), and 6 to lipid transfer protein 2G (LTP2G). Conversely, with allergen concentrations of 0.05 mg/mL, 5 patients responded to alpha-AI, 3 to PI, 4 to LTP2G, and 6 to TLP. Of these, TLP and LTP2G are now observed to be new allergens associated with baker's asthma.

CONCLUSIONS

In addition to the earlier-described alpha-AI and PI, TLP and LTP2G are important in vivo wheat allergens in baker's allergies in Finland. Further studies are needed to elucidate the role of these novel wheat allergens in respiratory disorders.

Authors+Show Affiliations

Unit of Excellence for Immunotoxicology, Finnish Institute of Occupational Health, Helsinki, Finland. maili.lehto@ttl.fiNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20306817

Citation

Lehto, Maili, et al. "Thaumatin-like Protein and Baker's Respiratory Allergy." Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology, vol. 104, no. 2, 2010, pp. 139-46.
Lehto M, Airaksinen L, Puustinen A, et al. Thaumatin-like protein and baker's respiratory allergy. Ann Allergy Asthma Immunol. 2010;104(2):139-46.
Lehto, M., Airaksinen, L., Puustinen, A., Tillander, S., Hannula, S., Nyman, T., ... Lauerma, A. (2010). Thaumatin-like protein and baker's respiratory allergy. Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology, 104(2), pp. 139-46. doi:10.1016/j.anai.2009.11.062.
Lehto M, et al. Thaumatin-like Protein and Baker's Respiratory Allergy. Ann Allergy Asthma Immunol. 2010;104(2):139-46. PubMed PMID: 20306817.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Thaumatin-like protein and baker's respiratory allergy. AU - Lehto,Maili, AU - Airaksinen,Liisa, AU - Puustinen,Anne, AU - Tillander,Sari, AU - Hannula,Sari, AU - Nyman,Tuula, AU - Toskala,Elina, AU - Alenius,Harri, AU - Lauerma,Antti, PY - 2010/3/24/entrez PY - 2010/3/24/pubmed PY - 2010/5/25/medline SP - 139 EP - 46 JF - Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology JO - Ann. Allergy Asthma Immunol. VL - 104 IS - 2 N2 - BACKGROUND: Baker's asthma and rhinitis are among the most common occupational diseases. Inhaled cereal flours, such as wheat, especially cause this disease. OBJECTIVE: To identify and test in vivo clinically important wheat allergens in baker's respiratory allergy in a Finnish population. METHODS: Potential wheat allergens were purified using chromatographic methods from salt-soluble protein extracts of wheat flour and were used in skin prick tests with serial 10-fold dilutions (0.5-0.005 mg/mL). Twenty patients with baker's rhinitis, asthma, or both participated in this study. All the patients had positive skin prick test reactions and specific IgE antibodies to wheat flour. The control group consisted of 10 healthy individuals. Molecular identities of purified wheat allergens were characterized using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and tandem mass spectrometry. RESULTS: Allergen concentrations of 0.3-0.5 mg/mL revealed that 12 patients reacted to a-amylase inhibitor (alpha-AI), 9 to peroxidase I (PI), 9 to thaumatin-like protein (TLP), and 6 to lipid transfer protein 2G (LTP2G). Conversely, with allergen concentrations of 0.05 mg/mL, 5 patients responded to alpha-AI, 3 to PI, 4 to LTP2G, and 6 to TLP. Of these, TLP and LTP2G are now observed to be new allergens associated with baker's asthma. CONCLUSIONS: In addition to the earlier-described alpha-AI and PI, TLP and LTP2G are important in vivo wheat allergens in baker's allergies in Finland. Further studies are needed to elucidate the role of these novel wheat allergens in respiratory disorders. SN - 1081-1206 UR - https://www.unboundmedicine.com/medline/citation/20306817/Thaumatin_like_protein_and_baker's_respiratory_allergy_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1081-1206(09)00065-9 DB - PRIME DP - Unbound Medicine ER -