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Resveratrol attenuates hyperglycemia-mediated oxidative stress, proinflammatory cytokines and protects hepatocytes ultrastructure in streptozotocin-nicotinamide-induced experimental diabetic rats.
Chem Biol Interact. 2010 Jul 30; 186(2):200-10.CB

Abstract

The present study was hypothesized to investigate the hepatoprotective nature of resveratrol in averting hyperglycemia-mediated oxidative stress by measuring extent of oxidant stress and levels of proinflammatory cytokines and antioxidant competence in the hepatic tissues of streptozotocin-nicotinamide-induced diabetic rats. After the experimental period of 30 days, the pathophysiological markers such as serum bilirubin and hepatic aspartate transaminase (AST), alanine transaminase (ALT) and alkaline phosphatase (ALP) were studied in addition to hepatic TNF-alpha, IL-1 beta, IL-6, NF-kappaB p65 and nitric oxide (NO) levels in control and experimental groups of rats. The levels of vitamin C, vitamin E and reduced glutathione (GSH) and activities of superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx), glutathione-S-transferase (GST) and glutathione reductase (GR) were determined in the liver tissues. Extent of oxidative stress was also assessed by hepatic lipid peroxides, hydroperoxides and protein carbonyls. A portion of liver was processed for histological and ultrastructural studies. Oral administration of resveratrol (5mg/kg b.w.) to diabetic rats showed a significant decline in hepatic proinflammatory cytokines and notable attenuation in hepatic lipid peroxides, hydroperoxides and protein carbonyls. The diminished activities of hepatic enzymic antioxidants as well as the decreased levels of hepatic non-enzymic antioxidants of diabetic rats were reverted to near normalcy by resveratrol administration. Moreover, the histological and ultrastructural observations evidenced that resveratrol effectively rescues the hepatocytes from hyperglycemia-mediated oxidative damage without affecting its cellular function and structural integrity. The findings of the present investigation demonstrated the hepatocyte protective nature of resveratrol by attenuating markers of hyperglycemia-mediated oxidative stress and antioxidant competence in hepatic tissues of diabetic rats.

Authors+Show Affiliations

Department of Biochemistry, University of Madras, Guindy Campus, Chennai 600025, Tamilnadu, India. ppalsamy@gmail.comNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20307516

Citation

Palsamy, P, et al. "Resveratrol Attenuates Hyperglycemia-mediated Oxidative Stress, Proinflammatory Cytokines and Protects Hepatocytes Ultrastructure in Streptozotocin-nicotinamide-induced Experimental Diabetic Rats." Chemico-biological Interactions, vol. 186, no. 2, 2010, pp. 200-10.
Palsamy P, Sivakumar S, Subramanian S. Resveratrol attenuates hyperglycemia-mediated oxidative stress, proinflammatory cytokines and protects hepatocytes ultrastructure in streptozotocin-nicotinamide-induced experimental diabetic rats. Chem Biol Interact. 2010;186(2):200-10.
Palsamy, P., Sivakumar, S., & Subramanian, S. (2010). Resveratrol attenuates hyperglycemia-mediated oxidative stress, proinflammatory cytokines and protects hepatocytes ultrastructure in streptozotocin-nicotinamide-induced experimental diabetic rats. Chemico-biological Interactions, 186(2), 200-10. https://doi.org/10.1016/j.cbi.2010.03.028
Palsamy P, Sivakumar S, Subramanian S. Resveratrol Attenuates Hyperglycemia-mediated Oxidative Stress, Proinflammatory Cytokines and Protects Hepatocytes Ultrastructure in Streptozotocin-nicotinamide-induced Experimental Diabetic Rats. Chem Biol Interact. 2010 Jul 30;186(2):200-10. PubMed PMID: 20307516.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Resveratrol attenuates hyperglycemia-mediated oxidative stress, proinflammatory cytokines and protects hepatocytes ultrastructure in streptozotocin-nicotinamide-induced experimental diabetic rats. AU - Palsamy,P, AU - Sivakumar,S, AU - Subramanian,S, Y1 - 2010/03/20/ PY - 2010/02/17/received PY - 2010/03/09/revised PY - 2010/03/12/accepted PY - 2010/3/24/entrez PY - 2010/3/24/pubmed PY - 2010/7/2/medline SP - 200 EP - 10 JF - Chemico-biological interactions JO - Chem Biol Interact VL - 186 IS - 2 N2 - The present study was hypothesized to investigate the hepatoprotective nature of resveratrol in averting hyperglycemia-mediated oxidative stress by measuring extent of oxidant stress and levels of proinflammatory cytokines and antioxidant competence in the hepatic tissues of streptozotocin-nicotinamide-induced diabetic rats. After the experimental period of 30 days, the pathophysiological markers such as serum bilirubin and hepatic aspartate transaminase (AST), alanine transaminase (ALT) and alkaline phosphatase (ALP) were studied in addition to hepatic TNF-alpha, IL-1 beta, IL-6, NF-kappaB p65 and nitric oxide (NO) levels in control and experimental groups of rats. The levels of vitamin C, vitamin E and reduced glutathione (GSH) and activities of superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx), glutathione-S-transferase (GST) and glutathione reductase (GR) were determined in the liver tissues. Extent of oxidative stress was also assessed by hepatic lipid peroxides, hydroperoxides and protein carbonyls. A portion of liver was processed for histological and ultrastructural studies. Oral administration of resveratrol (5mg/kg b.w.) to diabetic rats showed a significant decline in hepatic proinflammatory cytokines and notable attenuation in hepatic lipid peroxides, hydroperoxides and protein carbonyls. The diminished activities of hepatic enzymic antioxidants as well as the decreased levels of hepatic non-enzymic antioxidants of diabetic rats were reverted to near normalcy by resveratrol administration. Moreover, the histological and ultrastructural observations evidenced that resveratrol effectively rescues the hepatocytes from hyperglycemia-mediated oxidative damage without affecting its cellular function and structural integrity. The findings of the present investigation demonstrated the hepatocyte protective nature of resveratrol by attenuating markers of hyperglycemia-mediated oxidative stress and antioxidant competence in hepatic tissues of diabetic rats. SN - 1872-7786 UR - https://www.unboundmedicine.com/medline/citation/20307516/Resveratrol_attenuates_hyperglycemia_mediated_oxidative_stress_proinflammatory_cytokines_and_protects_hepatocytes_ultrastructure_in_streptozotocin_nicotinamide_induced_experimental_diabetic_rats_ DB - PRIME DP - Unbound Medicine ER -